Enzymatic Mineralization of Silk Scaffolds

Samal, Sangram Keshari; Dash, Mamoni; Declercq, Heidi; Gheysens, Tom; Dendooven, Jolien; Voort, Pascal Van Der; Cornelissen, Ria; Dubruel, Peter; Kaplan, David L.

doi:10.1002/mabi.201300513

Cited by 33 publications

(23 citation statements)

References 40 publications

(75 reference statements)

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“…The infrared measurements were acquired with a Bruker ALPHA FT-IR spectrophotometer (BrukerOptik GmbH, Ettlingen, Germany) in the spectral region of 4000-500 cm −1 [22,23]. This instrument was equipped with an attenuated total reflectance (ATR) sampling accessory.…”

Section: Spectral Characterization Of Nanoparticles: Ft-ir Analysismentioning

confidence: 99%

Designing of Chitosan Derivatives Nanoparticles with Antiangiogenic Effect for Cancer Therapy

et al. 2020

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Angiogenesis is a physiological process involving the growth of new blood vessels, which provides oxygen and required nutrients for the development of various pathological conditions. In a tumor microenvironment, this process upregulates the growth and proliferation of tumor cells, thus any stage of angiogenesis can be a potential target for cancer therapies. In the present study, chitosan and his derivatives have been used to design novel polymer-based nanoparticles. The therapeutic potential of these newly designed nanoparticles has been evaluated. The antioxidant and MTT assays were performed to know the antioxidant properties and their biocompatibility. The in vivo antiangiogenic properties of the nanoparticles were evaluated by using a chick Chorioallantoic Membrane (CAM) model. The obtained results demonstrate that chitosan derivatives-based nanostructures strongly enhance the therapeutic effect compared to chitosan alone, which also correlates with antitumor activity, demonstrated by the in vitro MTT assay on human epithelial cervical Hep-2 tumor cells. This study opens up new direction for the use of the chitosan derivatives-based nanoparticles for designing of antiangiogenic nanostructured materials, for future cancer therapy.While stimulation of angiogenesis in cancer leads to metastasis, the anti-angiogenic therapy proved to be an important approach against tumor growth. In this respect, it is a well-known fact that the consumption of antioxidants can be recommended to achieve the inhibition of angiogenesis or reversal of unwanted cell-cell adherence [1]. Several studies have been conducted to investigate the connection between antioxidants and pathological angiogenesis, thus leading to mixed conclusions from studies that have been associated with a lower total cancer incidence with the use of antioxidants [2], to studies that have highlighted the lack of any benefit of using antioxidants on the incidence of cancer [3]. All these results are, for certain ones, closely related with the type of antioxidant included in the study (alpha tocopherol, beta-carotene, ascorbic acid) and the investigated tumor location and not least the investigated pharmaceutical formulation. In this context, the use of antioxidants in the form of nanoparticles could improve the efficiency of this therapy due to specific surface area of nanostructures [4], thereby ensuring better contact with cells that would increase the chances of pathological angiogenesis inhibition. However, to have more success in cancer therapy, more studies should be conducted in this direction.Chitosan is a natural cationic polymer exhibits three chemical reactive sites including a primary amine and two primary or secondary hydroxyl groups for further modification [5][6][7]. Nano/microparticles coated with chitosan, synthesized through different techniques for encapsulation (chemical, physical and mechanical techniques), are in continuous development, aiming at the entrapment of bioactive substances, enzymes, hormones, antimicrobial agents, vitamins, mi...

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Section: Spectral Characterization Of Nanoparticles: Ft-ir Analysismentioning

confidence: 99%

Designing of Chitosan Derivatives Nanoparticles with Antiangiogenic Effect for Cancer Therapy

et al. 2020

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“…Figure taken from ref. [187] Figure 13. The representation of predicted phospho-sites in silk fibroin.…”

Section: Formulated Products From Processed Rsfmentioning

confidence: 99%

On the Routines of Wild-Type Silk Fibroin Processing Toward Silk-Inspired Materials: A Review

Volkov

Ferreira

Cavaco-Paulo

2015

Macromol. Mater. Eng.

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For years, silk fibroin of a domestic silkworm, Bombyx mori, has been recognized as a valuable material and extensively used. In the last decades, new application fields are emerging for this versatile material. Those final, specific applications of silk dictate the way it has been processed in industry and research. This review focuses on the description of various approaches for silk downstream processing in a laboratory scale, that fall within several categories. The detailed description of workflow possibilities from the naturally found material to a finally formulated product is presented. Considerable attention is given to (bio‐) chemical approaches of silk fibroin transformation, particularly, to its enzyme‐driven modifications. The focus of the current literature survey is exclusively on the methods applied in research and not industry.

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“…Moreover, sericin as hydrophilic natural polymer can be exploited for conjugation with L-asparaginase to overcome the low water solubility of some drugs [8]. New silk biomaterials and biodegradable polymers are also combined to strengthen ceramic bone-scaffold materials made of calcium phosphate [25,26]. The silk biomaterials are folded in complex ways that give it unique properties of both exceptional strength and versatility.…”

Section: Silk Fibre Proteinsmentioning

confidence: 99%

The Mulberry Silkworm—A New Source of Bioactive Proteins

Łochyńska¹

2015

JAST-A

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Abstract:Beyond the commonly known proteins built silk fibres-fibroin and sericin, there are almost 300 bioactive proteins in the silkworm haemolymph. The aim of this work was to present bioactive compounds obtained from the silk fibre and isolated from the body of this insect, which may be used in medical and pharmacological applications. The most important are bioactive proteins. However, the juvenile stages of mulberry silkworm possess other very valuable active substances.

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Enzymatic Mineralization of Silk Scaffolds

Cited by 33 publications

References 40 publications

Designing of Chitosan Derivatives Nanoparticles with Antiangiogenic Effect for Cancer Therapy

Designing of Chitosan Derivatives Nanoparticles with Antiangiogenic Effect for Cancer Therapy

On the Routines of Wild-Type Silk Fibroin Processing Toward Silk-Inspired Materials: A Review

The Mulberry Silkworm—A New Source of Bioactive Proteins

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