Enzymatic and hemolytic activity in different Candida species

Riceto, Érika Bezerra de Melo; Menezes, Ralciane de Paula; Penatti, Mário Paulo Amante; Pedroso, Reginaldo dos Santos

doi:10.1016/j.riam.2013.11.003

Cited by 54 publications

(38 citation statements)

References 15 publications

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“…All the isolates analyzed in this study presented a degree of hemolytic activity, and some strains had an HI close to the values obtained for the clinical isolates, corroborating the results reported elsewhere for clinical isolates of this species (Luo et al, 2001; Rossoni et al, 2013; Riceto et al, 2015). On the other hand, most of them had lower hemolysin production than the clinical isolates.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

et al. 2016

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Several studies have been developed regarding human health risks associated with the recreational use of beaches contaminated with domestic sewage. These wastes contain various micro-organisms, including Candida tropicalis. In this context, the objective of this study was to characterize C. tropicalis isolates from the sandy beach of Ponta Negra, Natal, Rio Grande do Norte, Brazil, regarding the expression of in vitro virulence factors, adaptation to osmotic stress and susceptibility to antifungal drugs. We analyzed 62 environmental isolates and observed a great variation among them for the various virulence factors evaluated. In general, environmental isolates were more adherent to human buccal epithelial cells (HBEC) than C. tropicalis ATCC13803 reference strain, and they also showed increased biofilm production. Most of the isolates presented wrinkled phenotypes on Spider medium (34 isolates, 54.8%). The majority of the isolates also showed higher proteinase production than control strains, but low phospholipase activity. In addition, 35 isolates (56.4%) had high hemolytic activity (hemolysis index > 0.55). With regard to C. tropicalis resistance to osmotic stress, 85.4% of the isolates were able to grow in a liquid medium containing 15% sodium chloride. The strains were highly resistant to the azoles tested (fluconazole, voriconazole and itraconazole). Fifteen strains were resistant to the three azoles tested (24.2%). Some strains were also resistant to amphotericin B (14 isolates; 22.6%), while all of them were susceptible for the echinocandins tested, except for a single strain of intermediate susceptibility to micafungin. Our results demonstrate that C. tropicalis isolated from the sand can fully express virulence attributes and showed a high persistence capacity on the coastal environment; in addition of showing high minimal inhibitory concentrations to several antifungal drugs used in current clinical practice, demonstrating that environmental isolates may have pathogenic potential.

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Section: Discussionsupporting

confidence: 91%

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

et al. 2016

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“…Thus, this putative virulence factor enables pathogen survival and persistence in the host (37). In the present study only five isolates were unable to produce HM, these findings are in agreement with previous publications (27,32,33), reflecting the importance of this key virulence attribute for C. glabrata. In fact, the hemolytic activity has been demonstrated as necessary for virulence in this yeast (38).…”

Section: Discussionsupporting

confidence: 94%

“…are CO and DA. In this study, these enzymes were not produced among the studied isolates, in contrast with scarce works (22,25,29) and in agreement with the findings communicated by Riceto et al (32).…”

Section: Discussionsupporting

confidence: 93%

“…In the present study, the researchers found that all strains showed proteolytic activity, with an elevated percentage of isolates with very strong activity of AP (97.2%). As depicted in Table 2 dida spp., possibly through damage to the host cell membrane by digestion of lipids, facilitating tissue invasion as well as nutrient acquisition (35) In this work, the researchers detected 84 isolates (78.5%) with PP activity at different production levels, with these observations agreeing closely with certain studies (26,29) and more distantly with some additional reports (21,31); as opposed, other authors did not find PP activity (23,24,27,28,30,32), even in collections of numerous strains of C. glabrata (33). Otherwise, as expected, a limited number of isolates (12.1%) exhibited ES activity, agreeing with some studies (20,28,30,31) and in contrast to the work of Figueiredo-Carvalho et al (33), who reported an incidence of 56%, and further suggested that the production of this enzyme may vary according to the clinical source or the geographic region, from which the strains were iso-lated.…”

Section: Discussionsupporting

confidence: 84%

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In Vitro Determination of Hydrolytic Enzymes and Echinocandin Susceptibility in Mexican Clinical Isolates of Candida glabrata Sensu Stricto

