Enzymatic and chemical modification of zein for food application

Glušac, Jovana; Fishman, Ayelet

doi:10.1016/j.tifs.2021.04.024

Cited by 69 publications

(27 citation statements)

References 92 publications

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“…Zein is a hydrophobic macromolecular protein. Compared with other proteins, it is easier to extract and is economical [ 46 ]. In addition, it also has good biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Zein/Polyoxyethylene Core-Sheath Ultrathin Fibers and Their Antibacterial Food Packaging Applications

et al. 2022

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The purpose of this work is to develop a novel ultrathin fibrous membrane with a core-sheath structure as antibacterial food packaging film. Coaxial electrospinning was exploited to create the core-sheath structure, by which the delivery regulation of the active substance was achieved. Resveratrol (RE) and silver nanoparticles (AgNPs) were loaded into electrospun zein/polyethylene oxide ultrathin fibers to ensure a synergistic antibacterial performance. Under the assessments of a scanning electron microscope and transmission electron microscope, the ultrathin fiber was demonstrated to have a fine linear morphology, smooth surface and obvious core-sheath structure. X-ray diffraction and Fourier transform infrared analyses showed that RE and AgNPs coexisted in the ultrathin fibers and had good compatibility with the polymeric matrices. The water contact angle experiments were conducted to evaluate the hydrophilicity and hygroscopicity of the fibers. In vitro dissolution tests revealed that RE was released in a sustained manner. In the antibacterial experiments against Staphylococcus aureus and Escherichia coli, the diameters of the inhibition zone of the fiber were 8.89 ± 0.09 mm and 7.26 ± 0.10 mm, respectively. Finally, cherry tomatoes were selected as the packaging object and packed with fiber films. In a practical application, the fiber films effectively reduced the bacteria and decreased the quality loss of cherry tomatoes, thereby prolonging the fresh-keeping period of cherry tomatoes to 12 days. Following the protocols reported here, many new food packaging films can be similarly developed in the future.

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“…Zein is a hydrophobic macromolecular protein. Compared with other proteins, it is easier to extract and is economical [ 46 ]. In addition, it also has good biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Zein/Polyoxyethylene Core-Sheath Ultrathin Fibers and Their Antibacterial Food Packaging Applications

et al. 2022

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“…Crosslinking can improve the water stability of zein matrices. Noncytotoxic agents like polycarboxylic acids, e.g., citric acid and maleic acid, or free aldehyde-containing compounds, e.g., glucose, oxidized sucrose, glyoxal, etc., are recommended for crosslinking zein. But the main disadvantages of these methods are time consumption and the use of high temperature .…”

Section: Introductionmentioning

confidence: 99%

UV-Crosslinked Electrospun Zein/PEO Fibroporous Membranes for Wound Dressing

Surendranath

Resmi

Rekha

et al. 2022

ACS Appl. Bio Mater.

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Electrospun zein membranes are suitable for various biomedical applications. A UV-crosslinked electrospun membrane of a zein/PEO blend for wound healing application was explored in this work. The improvement in mechanical properties of the membrane after UV crosslinking was attributed to the change in protein conformation from an α-helix to a β-sheet. The circular dichroism (CD) spectra and FTIR spectra confirmed this conformational change. XRD analysis was shown to prove the amorphous nature of polymer blends with specific broad peaks at 2θ = 9° and 20°. The water vapor transmission rate (WVTR) of the membrane was found to be in the range of 1500–2000 g m–2 day–1, which was well suited with that of commercially available wound dressing material. Enough number of available functional groups like thiol, amino, and hydroxyl groups supplement a blood clotting index (BCI) to the matrix, causing 99% BCI within 4 min. A 91% cell viability result in the MTT assay with human dermal fibroblast cells confirmed the noncytotoxicity of the membrane. Tripeptides produced after the thermolysin-based hydrolysis of zein caused inhibition of TGF β1 expression and thus increased fibroblast and collagen production. The membrane stimulated 54% more collagen production compared to control cells at day 2 and caused 84% wound closure in human dermal fibroblast cells, which were desirable index markers of a potential wound care material.

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“…For instance, a series of active ingredient carriers have been prepared using prolamin and other polymers such as soluble soybean polysaccharide (Li et al ., 2019), chitosan (Shahbazarab et al ., 2018), gelatin (Ou et al ., 2019), etc . Zein, the prolamin from corn, is most widely used in coatings, plastics, textile fibres, adhesives, and nanoparticle carriers (Glusac & Fishman, 2021). However, research on the prolamin from distiller's grains is limited.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical properties and application in film preparation of prolamin from distiller's grains

Liang

et al. 2022

Int J of Food Sci Tech

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Prolamin of sorghum-based distiller's grains was extracted and its physicochemical properties were further determined. The prolamin was found to be rich in acidic amino acids, resulting in an isoelectric point of pH 4.0. High content of α-helix structure endowed the protein good thermostability with a denaturation of 123.47 °C. The prolamin was resistant to pepsin but susceptible to trypsin in vitro digestion. The nutritional evaluation score was low and the protein does not belong to a good source of food protein due to the low digestion and lack of essential amino acids. The prolamin exhibited low water-holding capacity, oil-holding capacity, foam properties, and emulsifying properties. Moreover, the protein possessed good film-forming properties, and the characteristics of high temperature resistance, low water-holding capacity and oil-holding capacity of prolamin can endow the protein film with excellent properties of water blocking, oil blocking, and high thermal stability.

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Enzymatic and chemical modification of zein for food application

Cited by 69 publications

References 92 publications

Electrospun Zein/Polyoxyethylene Core-Sheath Ultrathin Fibers and Their Antibacterial Food Packaging Applications

Electrospun Zein/Polyoxyethylene Core-Sheath Ultrathin Fibers and Their Antibacterial Food Packaging Applications

UV-Crosslinked Electrospun Zein/PEO Fibroporous Membranes for Wound Dressing

Physicochemical properties and application in film preparation of prolamin from distiller's grains

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