Enzymatic hydrolysis of brewer’s spent grain to obtain fermentable sugars

Paz, Alicia; Outeiriño, David; Guerra, Nelson Pérez; Domínguez, José Manuel

doi:10.1016/j.biortech.2018.12.082

Cited by 59 publications

(43 citation statements)

References 36 publications

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“…This work is mainly focused on the BSG extractives valorization by solvent extraction optimization and further centrifuge ultrafiltration concentration. According to the literature, the amount of extractives from different BSG sources were found to vary between 10.7 and 14.4 g/100 g in a dry basis [4,5]. Valorization of phenolic compounds found in the extractive fraction, offers a great potential due to their antioxidant properties.…”

Section: Introductionmentioning

confidence: 99%

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

et al. 2020

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Brewer’s spent grain (BSG) was chemically characterized obtaining 52.1% of carbohydrates, 17.8% protein, 5.9% lipids, 13.5% insoluble lignin and 24.3% of water-soluble extractives. This work has been focused on the study of polyphenol extraction of the extractive fraction by water ultrasound-assisted extraction. Selected extraction conditions were 47 °C and 21.7 mL water/gdry-BSG. The effect of solvent polarity on polyphenol extraction was studied by using ethanol aqueous mixtures, from 20% to 100% ethanol. The kinetics of polyphenol extraction have been fitted to the power law and the Weibull models yielding mean values of the root mean square deviation lower than 7.5%. Extracts have been characterized in terms of quantification of individual phenolic compounds by HPLC-DAD and protein and sugar soluble fractions (glucose, xylose, and arabinose). Polyphenol profile has been compared with other hydrolytic techniques, such as acid, basic and enzymatic hydrolysis, showing that ultrasound was not as effective as basic hydrolysis to release the phenolic acids esterified to the cell wall. A further centrifuge ultrafiltration concentration step was able to yield a retentate enriched in the protein fraction while individual phenolic compounds where mainly transferred to the permeate.

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Section: Introductionmentioning

confidence: 99%

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

et al. 2020

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“…We observed a comparable activity for T. harzianum EM0925 when grown on corn stover without supplementation. These facts illustrate the possibility to develop low-cost degradation enzyme cocktails with high efficiency [2,26]. The high levels of lignocellulolytic enzymes and rapid enzyme production capacity of T. harzianum EM0925 made it a suitable candidate for enzyme cocktail production, since most of the previous studies on developing cost-effective cell wall degrading enzymes have focused on improving the activity of cellulase by adopting genetic operation and screening natural strains so as to obtain high yield of glucose [4,35].…”

Section: Discussionmentioning

confidence: 99%

“…Recently, bio-based industries have developed rapidly, and potential bio-based products including ethanol, aldehydes, organic acids, polyhydric alcohols, and other bio-chemicals and biomaterials have attracted a lot of attention. As the most important intermediate compounds of biological and chemical transformation from biomass, fermentable sugars (mainly glucose and xylose) are crucial for the production of downstream products [1,2]. Due to the structural complexity of lignocellulose, complete enzymatic deconstruction requires the synergistic action of cellulases, hemicellulases, and ligninases [3].…”

Section: Introductionmentioning

confidence: 99%

“…Endoglucanases randomly cleave the internal O-glycosidic bonds and produce glucan chains with different lengths, cellobiohydrolases attack the reducing and non-reducing ends of cellulose to release β-cellobiose, and β-glucosidases hydrolyze the terminal non-reducing β-d-glucosyl residues into glucose. These enzymes can be produced by many fungi (like Aspergillus and Trichoderma) and bacteria (like Cellulomonas and Clostridium) [2,3]. In any case, the rapid and complete conversion of cellulose is the limiting step during the process of bio-refinery [4].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, higher content of cellobiohydrolase in Trichoderma reesei extracellular proteome was detected, which caused the accumulation of cellobiose on account of the deficient amount of β-glucosidase, and then gave rise to severe product feedback inhibition of endoglucanase and cellobiohydrolase [8]. Some researchers found that hemicellulases, oxidoreductases, non-hydrolytic proteins and other auxiliary enzymes synergize to achieve an efficient enzymatic hydrolysis of cellulose [2]. Hemicellulase and pectinase could degrade the non-cellulose polysaccharide that covered cellulose and in turn increase the cellulose hydrolysis efficiency, and this synergistic effect could give rise to the cost reduction in deconstruction of lignocellulose [9].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

Zhang

Yang

Luo

et al. 2020

IJMS

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Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications.

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A bibliometric analysis on potential uses of brewer's spent grains in a biorefinery for the circular economy transition of the beer industry

Ampese

Mussatto²,

Forster‐Carneiro

2021

Biofuels Bioprod Bioref

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This comprehensive review aims to elucidate the state of the art related to research trends regarding the use of brewer's spent grains (BSG), the main solid by-product generated in breweries. Its status, future opportunities, and emerging trends were evaluated by bibliometric analysis, which contributed to clarifying the knowledge and perspectives in the field. In total, 510 documents were obtained from the Web of Science© database in a timespan from 1900 to 2020. VosViewer software and R-bibliometrix package were used to investigate and map the most frequently cited papers, international collaborative networks, keywords, and the main countries and journals associated with this research. The results showed that, globally, 65 countries have been engaged in research related to BSG, and Brazil has been the most productive country, with up to 70 publications. Keyword analysis revealed that the studies on BSG are divided into four main knowledge areas: (i) pretreatment; (ii) nutritional quality; (iii) bioactive compounds, and (iv) pyrolysis. It was also found that a biorefinery can be designed for the complete utilization of BSG to obtain several valuable compounds. In this sense, different technological routes can be applied to produce arabinoxylans, proteins, ferulic acid, xylitol, xylose, lactic acid, butanol, biogas, fertilizer, and ethanol. In conclusion, BSG is a cost-effective and feasible raw material for the sustainable production of bio-based products and bioenergy and its valorization in a biorefinery is a powerful strategy to promote the circular economy transition of the beer industry.

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Enzymatic hydrolysis of brewer’s spent grain to obtain fermentable sugars

Cited by 59 publications

References 36 publications

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

Water Ultrasound-Assisted Extraction of Polyphenol Compounds from Brewer’s Spent Grain: Kinetic Study, Extract Characterization, and Concentration

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

A bibliometric analysis on potential uses of brewer's spent grains in a biorefinery for the circular economy transition of the beer industry

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