ENZYMATIC HYDROLYSIS OF THE COENZYME a THIOL ESTERS OF Β-Hydroxypropionic AND Β-Hydroxyisobutyric ACIDS

Rendina, George; Coon, Minor J.

doi:10.1016/s0021-9258(18)64949-x

Cited by 90 publications

(2 citation statements)

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“…HIBCH (3-hydroxyisobutyryl-CoA hydrolase) is an enzyme that catalyzes the conversion of 3-hydroxyisobutyryl-CoA to 3-hydroxyisobutyric acid [27]. It is a key mitochondrial protein required for valine catabolism [28,29]. The metabolite 3-hydroxyisobutyric acid is transformed further to succinyl coenzyme A which is involved in the metabolism of the tricarboxylic acid (TCA) cycle.…”

Section: Discussionmentioning

confidence: 99%

Identification of HIBCH as a Fatty Acid Metabolism-Related Biomarker in Aortic Valve Calcification Using Bioinformatics

Chen

Sun

Xiong

et al. 2022

Oxidative Medicine and Cellular Longevity

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Objective. To identify fatty acid metabolism-related biomarkers of aortic valve calcification (AVC) using bioinformatics and to research the role of immune cell infiltration for AVC. Methods. The AVC dataset was retrieved from the Gene Expression Omnibus database. R package is used for differential expression genes analysis and weighted gene coexpression analysis. The differentially coexpressed genes were identified by the Venn diagram, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of differentially coexpressed genes. Functions closely related to AVC were identified by GO and KEGG enrichment analyses of differentially coexpressed genes. Genes related to fatty acid metabolism were retrieved from the Molecular Signatures Database (MSigDB) database. After removing duplicate genes, least absolute shrinkage and selection operator (LASSO) regression analysis, support vector machine recursive feature elimination (SVM-RFE), and random forest were applied to recognize biomarkers related to fatty acid metabolism in AVC. The CIBERSORT tool was used to analyze infiltration of immune cells in normal and AVC samples. Correlations between biomarkers and immune cells were calculated. Finally, HIBCH-related pathway was predicted by single-gene gene set enrichment analysis (GSEA). Results. 2416 differentially expressed genes and one coexpression module were identified. A total of 1473 differentially coexpressed genes were acquired. GO and KEGG enrichment analyses demonstrated that differentially coexpressed genes were closely related to fatty acid metabolism. LASSO regression analysis, SVM-REF, and random forest revealed that 3-hydroxyisobutyryl-CoA hydrolase (HIBCH) was a biomarker of fatty acid metabolism-related genes in AVC. Significant high levels of memory B cells were found in AVC than normal samples, while activated natural killer (NK) cells were significantly low in AVC than normal samples. A significantly positive relevance was observed between HIBCH and activated NK cells, regulatory T cells, monocytes, naïve B cells, activated dendritic cells, resting memory CD4 T cells, resting NK cells, and CD8 T cells. A significantly negative relevance was observed between HIBCH and activated memory CD4 T cells, memory B cells, neutrophils, gamma delta T cells, M0 macrophages, and plasma cells. The single-gene GSEA results suggest that HIBCH may work through the inhibition of multiple immune-related pathways. Conclusion. HIBCH is closely relevant to immune cell infiltration in AVC and could be applied as a diagnostic marker for AVC.

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Section: Discussionmentioning

confidence: 99%

Identification of HIBCH as a Fatty Acid Metabolism-Related Biomarker in Aortic Valve Calcification Using Bioinformatics

Chen

Sun

Xiong

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Acetyl CoA hydrolase and several other acyl CoA hydrolases have been demonstrated in mammalian tissues (Dekker et al, 1958;Gergely et al, 1952;Porter and Long, 1958;Redina and Coon, 1957;Srere et al, 1959). The probable role of these hydrolytic enzymes in metabolism has been discussed by Srere et al (1959).…”

Section: Discussionmentioning

confidence: 99%

Utilization of volatile fatty acids in ruminants. IV. Relative activities of acetyl CoA synthetase and acetyl CoA hydrolase in mitochondria and intracellular localization of acetyl CoA synthetase

Quraishi¹,

Cook²

1972

J. Agric. Food Chem.

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The relative activity of acetyl CoA hydrolase and acetyl CoA synthetase was investigated in mitochondria from bovine liver, heart, kidney, lung, brain, mammary gland, and skeletal muscle. Acetyl CoA hydrolase activity is high in liver, mammary gland, kidney, and brain. The enzyme is much less active in heart, lung, and skeletal muscle. Hydrolase activity relative to synthetase activity is high in liver and brain. The synthetase activity is much greater than the hydrolase activity in the other tissues. The intracellular localization of acetyl CoA synthetase was determined in bovine heart, kidney, mam-

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The Metabolism of Propionic Acid

Kaziro¹,

Ochoa²

1964

Advances in Enzymology - And Related Areas of Molecular Biology

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ENZYMATIC HYDROLYSIS OF THE COENZYME a THIOL ESTERS OF Β-Hydroxypropionic AND Β-Hydroxyisobutyric ACIDS

Cited by 90 publications

References 31 publications

Identification of HIBCH as a Fatty Acid Metabolism-Related Biomarker in Aortic Valve Calcification Using Bioinformatics

Identification of HIBCH as a Fatty Acid Metabolism-Related Biomarker in Aortic Valve Calcification Using Bioinformatics

Utilization of volatile fatty acids in ruminants. IV. Relative activities of acetyl CoA synthetase and acetyl CoA hydrolase in mitochondria and intracellular localization of acetyl CoA synthetase

The Metabolism of Propionic Acid

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