Enzymatic Extraction of Wheat Germ Oil

Abstract

In this study enzymatic extraction of oil from wheat germ (WG) was investigated. Four enzymes (Viscozyme L, Multifect CX 13L, Multifect CX GC and Alcalase 2.4L FG) were screened for their efficacy to release oil from WG. Alcalase 2.4L FG treatment of WG improved oil extraction yield as compared to a control (aqueous extraction without enzyme). Alcalase 2.4L FG, which resulted in significantly higher oil yield than the other three enzymes, was chosen for optimization of the enzymatic oil extraction process by using Response Surface Methodology (RSM). Three processing parameters, liquid/solid ratio, extraction time and enzyme concentration, were investigated as the independent variables. Based on the experimental results, the highest oil yield, 66.5% (w/w), was obtained under the following conditions; liquid/solid ratio 16.5, enzyme concentration 1.1% and extraction time 19.25 h. A cubic model with R 2 of 0.91 was developed to describe the enzymatic extraction process. Although the cubic model predicted WG oil extraction yields well within the processing conditions studied in this study it was not effective beyond the experimental range. Further research focusing on high liquid/solid ratio, 16-20, and extraction time in 18-24 h and 0.5-5 h ranges is necessary to improve the model developed in this study.

Techno‐economic analysis of biodiesel and ethanol co‐production from lipid‐producing sugarcane

“…Protease enzyme is added at 0.5% concentration, to break down proteins, including oleosin that surrounds the lipid bodies . Enzymes have been successfully applied to enhance aqueous extraction of lipids from different biomass materials . The extracted juice contains water, lipids, sugar, and impurities such as fiber fragments, minerals, and soil particles.…”

Evaluation of the quantity and composition of sugars and lipid in the juice and bagasse of lipid producing sugarcane

“…The lipid contents in juice were calculated by comparing the amount of lipids in the sugarcane stems and in the bagasse. The sugar and lipid extraction efficiencies from the sugarcane stems were calculated by the following equations as provided in the literature (Xie et al, 2011):…”

Enzymatic Extraction of Oil fromBalanites Aegyptiaca(Desert Date) Kernel and Comparison with Solvent Extracted Oil

“…Enzymatic treatment using optimized conditions is required to break cell wall structures and increase oil yield (Xie et al . ). Enzyme mixtures containing cellulases, hemicellulases, pectinases and proteases are quite effective in different vegetable oil extraction processes (Abdulkarim et al .…”