Enzymatic hydrolysis of cellulose I is greatly accelerated via its conversion to the cellulose II hydrate form

Wada, Masahisa; Ike, Masakazu; Tokuyasu, Ken

doi:10.1016/j.polymdegradstab.2009.12.014

Cited by 194 publications

(141 citation statements)

References 29 publications

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“…Similar results have been reported for a dilute sulfuric acid pretreatment of hybrid poplar (Sun et al 2014). The high glucose recovery may be primarily a result of the decrease of inter-molecular Hbonds, resulting in the loosening of the cellulose chains, and the weaker hydrophobic interaction by transforming cellulose I to cellulose II (Wada et al 2010). Moreover, the increasing specific surface area and roughness will accelerate the adsorption of cellulase enzymes and subsequently enhance the enzyme attack.…”

Section: Cellulase Hydrolysis Efficiencysupporting

confidence: 81%

Microstructure Properties and Cellulase Hydrolysis Efficiency of Hybrid Pennisetum with [Amim]Cl Pretreatment

et al. 2016

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The complex microstructure of lignocellulosic biomass restricts its conversion into bio-ethanol. In this study, the effects of an ionic liquid (IL) 1-allyl-3-methylimidazolium chloride ([Amim]Cl) pretreatment on the microstructure properties and cellulase hydrolysis efficiency of hybrid Pennisetum (P. americanum × P. purpureum, lignocellulosic biomass) were investigated. After the [Amim]Cl pretreatment, the bonds of lignincarbohydrate complex (LCC) and C=O in xylan were destroyed and the content of inter-molecular H-bonds O(6)H…O(3') decreased by 47.2%, while the content of intra-molecular H-bonds of O(2)H…O(6) and O(3)H…O(5) increased by 9.5% and 47.0%, respectively. The crystallinity and the crystallite size decreased by 20.8% and 42.22%, respectively, and the cellulose crystalline structure changed from cellulose crystalline I to cellulose crystalline II. The specific surface area increased from 0.15 to 10.11 m 2 /g after the [Amim]Cl pretreatment. The glucose recovery increased by 10.3 times after being pretreated with [Amim]Cl, compared with the unpretreated sample.

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Section: Cellulase Hydrolysis Efficiencysupporting

confidence: 81%

Microstructure Properties and Cellulase Hydrolysis Efficiency of Hybrid Pennisetum with [Amim]Cl Pretreatment

et al. 2016

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“…Before saccharification analyses, starch in the cell-wall material was removed by amylase treatment in a similar manner to previous reports (Hattori et al 2012;Park et al 2009;Wada et al 2010). Briefly, the cell-wall material (ca.…”

Section: Starch Removal For Saccharification Analysesmentioning

confidence: 99%

Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency

Yamamura

Noda

Hattori

et al. 2013

Plant Biotechnology

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Lignin is a major component of the secondary cell walls of vascular plants, and an obstacle in the conversion of plant cell wall polysaccharides into biofuels. Erianthus spp. are large gramineous plants of interest as potential energy sources. However, lignocelluloses of Erianthus spp. have not been chemically characterized. In this study, we analysed lignins, related compounds, enzymatic saccharification efficiencies, and minerals in the ash of the inner and outer parts of the internode, leaf blade and leaf sheath of Erianthus arundinaceus. Lignins in four organs consisted of guaiacyl, syringyl, and p-hydroxyphenyl units. The ratios of syringyl to guaiacyl lignins and lignin contents ranged from 0.43 to 0.79 and 20 to 28%, respectively, with values highest in the outer part of the internode. The amounts of ferulic acid were similar (7.3-11.8 mg g −1 dry weight of cell-wall material) in all four organs, while there was more p-coumaric acid in the inner part of the internode (44.7 mg g −1 dry weight of cell-wall material) than in other organs (25.7-28.8 mg g −1 dry weight of cell-wall material). The enzymatic saccharification efficiency (24 h reaction time) of the leaf blade was 21.6%, while those of the other organs ranged from 10.0 to 15.2%. The leaf blade had the highest ash content (17.1%); the main inorganic element was silicon. This paper provides the first fundamental knowledge of E. arundinaceus lignins.

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“…Before saccharification analyses, starch in the cell-wall material was removed by amylase treatment in a similar manner to previous reports (Park et al 2009;Wada et al 2010;Hattori et al in press). Brie y, the cell-wall material (ca.…”

Section: Starch Removal For Saccharification Analysesmentioning

confidence: 99%

“…Acid saccharification e starch-free residue was hydrolysed with a two-step H 2 SO 4 hydrolysis method (Park et al 2009;Wada et al 2010;Hattori et al in press). Brie y, the starch-free residue was incubated in 1 ml of 72% (w/w) H 2 SO 4 at 30°C for 1 h. A er the reaction, 150 µl of the reaction mixture was transferred into fresh 2-ml polypropylene screw-cap tubes containing 1,050 µl of distilled water, and the suspension was heated at 100°C for 2 h. A er the tube was centrifuged at 20,000×g for 3 min, the supernatant was transferred into 14-ml polypropylene round-bottom tubes and neutralized with 1 ml of 25% (w/w) CaCO 3 suspension.…”

Section: Enzymatic Saccharificationmentioning

confidence: 99%

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Retraction: Characterization of lignocellulose of Erianthus ravennae in relation to enzymatic saccharification efficiency

Yamamura

Noda

Hattori

et al. 2012

Plant Biotechnology

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Enzymatic hydrolysis of cellulose I is greatly accelerated via its conversion to the cellulose II hydrate form

Cited by 194 publications

References 29 publications

Microstructure Properties and Cellulase Hydrolysis Efficiency of Hybrid Pennisetum with [Amim]Cl Pretreatment

Microstructure Properties and Cellulase Hydrolysis Efficiency of Hybrid Pennisetum with [Amim]Cl Pretreatment

Characterization of lignocellulose of Erianthus arundinaceus in relation to enzymatic saccharification efficiency

Retraction: Characterization of lignocellulose of Erianthus ravennae in relation to enzymatic saccharification efficiency

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