Environmental survival mechanisms of the foodborne pathogen Campylobacter jejuni

Murphy, Caroline; Carroll, C.; Jordan, Kieran

doi:10.1111/j.1365-2672.2006.02903.x

Cited by 271 publications

(202 citation statements)

References 98 publications

(195 reference statements)

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“…Thus, in developing countries, the routes of transmission are complex and multifactorial and campylobacteriosis in developed countries is considered mainly a food infection whose main source is the chicken meat (EFSA, 2005). Murphy et al (2006) states that the rate of campylobacteriosis incidence in the United States in 2004 was 12.9 cases per 100,000 confirmed cases of food-borne bacterial diseases in humans, whereas in the UK the rate was 73/100.000 in 2003 and in the countries of northern Europe was 60 to 90/100.000.…”

Section: Development Epidemiologymentioning

confidence: 99%

Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

et al. 2015

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ABSTRACT:The infection by Campylobacter spp. constitutes a zoonosis of worldwide distribution, with serious repercussions for public health and a significant socio-economic impact. The Campylobacter species that most often are implicated in outbreaks of gastrointestinal disease are C. jejuni and C. coli. Both are recognized as the most important and with greater pathogenic potential from the viewpoint of food safety. This review aims to clarify and discuss, based on literature, the epidemiology of Campylobacter spp. and the main challenges in their control, to assist in better understanding of the risk posed to human health. Due to the difficulty of isolating and cultivating of Campylobacter spp., the high cost and not mandatory, laboratory diagnosis by traditional cultivation techniques is not widely used in Brazil, resulting in lack of accurate data on the occurrence of the disease in the country. This factor has been identified as responsible for underdiagnosing and underreporting of the disease, which means that data on contamination of animal products or outbreaks are not statements of actual national situation. The epidemiology of Campylobacter spp. is considered complex presenting multiple determinants in the spread of this microorganism. Chickens are important reservoirs of Campylobacter sp. and the main target of control measures, however, other vehicles of transmission, such as raw cow milk, drinking water and other foods of animal origin must also be considered in the set of measures to be taken to control disease. The infection by Campylobacter spp. cause gastroenteritis in humans and occurred by oral contact with infected animals. In fact, are pets or production and food consumption, the most important pathways. The clinical manifestations of the disease can be from mild to severe symptoms, including watery diarrhea, sometimes with blood, abdominal pain, fever, headache and nausea. Sometimes the infection can get complications after the diarrhea stops and starts a progressive paralysis that can result in respiratory failure and severe neurological dysfunction and may even lead to death. The occurrence of campylobacteriosi's outbreaks should be mandatory to the authorities of surveillance, to provide an investigation of common sources and transmission control through preventive measures , thereby minimizing the potential risks to human health.

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Section: Development Epidemiologymentioning

confidence: 99%

Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

et al. 2015

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“…In this study, we examined the expression of all 20 drug efflux genes in E. coli under the physiological condition of anaerobiosis coupled with amino acid limitation, a typical environmental perturbation encountered by enteric bacteria in human hosts (13)(14)(15)(16). We found that among the 20 efflux genes, the Cu-sensing CusCFBA efflux system (17,18) was significantly upregulated under this physiological condition, even in the absence of copper supplement.…”

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confidence: 99%

Copper Efflux Is Induced during Anaerobic Amino Acid Limitation in Escherichia coli To Protect Iron-Sulfur Cluster Enzymes and Biogenesis

et al. 2013

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bAdaptation to changing environments is essential to bacterial physiology. Here we report a unique role of the copper homeostasis system in adapting Escherichia coli to its host-relevant environment of anaerobiosis coupled with amino acid limitation. We found that expression of the copper/silver efflux pump CusCFBA was significantly upregulated during anaerobic amino acid limitation in E. coli without the supplement of exogenous copper. Inductively coupled plasma mass spectrometry analysis of the total intracellular copper content combined with transcriptional assay of the P cusC -lacZ reporter in the presence of specific Cu(I) chelators indicated that anaerobic amino acid limitation led to the accumulation of free Cu(I) in the periplasmic space of E. coli, resulting in Cu(I) toxicity. Cells lacking cusCFBA and another copper transporter, copA, under this condition displayed growth defects and reduced ATP production during fumarate respiration. Ectopic expression of the Fe-S cluster enzyme fumarate reductase (Frd), or supplementation with amino acids whose biosynthesis involves Fe-S cluster enzymes, rescued the poor growth of ⌬cusC cells. Yet, Cu(I) treatment did not impair the Frd activity in vitro. Further studies revealed that the alternative Fe-S cluster biogenesis system Suf was induced during the anaerobic amino acid limitation, and ⌬cusC enhanced this upregulation, indicating the impairment of the Fe-S cluster assembly machinery and the increased Fe-S cluster demands under this condition. Taken together, we conclude that the copper efflux system CusCFBA is induced during anaerobic amino acid limitation to protect Fe-S cluster enzymes and biogenesis from the endogenously originated Cu(I) toxicity, thus facilitating the physiological adaptation of E. coli.

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“…As a major food-borne pathogen, C. jejuni is well adapted in its mammalian and avian hosts, as well as in food animal production environments. So that it can survive in different conditions, C. jejuni has evolved multiple strategies for adaptation, including high rates of genetic variation (mediated by mutation and horizontal gene transfer) and differential gene expression (6,11,30). Indeed, previous analyses of the genomic sequences of C. jejuni revealed the presence of multiple genes encoding regulatory functions (9,14,32,35).…”

mentioning

confidence: 99%

CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo

Guo¹,

Wang²,

Shi³

et al. 2008

J Bacteriol

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Environmental survival mechanisms of the foodborne pathogen Campylobacter jejuni

Cited by 271 publications

References 98 publications

Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

Campylobacteriosis: an emerging zoonosis, underdiagnosed and underreported by public health agencies in Brazil

Copper Efflux Is Induced during Anaerobic Amino Acid Limitation in Escherichia coli To Protect Iron-Sulfur Cluster Enzymes and Biogenesis

CmeR Functions as a Pleiotropic Regulator and Is Required for Optimal Colonization of Campylobacter jejuni In Vivo

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