Enzymatic Determination of Ammonia in Lake Water Using a Semi-Automatic Analyser

Hashimoto, Shinichi; Sun, Huayu; Otsuki, Akira; Kawakami, Shinsuke; Murata, F.; Yokomizo, M.

doi:10.1080/03067319208027396

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…Ammonium in environmental waters is generally determined by indophenol blue spectrophotometry [6]. Other methods include, flow injection analysis (FIA) [7][8][9], ion chromatography (IC) [10], enzymatic method [11], spectrophotometry [12,13], sequential injection analysis (SIA) [14,15], and gas chromatography (GC)-mass spectrometry (MS) [16]. Recently, a high-capacity cation-exchange column was developed to provide better resolution of ammonium from sodium for IC analysis of environmental waters [17].…”

Section: Introductionmentioning

confidence: 99%

Determination of ammonium in river water and sewage samples by capillary zone electrophoresis with direct UV detection

Fukushi

Ito

Kimura

et al. 2006

Journal of Chromatography A

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We developed capillary zone electrophoresis (CZE) with direct UV detection for determination of ammonium in environmental water samples. Ammonium in the samples was partly converted into ammonia in the alkaline background electrolyte (BGE) during migration and was detected by molecular absorption of ammonia at 190 nm in approximately 7 min. The limit of detection (LOD) for ammonium was 0.24 mg/l (as nitrogen) at a signal-to-noise ratio of three. The respective values of the relative standard deviation (RSD) of peak area, peak height, and migration time for ammonium were 2.1, 1.8, and 0.46%. Major alkali and alkaline earth metal ions coexisting in the samples did not interfere with ammonium determination by the proposed method. The proposed method determined ammonium in surface water and sewage samples. The results were compared to those obtained using ion chromatography (IC).

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Section: Introductionmentioning

confidence: 99%

Determination of ammonium in river water and sewage samples by capillary zone electrophoresis with direct UV detection

Fukushi

Ito

Kimura

et al. 2006

Journal of Chromatography A

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“…The determination of ammonium in the environmental samples employing enzymatic reactions has been reported previously [7,10]. The applications of diaphorase coupled with tetrazolium salts, which produce highly colored formazan dyes upon NADH oxidation, have been used as chromogenic reagents for quantification of NADH as detection signal [15].…”

Section: Optimization Of An Optical Assay For Ammonium Employing the mentioning

confidence: 99%

“…The reaction involved the use of enzyme glutamate dehydrogenase (GLDH), which requires the cofactor β-nicotinamide adenine dinucleotide (NADH) and ammonium ( 4 ) in the enzymatic conversion of 2-oxoglutarate to L-glutamate [8][9][10]. During the reaction NADH is oxidized to NAD + , thereby making possible the indirect monitoring of ammonium by measuring the consumption of NADH either amperometrically at a potential of +0.76 V or optically at a wavelength of 340 nm.…”

Section: Introductionmentioning

confidence: 99%

An Optical Based Biosensor for the Determination of Ammonium in Aqueous Environment

Azmi¹,

Abdullah²,

Ahmad³

et al. 2012

AJAC

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A simple and rapid optical biosensor for the determination of ammonium was developed by immobilization of glutamate dehydrogenase (GLDH) and diaphorase (Dph) in chitosan film coated on a glass slide employing thiazolyl blue tetrazolium bromide (MTT) as a color indicator. The developed biosensor displays a purple color formation of formazan attributed to the unreacted NADH in the reaction system in the presence of ammonium. The color intensity was found to decrease proportionally with the increase of ammonium concentrations after 10 min exposure. The linearity of the biosensor towards ammonium was in the range of 16.8 -70 µM (R 2 = 0.9955) with detection limit of 11 µM. A good agreement (R 2 = 0.9984) with indothymol method was obtained in the measurement of fish pond water samples. The effect of potential interferences such as metals ion has also been evaluated.

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Development of an amperometric assay for NH+4 based on a chemically modified screen-printed NADH sensor

Abass

Hart

Cowell

et al. 1998

Analytica Chimica Acta

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Enzymatic Determination of Ammonia in Lake Water Using a Semi-Automatic Analyser

Cited by 5 publications

References 16 publications

Determination of ammonium in river water and sewage samples by capillary zone electrophoresis with direct UV detection

Determination of ammonium in river water and sewage samples by capillary zone electrophoresis with direct UV detection

An Optical Based Biosensor for the Determination of Ammonium in Aqueous Environment

Development of an amperometric assay for NH+4 based on a chemically modified screen-printed NADH sensor

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