Enzymatic characterization, molecular dynamics simulation, and application of a novel Bacillus licheniformis laccase

Li, Tao; Wang, Hongbin; Li, Jingwen; Jiang, Luying; Kang, Hongwei; Guo, Zehui; Wang, Chen; Yang, Wei; Liu, Fufeng; Lu, Fuping; Liu, Yihan

doi:10.1016/j.ijbiomac.2020.11.093

Cited by 24 publications

(14 citation statements)

References 74 publications

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“…Bacillus such as B. aryabhattai and B. altitudinis were reported for cellulolytic activity studies [ 57 , 58 ], but the bacterial laccases are relatively newer and their characterization followed to that of their counterparts in fungi. Laccase activities of B. subtilis and B. licheniformis were previously studied [ 59 , 60 ]. Selected 3 filamentous fungi that were identified belong to T. atroviride and Talaromyces funiculosus .…”

Section: Resultsmentioning

confidence: 99%

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Zhang

Shah

Mohamed

et al. 2021

BioMed Research International

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Lignocellulosic waste is the most abundant biorenewable biomass on earth, and its hydrolysis releases highly valued reducing sugars. However, the presence of lignin in the biopolymeric structure makes it highly resistant to solubilization thereby hindering the hydrolysis of cellulose and hemicellulose. Microorganisms are known for their potential complex enzymes that play a dominant role in lignocellulose conversion. Therefore, the current study was designed to isolate and screen potential microorganisms for their selective delignification ability for the pretreatment of lignocellulosic biomass. An extensive isolation and screening procedure yielded 36 desired isolates (22 bacteria, 7 basidiomycete fungi, and 7 filamentous fungi). Submerged cultivation of these desired microorganisms revealed 4 bacteria and 10 fungi with potent lignocellulolytic enzyme activities. The potent isolates were identified as Pleurotus, Trichoderma, Talaromyces, Bacillus, and Chryseobacterium spp. confirmed by morphological and molecular identification. The efficiency of these strains was determined through enzyme activities, and the degraded substrates were analyzed through scanning electron microscopy (SEM) and X-ray diffraction (XRD). Among all isolated microbes, Pleurotus spp. were found to have high laccase activity. The cellulose-decomposing and selective delignification strains were subjected to solid-state fermentation (SSF). SSF of field waste corn stalks as a single-carbon source provides Pleurotus spp. better condition for the secretion of ligninolytic enzymes. These isolated ligninolytic enzymes producing microorganisms may be used for the effective pretreatment of lignocellulosic agricultural wastes for the production of high value-added natural products by fermentation.

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Section: Resultsmentioning

confidence: 99%

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Zhang

Shah

Mohamed

et al. 2021

BioMed Research International

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“…The slight inhibition of EDTA may be due to its low accessibility to the active copper sites (essential to laccase catalysis), while the effect of SDS could be explained by the partial unfolding of compact protein structure, improving the hydrophobic interactions between the substrate and the enzyme active site (Mtibaa et al 2018 ). Compared with many bacterial laccases (Li et al 2021 ; Mehandia et al 2020 ; Safary et al 2016 ), CGLac showed better resistance to the putative inhibitors. The stability of laccases possibly relates to their native sensitivity to inhibitors.…”

Section: Discussionmentioning

confidence: 87%

“…4 a. CGLac maintained highly relative activity (> 60%) at a wide range of 30–75 °C but reduced drastically above 80 °C. The thermal inactivation links to the structural stability of the laccase because high temperatures can directly change the secondary structure of laccase and thus alter laccase stability (Li et al 2021 ). Nevertheless, CGLac showed high activity within the range from room temperature to high temperature.…”

Section: Resultsmentioning

confidence: 99%

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Green production of a yellow laccase by Coriolopsis gallica for phenolic pollutants removal

Cen

Cao

et al. 2022

AMB Expr

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As a group of green biocatalysts, fungal laccases have aroused great interest in diverse biotechnological fields. Therein, yellow laccase has advantages over blue laccase in catalytic performance, but it is not common in the reported fungal laccases. Here, we report a yellow laccase from white-rot fungus Coriolopsis gallica NCULAC F1 about its production, purification, characterization, and application. Laccase production in the co-fermentation of pomelo peel and wheat bran reached the enzyme activity by 10,690 U/L after 5 days with a 13.58-time increase. After three steps of purification, laccase increased the specific activity from 30.78 to 188.79 U/mg protein with an activity recovery of 45.64%. The purified C. gallica laccase (CGLac) showed a molecular mass of about 57 kDa. CGLac had a yellow color and no absorption peaks at 610 nm and 330 nm, suggesting that it’s a yellow laccase. CGLac exhibited stability towards temperature (40–60 °C) and neutral pH (6.0–8.0). Fe3+ and Mn2+ strongly stimulated CGLac activity by 162.56% and 226.05%, respectively. CGLac remained high activities when exposed to organic reagents and putative inhibitors. Additionally, CGLac contributed to 90.78%, 93.26%, and 99.66% removal of phenol, p-chlorophenol and bisphenol A after 120 min, respectively. In conclusion, a green efficient production strategy was introduced for fungal laccase, and the obtained CGLac presented great enzymatic properties and catalytic potential in the removal of phenolic pollutants.

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“…Next, we highlighted progress in cell-free systems and synthetic microbial consortia, which are promising for biomanufacturing 4.0 as alternative forms of chassis. Finally, the challenges in the industrial applications of chassis and the next-generation chassis were analyzed, which may also be applicable for engineering chassis of other species like B. licheniformis for biomanufacturing [16].…”

Section: Introductionmentioning

confidence: 99%

Bacillus subtilis Chassis in Biomanufacturing 4.0

Zhang

Zhu

Wang

et al. 2021

Preprint

Self Cite

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Synthetic biology, an emerging research field, can promote biomanufacturing by offering various efficient chassis. Engineering Bacillus subtilis, an important workhorse in industrial biotechnology, through synthetic biology approaches may be a disruptive innovation. Advancements in chassis engineering, a synthetic biology strategy for genome-reduced cell factories, cell-free systems, and synthetic microbial consortia would be a driving force facilitating microbial production. We discussed chassis engineering categories and applications for B. subtilis. Prospects and challenges for chassis engineering in B. subtilis were also analyzed in this review article.

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Enzymatic characterization, molecular dynamics simulation, and application of a novel Bacillus licheniformis laccase

Cited by 24 publications

References 74 publications

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Isolation and Screening of Microorganisms for the Effective Pretreatment of Lignocellulosic Agricultural Wastes

Green production of a yellow laccase by Coriolopsis gallica for phenolic pollutants removal

Bacillus subtilis Chassis in Biomanufacturing 4.0

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