Enyzmed Pretreated Empty Palm Fruit Bunch for Biofuel Production

Amin, Nor Aishah Saidina; Ya’aini, Nazlina; Misson, Mailin; Haron, Roslindawati; Mohamed, Mona A.

doi:10.3923/jas.2010.1181.1186

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…After the saccharification of the pretreated EFB, 2.4 mg/mL and 2.9 mg/mL of total sugar were produced from EFB pretreated at RT and 35 °C, respectively. These results agreed with the findings of Amin et al (2010), where EFB was pretreated with lignin peroxidase. Their results demonstrated that the best delignification, approximately 70%, was achieved at room temperature.…”

Section: Effect Of Temperature On the Enzymatic Pretreatment Of Efbsupporting

confidence: 92%

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Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme

Ukaegbu¹,

Shah²,

Palliah³

et al. 2016

BioResources

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Laccase enzyme was used as a pretreatment agent to delignify empty fruit bunches (EFB) for sugar production. The degree of delignification of the biomass was assessed directly by the percentage of pre-pretreatment weight loss (%) after pretreatment and indirectly by the amount of total sugar produced after saccharification of the pretreated biomass with cellulase enzymes. Process parameters such as pretreatment time, temperature, enzyme concentration, substrate concentration, pH, and substrate size were studied using a one-factor-at-a-time (OFAT) analysis. The combined effect of temperature and pH on the pretreatment was studied using the face-centered central composite design (FCCCD) of response surface methodology (RSM). The optimized conditions for EFB pretreatment using laccase enzyme were achieved as follows: sample size, 2 mm; temperature, 25 °C; time, 4 h; substrate concentration, 5% (w/v); pH 5; and enzyme concentration, 20 IU/g of EFB. Although higher pretreatment was achieved with substrates of 1 mm size and at a temperature of 35 °C, these conditions were not considered energetically sustainable because of the need for energy during milling for sample size reduction and energy for temperature maintenance at 35 °C.

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Section: Effect Of Temperature On the Enzymatic Pretreatment Of Efbsupporting

confidence: 92%

“…Herpoël et al (2002) used laccase enzyme to pretreat wheat straw for 4 and 6 h and recorded 47% and 64.6% delignification, respectively. Amin et al (2010) also pretreated EFB with lignin peroxidase and manganese peroxidase and found maximum yields at 4 h and 3 h, respectively.…”

Section: Effect Of Time On the Enzymatic Pretreatment Of Efbmentioning

confidence: 99%

Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme

Ukaegbu¹,

Shah²,

Palliah³

et al. 2016

BioResources

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“…The cellulose contents (~48%) was almost double of xylan contents (~ 25%). Comparison of the compositional data for untreated EFB biomass with the literatures data (Amin et al, 2010;Sabiha-Hanim et al, 2011) shows higher values of Klason lignin but glucans and xylans " gures are slightly lower. Higher values of Klason lignin is mainly due to the higher presence of mineral and siliceous matters (Klason lignin includes ashes also).…”

Section: Resultsmentioning

confidence: 66%

Effect of Combinative Pretreatments on Cellulose-to-Glucose Conversion of Empty Palm Fruit Bunch (EFB)

Timilsena

Brosse

2014

J Food Sci Technol Nepal

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Various methods of pretreatments were investigated to their effect on cellulose to glucose conversion efficiency on enzymatic hydrolysis of a tropical agro-industrial waste residue (empty palm fruit bunch, EFB). Four different kinds of combinative pretreatments (autohydrolysis with and without naphthol, dilute acid prehydrolysis, soda prehydrolysis and enzymatic prehydrolysis) were tested for delignification during the first and the second steps of pretreatment. Each prehydrolysis step was seconded by the organosolv delignification with the same conditions of pretreatment. It was observed that all the combinative methods were far more efficient in delignification and enzymatic hydrolysis ability as compared to its one step counter parts. The combinative pretreatment method involving dilute acid prehydrolysis followed by organosolv delignification revealed the best result with respect to lignin removal and enzymatic hydrolysis. The resultant pulp contained very low Klason lignin (~5%) with high sugar conversion ratio (64% total reducing sugars). DOI: http://dx.doi.org/10.3126/jfstn.v7i0.10613 J. Food Sci. Technol. Nepal, Vol. 7 (81-85), 2012

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“…For biological pretreatment, oxidizing enzymes and white-rot fungi were used to degrade the lignin content in OPEFB. For example, enzymes such as lignin peroxidase (LiP) and manganese peroxidase (MnP) was used to pretreat OPEFB for fast pyrolysis and the bio-oil yield was improved from 20% to 30% [6]. Syafwina et al used white-rot fungi to pretreat OPEFB and the saccharification efficiency was improved by 150% compared to that of the untreated OPEFB [7].…”

Section: Pretreatmentmentioning

confidence: 99%

Conversion of Oil Palm Empty Fruit Bunch to Biofuels

Geng¹

2013

Liquid, Gaseous and Solid Biofuels - Conversion Techniques

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Enyzmed Pretreated Empty Palm Fruit Bunch for Biofuel Production

Cited by 10 publications

References 16 publications

Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme

Statistical Modeling and Optimization of Enzymatic Pretreatment of Empty Fruit Bunches with Laccase Enzyme

Effect of Combinative Pretreatments on Cellulose-to-Glucose Conversion of Empty Palm Fruit Bunch (EFB)

Conversion of Oil Palm Empty Fruit Bunch to Biofuels

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