Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing

Shimada, Yuji; Watanabe, Yomi; Sugihara, Akio; Tominaga, Yoshio

doi:10.1016/s1381-1177(02)00020-6

Cited by 639 publications

(397 citation statements)

References 20 publications

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“…Possible solutions could be CSTRs, PBRs, fluid beds, expanding bed, recirculation membrane reactors or once through reactors with static mixers. All have been tested in laboratory scale (Dossat et al, 1999;Hsu et al, 2004;Nie et al, 2006;Royon et al, 2007;Shaw et al, 2008;Shibasaki-Kitakawa et al, 2007;Shimada et al, 2002;Dubé et al, 2007;Darnoko and Cheryan, 2000). PBRs have been tested in the laboratory by Shaw et al (2008) for biodiesel production from soybean oil using n-hexane:tert-butanol (9:1, v/v) as cosolvent for methanol.…”

Section: Reactor Configurationsmentioning

confidence: 99%

“…Glycerol was removed in between beds. No solvent was used Shimada et al (2002) Two and three packed bed reactors in series Novozym 435, vegetable oil, methanol For two reactors in series with the addition of 1:1 molar alcohol to oil in the first and 2:1 alcohol in the second reactor, the enzymes were inactivated rapidly, while with three reactors in series with the addition of 1:1 molar alcohol to oil in each gave as high a conversion as 93% for 100 days of continuous operation Watanabe et al (2000) Assuming pores filled with oil and methanol diffusion through the oil could be limiting, modeling of h as a function of particle diameter for a solvent-free system has been done. As seen from Figure 4, the effectiveness factor decreases rapidly from 1 towards 0.66 in the range of particle diameters of existing commercial biocatalysts.…”

Section: Reactor Configurationsmentioning

confidence: 99%

See 1 more Smart Citation

A review of the current state of biodiesel production using enzymatic transesterification

Fjerbæk

Christensen

Norddahl

2009

Biotech & Bioengineering

667

459

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Enzymatic biodiesel production has been investigated intensively, but is presently employed industrially only in a 20,000 tons/year pilot plant in China (Du et al. [2008] Appl Microbiol Technol 79(3):331-337). This review presents a critical analysis of the current status of research in this area and accentuates the main obstacles to the widespread use of enzymes for commercial biodiesel transesterification. Improved results for enzymatic catalysis are seen with respect to increased yield, reaction time and stability, but the performance and price of the enzymes need further advances for them to become attractive industrially for biodiesel production. Critical aspects such as mass transfer limitations, use of solvents and water activity are discussed together with process considerations and evaluation of possible reactor configurations, if industrial production with enzymes is to be carried out. Results of published studies on the productivity of enzymes are also presented and compared to the use of chemical catalysts.

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Section: Reactor Configurationsmentioning

confidence: 99%

Section: Reactor Configurationsmentioning

confidence: 99%

A review of the current state of biodiesel production using enzymatic transesterification

Fjerbæk

Christensen

Norddahl

2009

Biotech & Bioengineering

667

459

View full text Add to dashboard Cite

show abstract

“…The main differences with respect to the model proposed by Malcata et al are: 1) the rate expressions contain the concentration of ethanol rather than the concentration of water; 2) a term for the reverse of the ethanolysis reaction is incorporated in the rate expressions. However, in the present study the reverse of the ethanolysis reaction can be neglected based on the findings that nearly 100 % of fatty acid ethyl esters can be achieved in similar systems [27,28]. Hence, the rate expression of the model is represented by: …”

Section: 51reaction Rate For the Ethanolysis Reaction: Uniresponsementioning

confidence: 99%

A kinetic study of the lipase-catalyzed ethanolysis of two short-chain triradylglycerols: Alkylglycerols vs. triacylglycerols

Vázquez

Fernández

Blanco

et al. 2010

Journal of Molecular Catalysis B: Enzymatic

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Lipase-catalyzed ethanolysis of two short-chain triradylglycerols, namely tributyrin and 2,3-dibutyroil-1-O-alkylglycerols, have been studied. Much faster rate of reaction for the ethanolysis of tributyrin than that of 2,3-dibutyroil-1-O-alkylglycerols was attained. A kinetic model for the rate of release of ethyl butyrate and for the inactivation of the lipase has been also studied. The parameter corresponding to the release of ethyl butyrate was one order of magnitude higher for ethanolysis of tributyrin than the corresponding of 2,3-dibutyroil-1-O-alkylglycerols.On the contrary, the stability of Novozym 435 during ethanolysis of 2,3-dibutyroil-1-O-alkylglycerols was higher than the corresponding of tributyrin.At the reaction conditions under study, both ethanolysis reactions take place with high selectivity and yield monoesterified alkylglycerols and sn-2 monobutyrin as the main acylglycerols in the reaction mixtures.

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“…Therefore biodiesel fuel has been studied intensively (Fukuda et al, 2001;Köse et al, 2002;Shimada et al, 1999Shimada et al, , 2002. The use of lipase as biocatalyst for biodiesel production has become of great interest due to its environmentfriendly properties.…”

Section: Introductionmentioning

confidence: 99%

Lipase-Catalyzed Biodiesel Production with Methyl Acetate as Acyl Acceptor

Huang

Yan

2008

Zeitschrift Für Naturforschung C

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Biodiesel is an alternative diesel fuel made from renewable biological resources. During the process of biodiesel production, lipase-catalyzed transesterification is a crucial step. However, current techniques using methanol as acyl acceptor have lower enzymatic activity; this limits the application of such techniques in large-scale biodiesel production. Furthermore, the lipid feedstock of currently available techniques is limited. In this paper, the technique of lipase-catalyzed transesterification of five different oils for biodiesel production with methyl acetate as acyl acceptor was investigated, and the transesterification reaction conditions were optimized. The operation stability of lipase under the obtained optimal conditions was further examined. The results showed that under optimal transesterification conditions, both plant oils and animal fats led to high yields of methyl ester: cotton-seed oil, 98%; rapeseed oil, 95%; soybean oil, 91%; tea-seed oil, 92%; and lard, 95%. Crude and refined cottonseed oil or lard made no significant difference in yields of methyl ester. No loss of enzymatic activity was detected for lipase after being repeatedly used for 40 cycles (ca. 800 h), which indicates that the operational stability of lipase was fairly good under these conditions. Our results suggest that cotton-seed oil, rape-seed oil and lard might substitute soybean oil as suitable lipid feedstock for biodiesel production. Our results also show that our technique is fit for various lipid feedstocks both from plants and animals, and presents a very promising way for the large-scale biodiesel production.

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Enzymatic alcoholysis for biodiesel fuel production and application of the reaction to oil processing

Cited by 639 publications

References 20 publications

A review of the current state of biodiesel production using enzymatic transesterification

A review of the current state of biodiesel production using enzymatic transesterification

A kinetic study of the lipase-catalyzed ethanolysis of two short-chain triradylglycerols: Alkylglycerols vs. triacylglycerols

Lipase-Catalyzed Biodiesel Production with Methyl Acetate as Acyl Acceptor

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