Enzymatic recovery of polyester building blocks from polymer blends

Gamerith, Caroline; Zartl, Barbara; Pellis, Alessandro; Guillamot, Frédérique; Marty, Alain; Acero, Enrique Herrero; Guebitz, Georg M.

doi:10.1016/j.procbio.2017.01.004

Cited by 97 publications

(95 citation statements)

References 47 publications

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“…These results show the great importance of finding suitable reaction conditions when specific enzymatic surface functionalizations of the polymer are desired [20,34]. Our findings are perfectly aligned with the previous data collected on PET degradation, where taking particle size and crystallinity of the various PET materials into consideration had a tremendous effect on the enzymatic degradation of the polymer [31]. In addition to High Performance Liquid Chromatography (HPLC) and LC-MS/TOF analysis, scanning electron microscopy analysis of the PEF films surface was performed after 48 h of hydrolysis.…”

Section: Enzymatic Hydrolysis Of Pef Filmssupporting

confidence: 90%

“…As expected, a higher enzymatic activity was observed at the highest reaction temperature, i.e., 65 • C. This is due to the fact that reaction temperatures close to the polymers' T g increase the mobility of the polymer chains, thereby facilitating the enzymatic attack, as also previously reported for PET [31]. As shown in Figure 2, after 24 h of incubation at 65 • C, 3.1, 1.9, 1.6 and 3.4 mM FDCA was released from reactions G (Thc_cut1, Tris-HCl), H (HiC, Tris-HCl), I (Thc_cut1, KPO) and J (Thc_cut1, KPO), respectively.…”

Section: Enzymatic Hydrolysis Of Pef Filmssupporting

confidence: 84%

“…This enzyme was selected from among the variety of hydrolases available in our laboratory since it had previously been reported to hydrolyze both aliphatic poly(lactic acid) and poly(butylene succinate) (PLA, PBS) [13,29] and aromatic (PET, PEF) polyesters [30,31]. Figure 1 shows the time course hydrolysis reaction of the four different powders.…”

Section: Enzymatic Hydrolysis Of Pef Powdersmentioning

confidence: 99%

“…The reaction was studied using two different buffers (0.1 M Tris-HCl pH 7 and 1 M KPO pH 8) and two different reaction temperatures (50 • C and 65 • C). The buffer and temperature selection derived from recent publications describing the efficient hydrolysis of PBS and PET using similar fungal-derived cutinases [13,31,32].…”

Section: Enzymatic Hydrolysis Of Pef Filmsmentioning

confidence: 99%

“…Incubations were conducted in an orbital shaker set at 100 rpm and 50 or 65 • C since good biocatalyst stability and activity over time had been previously reported for these conditions [13,30,31]. Blank reactions were carried out in buffer without addition of the biocatalyst.…”

Section: Poly(ethylene 25-furanoate) Powders and Films Hydrolysismentioning

confidence: 99%

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Enzymatic Degradation of Poly(ethylene 2,5-furanoate) Powders and Amorphous Films

et al. 2017

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Poly(ethylene 2,5-furanoate) (PEF) is arousing great interest as a biobased alternative to plastics like poly(ethylene terephthalate) (PET) due to its wide range of potential applications, such as food and beverage packaging, clothing, and in the car industry. In the present study, the hydrolysis of PEF powders of different molecular masses (M n = 55, M w = 104 kg/mol and M n = 18, M w = 29 kg/mol) and various particle sizes (180 < d and 180 < d < 425 µm) using cutinase 1 from Thermobifida cellulosilytica (Thc_cut1) was studied. Thereby, the effects of molecular mass, particle size and crystallinity on enzymatic hydrolysis were investigated. The results show that particles with lower molecular mass are hydrolyzed faster than those with higher masses, and that the higher the molecular mass, the lower the influence of the particle size on the hydrolysis. Furthermore, cutinases from Humicola insolens (HiC) and Thc_cut1 were compared with regard to their hydrolytic activity on amorphous PEF films (measured as release of 2,5-furandicarboxylic acid (FDCA) and weight loss) in different reaction media (1 M KPO pH 8, 0.1 M Tris-HCl pH 7) and at different temperatures (50 • C and 65 • C). A 100% hydrolysis of the PEF films was achieved after only 72 h of incubation with a HiC in 1 M KPO pH 8 at 65 • C. Moreover, the hydrolysis reaction was monitored by LC/TOF-MS analysis of the released reaction products and by Scanning Electron Microscopy (SEM) examination of the polymer surfaces. Enzymatic hydrolysis of PEF with Thc_cut1 and HiC has potential for use in surface functionalization and recycling purposes.

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Section: Enzymatic Hydrolysis Of Pef Filmssupporting

confidence: 90%

Section: Enzymatic Hydrolysis Of Pef Filmssupporting

confidence: 84%

Section: Enzymatic Hydrolysis Of Pef Powdersmentioning

confidence: 99%

Section: Enzymatic Hydrolysis Of Pef Filmsmentioning

confidence: 99%

Section: Poly(ethylene 25-furanoate) Powders and Films Hydrolysismentioning

confidence: 99%

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Enzymatic Degradation of Poly(ethylene 2,5-furanoate) Powders and Amorphous Films

et al. 2017

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Fundamentals of Polymer Biodegradation Mechanisms

Joseph¹,

Tohidifar²,

Sarver³

et al. 2022

Biodegradable Polymers in the Circular Plastics Economy

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Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

et al. 2019

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Polyethylene terephthalate (PET) is the most important mass‐produced thermoplastic polyester used as a packaging material. Recently, thermophilic polyester hydrolases such as TfCut2 from Thermobifida fusca have emerged as promising biocatalysts for an eco‐friendly PET recycling process. In this study, postconsumer PET food packaging containers are treated with TfCut2 and show weight losses of more than 50% after 96 h of incubation at 70 °C. Differential scanning calorimetry analysis indicates that the high linear degradation rates observed in the first 72 h of incubation is due to the high hydrolysis susceptibility of the mobile amorphous fraction (MAF) of PET. The physical aging process of PET occurring at 70 °C is shown to gradually convert MAF to polymer microstructures with limited accessibility to enzymatic hydrolysis. Analysis of the chain‐length distribution of degraded PET by nuclear magnetic resonance spectroscopy reveals that MAF is rapidly hydrolyzed via a combinatorial exo‐ and endo‐type degradation mechanism whereas the remaining PET microstructures are slowly degraded only by endo‐type chain scission causing no detectable weight loss. Hence, efficient thermostable biocatalysts are required to overcome the competitive physical aging process for the complete degradation of postconsumer PET materials close to the glass transition temperature of PET.

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Enzymatic recovery of polyester building blocks from polymer blends

Cited by 97 publications

References 47 publications

Enzymatic Degradation of Poly(ethylene 2,5-furanoate) Powders and Amorphous Films

Enzymatic Degradation of Poly(ethylene 2,5-furanoate) Powders and Amorphous Films

Fundamentals of Polymer Biodegradation Mechanisms

Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

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