Enzymatic antioxidant response to low-temperature acclimation in the cyanobacterium Arthrospira platensis

Chien, Lee-Feng; Vonshak, Avigad

doi:10.1007/s10811-010-9607-6

Cited by 7 publications

(4 citation statements)

References 38 publications

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“…For example, homeoviscous adaptation, which involves the induction of polyunsaturated fatty acids synthesis at Low temperature (LT), plays a significant role in cold adaptation in cyanobacteria, including Spirulina [16,17]. Lee-Feng et al [18] demonstrated that enhanced antioxidant enzyme activities at LT (15°C) are an efficient regulatory strategic response to LT-induced photoinhibition. In addition, a previous study also indicated that the adaptation of Arthrospira platensis to cold stress involves a down-regulation of photosynthetic activity and increased resistance to photoinhibition [19].…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

Chang

Sun

et al. 2016

PLoS ONE

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Low temperature (LT) is one of the most important abiotic stresses that can significantly reduce crop yield. To gain insight into how Spirulina responds to LT stress, comprehensive physiological and proteomic analyses were conducted in this study. Significant decreases in growth and pigment levels as well as excessive accumulation of compatible osmolytes were observed in response to LT stress. An isobaric tag for relative and absolute quantitation (iTRAQ)-based quantitative proteomics approach was used to identify changes in protein abundance in Spirulina under LT. A total of 3,782 proteins were identified, of which 1,062 showed differential expression. Bioinformatics analysis indicated that differentially expressed proteins that were enriched in photosynthesis, carbohydrate metabolism, amino acid biosynthesis, and translation are important for the maintenance of cellular homeostasis and metabolic balance in Spirulina when subjected to LT stress. The up-regulation of proteins involved in gluconeogenesis, starch and sucrose metabolism, and amino acid biosynthesis served as coping mechanisms of Spirulina in response to LT stress. Moreover, the down-regulated expression of proteins involved in glycolysis, TCA cycle, pentose phosphate pathway, photosynthesis, and translation were associated with reduced energy consumption. The findings of the present study allow a better understanding of the response of Spirulina to LT stress and may facilitate in the elucidation of mechanisms underlying LT tolerance.

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Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

Chang

Sun

et al. 2016

PLoS ONE

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“…Increased activities of SOD, CAT, APX (ascorbate peroxidase), and GR provide protection against heat (43°C) stress in Anabaena doliolum Bharadwaja . The greater acclima-tion ability of Arthrospira platensis Kenya to low temperature (15°C) is related to the higher activity of SOD, CAT, DHAR (dehydroascorbate reductase), GR, and the induction of a higher number of SOD isoenzymes in this strain, as compared to A. platensis M2 (Chien & Vonshak 2011).…”

Section: Discussionmentioning

confidence: 82%

“…). The greater acclimation ability of Arthrospira platensis Kenya to low temperature (15°C) is related to the higher activity of SOD, CAT, DHAR (dehydroascorbate reductase), GR, and the induction of a higher number of SOD isoenzymes in this strain, as compared to A. platensis M2 (Chien & Vonshak ).…”

Section: Discussionmentioning

confidence: 84%

Growth, biochemical and enzymatic responses of thermal cyanobacterium Gloeocapsa sp. (Cyanophyceae) to temperature and irradiance

Gacheva

Gigova

Ivanova

et al. 2013

Phycological Research

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Summary The present study describes a strain of Gloeocapsa sp. designated as Gacheva 2007/R‐06/1, originally isolated from a geothermal flow located in Rupite, Bulgaria. To evaluate whether this cyanobacterium is locally adapted to hot environment or has the ability to tolerate lower temperatures, its growth, biochemical composition, enzyme isoforms and activity of the main antioxidant enzymes and proteases were characterized under various temperatures and two irradiance levels. The strain was able to grow over the whole temperature range (15–40°C) under two different photon fluence densities – 132 μmol photons m−2 s−1 (unilateral, low light, LL) and 2 × 132 μmol photons m−2 s−1 (bilateral, high light, HL). The best growth occurred at either 34°C and LL or at 36°C and HL, but significant growth inhibition was noted at 15°C and 40°C. Low temperature treatment (15°C) resulted in higher levels of total protein and an increased activity of manganese superoxide dismutase (MnSOD) and glutathione reductase, as compared to optimum growth temperatures. After simultaneous exposure to 15°C and HL, increases in lipid content and activity of iron superoxide dismutase and catalase (CAT) were also observed. Cultivation of cells at 40°C enhanced MnSOD, CAT and peroxidase activities, regardless of irradiance level. Increased total protein content and protease activity at 40°C was only associated with the HL treatment. Overall, these results indicate that Gloeocapsa sp. strain Gacheva 2007/R‐06/1 used different strategies to enable cells to efficiently acclimate and withstand adverse low or high temperatures. This strain obviously tolerates a wide range of temperatures below its natural habitat temperature, and does not seem to be locally adapted to its original thermal regime. It behaved as a thermotolerant rather than a thermophilic cyanobacterium, which suggests its wider distribution in nature.

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“…2016). The main role of antioxidants is to reduce the levels of harmful reactive oxygen species (ROS) which are produced as a result of stress and consequently prevent the damage of cellular membranes by lipid peroxidation (Chien and Vonshak 2011; Ara et al . 2013; Song et al .…”

Section: Introductionmentioning

confidence: 99%

Gene transcription and antioxidants production in Arthrospira (Spirulina) platensis grown under temperature variation

Ismaiel¹,

Piercey‐Normore

2020

J. Appl. Microbiol.

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Aim To investigate the transcription of selected antioxidants and relevant genes under varying temperature conditions, and to identify the optimum temperature for antioxidants production by Arthrospira platensis. Methods and Results The dry weight (DW), pigment production, antioxidants production and gene transcription were examined in A. platensis growing under three temperatures of 23, 30 and 37°C. The cyanobacterial DW was highest in the high temperatures (30 and 37°C), while the pigments, such as Chl a, carotenoids, C‐phycocyanin and total phycobiliprotein contents, showed their maximum value at 30°C. The total soluble protein and carbohydrate contents were highest at 30°C. Lipid peroxidation, as a marker for thermal stress, was high at 23°C, while higher temperatures remarkably reduced lipid peroxidation levels. Antioxidants activity was increased by 1·5‐fold at 30°C and temperature fluctuations induced the antioxidant enzyme activities. The transcriptional abundance of heat shock protein (HSP90), glutamate synthase (GOGAT), delta‐9 desaturase (desC), iron‐superoxide dismutase (FeSOD) and the large subunit of Rubisco (rbcL) genes was measured under the same temperatures. Conclusion The optimal temperature for growth, biochemical constituents and antioxidants of A. platensis is 30°C while some antioxidant enzyme activity increased at lower and higher temperatures. Significance and Impact of the Study The study showed the significance of temperature for growth, enzymatic and non‐enzymatic antioxidants and gene expression in A. platensis. This contributes to the knowledge of culturing A. platensis to harvest specific antioxidants or as an antioxidant‐rich food source.

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Enzymatic antioxidant response to low-temperature acclimation in the cyanobacterium Arthrospira platensis

Cited by 7 publications

References 38 publications

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

iTRAQ-Based Quantitative Proteomic Analysis of Spirulina platensis in Response to Low Temperature Stress

Growth, biochemical and enzymatic responses of thermal cyanobacterium Gloeocapsa sp. (Cyanophyceae) to temperature and irradiance

Gene transcription and antioxidants production in Arthrospira (Spirulina) platensis grown under temperature variation

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