Enzymatic isolation of human primordial and primary ovarian follicles with Liberase DH: protocol for application in a clinical setting

Is transplantation of cryopreserved ovarian tissue from patients with advanced-stage breast cancer safe? A pilot study

“…For these patients, who run the potential risk of having malignant cells in their cryopreserved ovarian tissue, other options could be of interest, such as follicle culture with in vitro maturation [41,42] or grafting of isolated follicles enzymatically purified from frozen-thawed ovarian tissue [12,13,45].…”

In vitro development of human primordial follicles to preantral stage after vitrification

“…As follicles in earlier stages of development do not contain any small blood vessels, capillaries or white blood cells, grafting would be much safer [57]. In vitro culture has been generally used to evaluate the viability of vitrified ovarian follicles [58].…”

Is transplantation of a few leukemic cells inside an artificial ovary able to induce leukemia in an experimental model?

“…Informed consent was obtained before taking ovarian biopsies from 2 premenopausal women with regular cycles (48 and 49 years of age) undergoing laparoscopic surgery for benign gynecological disease. The tissue was initially processed as previously described [30,31]. Cortical strips, sectioned into pieces of 0.5× 0.5× 1 mm using a MclIwain Tissue Chopper (Mickle Laboratory, UK), were incubated with 0.28Wünsch units/mL Liberase DH (Roche Diagnostics) for 75 min at 37°C with gentle agitation.…”

“…A technique for isolation of human preantral follicles following good manufacturing practice (GMP) conditions was recently optimized by our team with a view to clinical application [30]. In this setting, however, isolated follicles would be retrieved from cryopreserved ovarian tissue that could possibly be contaminated with cancer cells.…”