“…Given that a similar accumulative pattern of MDA (Figure 4A) and ROS (Figure 4B) in CBLEO-treated S. aureus cells was positively associated with the degree of bacterial growth inhibition, material release, and cell membrane damage (Figures 1-3), it seems certain that CBLEO-induced oxidative stress may be one crucial bactericidal effect. Our results were consistent with the effect of the essential oils from different plants and natural compounds (such as carvacrol, citral, flavonoid, anthocyanin, dihydromyricetin, and eugenol) on S. flexneri, S. aureus, E. coli, and P. digitatum [7,8,14,16,17,19,28,55,56] and also evidenced by previous studies showing that the effects of antibacterial agents (such as fluconazole, cerulein, catechin, chitosan, miconazole, indomethacin, and hypocrellin A) could cause oxidative stress with the consequence of ROS accumulation and membranous damage destined for bacterial cell death [44,[57][58][59][60].…”