Treviño-Rangel

Espinosa-Pérez²,

Villanueva-Lozano

et al. 2019

Jundishapur J Microbiol

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Background: Candida glabrata is an opportunistic yeast that has emerged as a cause of human fungal disease, and is a complex of three closely related species. Until now, there is not enough information about its virulence attributes. Moreover, its resistance to echinocandins has been documented, which is a cause of clinical concern. Objectives: The objective of this study was to evaluate the in vitro production of aspartyl proteinase, phospholipase, esterase, hemolysin, DNase, and coagulase in a subset of 107 Mexican clinical isolates of C. glabrata sensu stricto, as well as to determinate its echinocandin susceptibility. Methods: The enzymatic determinations were carried out in plate assays using specific substrates, excepting coagulase, which was determined by the classic tube test. Antifungal susceptibility testing was determined in accordance with the CLSI broth microdilution method. Results: Aspartyl proteinase, hemolysin, and phospholipase were detected in 100%, 95%, and 79% of isolates, respectively. Blood isolates were associated with a very strong activity of aspartyl proteinase and hemolysin, and those recovered from vaginal swabs were associated with very strong production of aspartyl proteinase, phospholipase, and hemolysin. All isolates were susceptible to echinocandins, except one bloodstream isolate, which was resistant to echinocandins. Conclusions: A very strong activity of aspartyl proteinase and hemolysin was particularly associated with both, bloodstream, and vaginal swab isolates. Echinocandin resistance was rare.

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“…Furthermore, a study found higher phospholipase and proteinase activities in C. albicans isolates than in NCA species . A Brazilian study observed haemolytic activity in C. albicans , C. glabrata , C. tropicalis and C. parapsilosis . Finally, Abi‐chacra et al reported weak haemolytic activity and strong proteinase activity in C. parapsilosis isolates.…”

Section: Discussionmentioning

confidence: 96%

Prevalence, virulence factors and antifungal susceptibility ofCandidaspp. isolated from bloodstream infections in a tertiary care hospital in Brazil

Canela

Cardoso

Vitali

et al. 2017

Mycoses

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SummaryCandida spp. are responsible for 80% of all systemic fungal infections and are associated with high mortality rates. This study characterised 79 bloodstream isolates of C. albicans, C. glabrata, C. orthopsilosis, C. parapsilosis and C. tropicalis from patients in a Brazilian hospital. The susceptibility to amphotericin B, caspofungin, fluconazole and voriconazole was determined; virulence factor production was assessed based on haemolysin, phospholipase and proteinase activities, and the patients' clinical characteristics were analysed. C. albicans was the predominant species (44%), followed by C. glabrata (19%), C. tropicalis (19%), C. parapsilosis (14%) and C. orthopsilosis (4%). The candidemia incidence was 1.52 per 1000 admissions, and the crude mortality rate was 52%. One C. albicans isolate was resistant to fluconazole and voriconazole. Moreover, 20.2%, 2.5% and 3.8% of the isolates exhibited dosedependent susceptibility to fluconazole, voriconazole and caspofungin, respectively.In conclusion, although the C. glabrata incidence was higher than that usually described in Brazil, its increase was previously observed in studies conducted worldwide. Furthermore, the azole resistance of the C. albicans isolate could be due to previous exposure to these antifungals. These results highlight the importance of epidemiological studies and will facilitate an improved understanding of candidemia in the studied hospital. K E Y W O R D Santifungal susceptibility, Candida spp., candidemia, virulence factors

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Enzymatic and hemolytic activity in different Candida species

Cited by 54 publications

References 15 publications

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

Evaluation of Virulence Factors In vitro, Resistance to Osmotic Stress and Antifungal Susceptibility of Candida tropicalis Isolated from the Coastal Environment of Northeast Brazil

In Vitro Determination of Hydrolytic Enzymes and Echinocandin Susceptibility in Mexican Clinical Isolates of Candida glabrata Sensu Stricto

Prevalence, virulence factors and antifungal susceptibility ofCandidaspp. isolated from bloodstream infections in a tertiary care hospital in Brazil

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