Enzymatic Activity at the Surface of Biomaterials via Supramolecular Anchoring of Peptides: The Effect of Material Processing

Abstract

Supramolecular polymers allow for a modular approach to bioactive biomaterials. Here the effect of processing on the bioactivation of supramolecular biomaterials using a RNase S assay is investigated. Incorporation of S-peptides into supramolecular polymers by solvent casting shows a clear organic-solvent dependency. Although a significant release of the S-peptides is observed, RNase S activity can be measured indicating successful S-peptide surface immobilization. Additionally, the effect of electrospinning on the biomaterial's bioactivity is studied, showing that the fibrous meshes developed were bioactive. The results show the importance of solvent choice, and illustrate the potency of rendering supramolecular biomaterial films and meshes bioactive via a modular approach.

Supramolecular polycaprolactone nanocomposite based on functionalized hydroxyapatite

“…melt processing at relatively low temperatures), tunable mechanical strength, bioactivity, and tunable degradation behavior. 29,32,33…”

“…melt processing at relatively low temperatures), tunable mechanical strength, bioactivity, and tunable degradation behavior. 29,32,33 Recently, SP composites of PCL(UPy) 2 and micronized hydroxyapatite (mHAp), with UPy groups grafted onto the surface of HAp particles (mHApUPy), were prepared and studied for their mechanical and biological properties with some degree of improvement in the mechanical/biological properties. 32 However, the effect of HAp particles' surface area on the structure of PCL(UPy) 2 , and therefore, the mechanism of the interactions between PCL(UPy) 2 and HAp/HApUPy particles, has not been investigated.…”

Supramolecular Additive-Initiated Controlled Atom Transfer Radical Polymerization of Zwitterionic Polymers on Ureido-pyrimidinone-Based Biomaterial Surfaces

“…In the phase images, where brighter domains indicate a harder phase, the fibrous structure resulting from the self-assembly of the UPy moieties was clearly visible (Figures and S2). ,, This fibrous morphology and fiber diameter (Table S1) was not altered by incorporation of up to 10% UPy-BiB additive, except for only a few small bright spots, which can be either small aggregates of UPy-BiB additive or artifacts from incomplete dissolution of polymer and/or additive. The surfaces without initiator additive were exposed to the polymerization reaction mixture for 3, 6, and 24 h, after which the surfaces were thoroughly washed and dried before analysis.…”

“…Previously, these supramolecular materials were specifically functionalized with additives that contain matching supramolecular hydrogen-bonding motifs, for antifouling properties, − antimicrobial activity, additional bioactivity, ,− and postfunctionalization. , The addition of the matching hydrogen-bonding motif prevents excessive erosion and phase separation of peptide-functionalized additive from the bulk, while the activity of the peptides is retained. , …”

Silk Nanococoons: Bio‐Nanoreactors for Enzymatic Catalytic Reactions and Applications to Alcohol Intoxication

“…[ 5,6 ] Natural biomaterials are preferably adopted for the immobilization of enzymes due to their high biocompatibility and biodegradability. [ 7 ] Natural silkworm cocoons are constructed by silkworms to protect the encapsulated pupae against predator attacks and environmental threats. [ 8 ] Due to their unique hierarchical mesoscopic structures, cocoon silk materials exhibit outstanding mechanical properties, excellent biocompatibility, and controlled biodegradability.…”