Enzootic Mycobacterium avium infection in captive parma wallabies (Macropus parma) with unusual spinal cord manifestations

Two captive female Parma wallabies (Macropus parma) died after a history of flaccid paraplegia. On postmortem examination, granulomatous and suppurative osteomyelitis involving the left ischium and the lumbosacral region, with meningeal extension at the cauda equina, and caseonecrotic mastitis were the most significant changes. Multiple small nodules in the liver and spleen, and an enlargement of some lymph nodes with central caseous necrosis were also observed. Microscopically, a disseminated granulomatous inflammation with numerous multinucleate giant cells was seen. Numerous acid-fast bacilli were detected in macrophages, in multinucleated giant cells, and free in the central necrosis and suppurative exudate. After culture, polymerase chain reaction assays were carried out to detect the 65-kDa heat shock protein (Hsp65) and insertion sequences (IS)1245 and IS900. The causative agent was identified as Mycobacterium avium subsp. avium.

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“…20,28 In marsupials, only 2 reports have been recorded. 34,38 In both cases, no concomitant osteomyelitis was reported.…”

mentioning

confidence: 84%

Disseminated mycobacteriosis manifesting as paraplegia in two Parma wallabies (Macropus parma) naturally exposed to Mycobacterium avium

et al. 2015

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“…Espécies de cangurus e de cangurus wallaby, como Macropus giganteus, Macropus fuliginosus, Macropus eugenii, Macropus parma, Dendrolagus matschiei, Potorous tridactylus, são suscetíveis a infecções micobacterianas (GRIFFITH, 1928;MANN;BUSH, 1982;SCHOON et al, 1993;MONTALI et al, 1998;CLELAND et al, 2010). Estudos identificaram micobacteriose causada por M. avium subsp.…”

Section: Macropodídeosunclassified

“…Estudos identificaram micobacteriose causada por M. avium subsp. paratuberculosis (CLELAND et al, 2010), M. avium (MANN;BUSH, 1982;SCHOON et al, 1993;MONTALI et al, 1998), M. genavense (CLELAND et al, 2010 e M. intracellulare (PEET, DICKSON;NICKELS, 1982;MONTALI et al, 1998). animais apresentaram resultados negativos a partir de amostras de swab de abscesso dentário, pulmão, baço e abscesso pulmonar.…”

Section: Macropodídeosunclassified

<b>Estudo de micobactérias em animais silvestres mantidos em cativeiro</b>

Ikuta¹

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An improved method for the species‐specific assessment of mycobacteria in routinely formalin‐fixed and paraffin‐embedded tissues

Richter¹,

Schlüter²,

Duchrow³

et al. 1995

The Journal of Pathology

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A polymerase chain reaction (PCR) assay for the rapid and species-specific diagnosis of mycobacterial infections in paraffin-embedded clinical specimens was developed using oligonucleotide primers to amplify a fragment of the DNA coding for the ribosomal 16S RNA of mycobacteria. The oligonucleotide primers amplified DNA from all 14 species of mycobacteria tested. By means of a reamplification protocol, as few as one to two mycobacteria could be detected in the presence of human DNA. The method of DNA isolation and amplification was applied on sections of routinely formalin-fixed and paraffin-embedded tissues. PCR for the beta-actin gene served as a control for successful DNA isolation. Mycobacterial DNA could be detected in cases of mycobacterial infections. The mycobacterial species was determined by additional sequencing of the PCR fragment. This PCR method may be a powerful tool for the diagnosis of mycobacterial infections from histopathological material and for the assessment of those mycobacteria that cannot readily be cultured, such as Mycobacterium leprae.

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Enzootic Mycobacterium avium infection in captive parma wallabies (Macropus parma) with unusual spinal cord manifestations

Cited by 8 publications

References 4 publications

Disseminated mycobacteriosis manifesting as paraplegia in two Parma wallabies (Macropus parma) naturally exposed to Mycobacterium avium

Disseminated mycobacteriosis manifesting as paraplegia in two Parma wallabies (Macropus parma) naturally exposed to Mycobacterium avium

<b>Estudo de micobactérias em animais silvestres mantidos em cativeiro</b>

An improved method for the species‐specific assessment of mycobacteria in routinely formalin‐fixed and paraffin‐embedded tissues

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