This study aimed to produce and determine physical and mechanical properties of three layers particleboards produced with Eucalyptus sp. particles, in outer layers, and Eucalyptus urograndis e Eucalyptus saligna bark, in internal layer. Recommendations of the Brazilian Code ABNT NBR 14810-3:2006 were adopted to fundament physical and mechanical tests. Properties considered were modulus of rupture (MOR) and modulus of elasticity (MOE) in static bending; internal adhesion; thickness swelling; water absorption; moisture content and density of the panels. Four different panel compositions were studied, with 5, 10, 15 and 20% of bark (related to particle mass) the internal layer. Results were compared with the requirements of: Brazilian Code ABNT NBR 14810-2; European Code EN 312 and related literature. The produced panels were adequate to all the values stipulated the cited codes and also superior to similar studies found in the literature, for all compositions of bark in internal layer, except for the moisture content test, in which only the composition of 5 % hull met the normative specifications.