Enzymatic Hydrolysate of Cinnamon Waste Material as Feedstock for the Microbial Production of Carotenoids

Abstract

In the context of the global need to move towards circular economies, microbial cell factories can be employed thanks to their ability to use side-stream biomasses from the agro-industrial sector to obtain additional products. The valorization of residues allows for better and complete use of natural resources and, at the same time, for the avoidance of waste management to address our needs. In this work, we focused our attention on the microbial valorization of cinnamon waste material after polyphenol extraction (C-PEW) (Cinnamomum verum J.Presl), generally discarded without any additional processing. The sugars embedded in C-PEW were released by enzymatic hydrolysis, more compatible than acid hydrolysis with the subsequent microbial cultivation. We demonstrated that the yeast Rhodosporidium toruloides was able to grow and produce up to 2.00 (±0.23) mg/L of carotenoids in the resulting hydrolysate as a sole carbon and nitrogen source despite the presence of antimicrobial compounds typical of cinnamon. To further extend the potential of our finding, we tested other fungal cell factories for growth on the same media. Overall, these results are opening the possibility to develop separate hydrolysis and fermentation (SHF) bioprocesses based on C-PEW and microbial biotransformation to obtain high-value molecules.

Use of environmentally safe micromycetes of the genus <i>Rhodotorula</i> to obtain fodder carotene‐containing concentrate

“…Синтез в ней липидов, например, с культурой R. gracilis обеспечивал их количество 21,1 г/100 г, каротиноидов -360,4 мкг/г биомассы, полиненасыщенных жирных кислот -30,4% от общего количества [33; 34]. При трансформации отходов корицы дрожжами Rhodosporidium toruloides, остающихся после удаления полифенолов, количество каротиноидов составляло 2,00±0,23 мг/дм 3 [35]. Продуктивность ассоциации дрожжей R. rubra и Kluyveromyces lactis на ультрафильтрате молочной сыворотки составляла 24,3 г/дм 3 , каротиноидов -10,2 мг/дм 3 [36].…”

Biokatalytische Entstehung von Sesquiterpenoiden

“…Several chemical and physical factors may affect the production of sesquiterpenoids in submerged, cultured basidiomycetes; however, scarce information was found in literature (Ezediokpu et al, 2022). The use and valorization of food and agro-industrial side-streams as cultivation substrates may render fungal bioprocesses more sustainable (Bertacchi et al, 2021;Di Lena et al, 2020;Moreno-González & Ottens, 2021), as these fungi thrive well on such complex substrates. In this work, a variety of side-streams of different chemical compositions were used as supplements for the submerged cultivation of Cun.…”

An Environmentally-Friendly Approach for the Release of Essential Fatty Acids from Cereal By-products Using Cellulose-Degrading Enzymes<strong> </strong>

“…Contrary to these methods, biorefining of lignocellulosic waste material due to its simplicity in operational and process conditions has been receiving tremendous interest amongst researchers as a potential strategy suitable for the enhancement of physical and physicalchemical features as well as production of biomolecules and chemicals [21,22]. Biorefining by enzymatic hydrolysis (EH) is an environmentally-friendly alternative that could ensure sustainability and circularity through the utilization of renewable raw materials and foodgrade enzymes for obtaining high-added-value products and energy from biomass [23].…”