Enzymatic Hydrolysis and Ethanol Fermentation of By-Products from Potato Processing Plants

Abstract

By-products from potato processing plants were liquefied and partially saccharified using three commercially available enzymes, followed by ethanol fermentation by yeast and then hydrolysis by glucoamylase. From 12% (w/w) of fresh potato peel, about 20 mg/mL ethanol was formed in the supernatant via amylase, pectinase, or an enzyme complex; this yield was slightly increased with combinations of these enzymes. Supplementation of substandard mash to potato peel (1:1) as a raw material enhanced the amount of ethanol formation, increasing it to approximately 50 mg/mL by the mixture of all three enzymes. After the addition of yeast and glucoamylase to the partially saccharified material, ethanol formation proceeded gradually and slowed in 12 h with the consumption of fermentable sugars. Galacturonic acid derived from pectin was not fermented and remained in the fermented broth. In the by-products, the conversion rate of sugars to ethanol was estimated to be 42.5%.

Enhancement of ethanol production from potato-processing wastewater by engineering Escherichia coli using Vitreoscilla haemoglobin

“…For hydrolysis (method modified from Yamada et al . ), α‐amylase (EC 3·2·1·1) for liquefaction and amyloglucosidase (EC 3·2·1·3) for saccharification were used. These enzymes were obtained from Novozyme Corporation and distributed by Sigma‐Aldrich (St Louis, MO, USA).…”

“…Potato wastewater was donated by Kraft, Inc., Turkey; it was a by-product of potato chip production. For hydrolysis (method modified from Yamada et al 2009), a-amylase (EC 3Á2Á1Á1) for liquefaction and amyloglucosidase (EC 3Á2Á1Á3) for saccharification were used. These enzymes were obtained from Novozyme Corporation and distributed by Sigma-Aldrich (St Louis, MO, USA).…”

Bioethanol production from starchy part of tuberous plant (potato) using Saccharomyces cerevisiae MTCC-170

“…Therefore, the waste from potato industry can also be utilized as growth media (economical carbon source) for the fermentation processes for the production of ethanol as it has high starch content. The wastes of potato industry are currently being utilized as animal feed (Yamada et al, 2009).…”

Biochemical studies on the production of biofuel (bioethanol) from potato peels wastes by Saccharomyces cerevisiae: effects of fermentation periods and nitrogen source concentration

“…Potatoes are usually peeled during processing. [5,46,47] Consequently, there is a pungent need to find an alternative productive use of the peels. One area of possibilities is to investigate the potential of potato peels wastes (PPW) for bioethanol production.…”