Enzymatic reduction of a less water-soluble ketone in reverse micelles with nadh regeneration

Orlich, Bernhard; Schomaecker, R.

doi:10.1002/(sici)1097-0290(19991105)65:3<357::aid-bit14>3.0.co;2-3

Cited by 29 publications

(16 citation statements)

References 22 publications

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“…The stability of yeast ADH (Fluka) in the microemulsion system has been described in a previous article (Orlich and Schomäcker, 1999a). It did not change significantly by varying the surfactant or water concentration of the microemulsion and the half-life time remains in a range of hours.…”

Section: Stability and Activity Of Different Adh In The Microemulsionmentioning

confidence: 80%

“…Therefore, all measurements including the FDH were made at pH 7. All kinetic data of the cofactor regeneration in the microemulsion with FDH were published earlier (Orlich and Schomäcker, 1999a).…”

Section: The Formate Dehydrogenase In the Microemulsionmentioning

confidence: 99%

“…While lipases, for example, show good stabilities in several organic reaction media (Stamatis et al, 1999), alcohol dehydrogenases are less stable, having half-life times in microemulsions of only hours (Chen et al, 1995;Lee and Biellmann, 1987;Orlich and Schomäcker, 1999a) or even minutes (Samama et al, 1987). The stability depends on the microemulsion parameters and additionally on the reaction components and salts, mainly the water and the surfactant concentration.…”

Section: Introduction Generalmentioning

confidence: 99%

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Stability and activity of alcohol dehydrogenases in W/O‐microemulsions: Enantioselective reduction including cofactor regeneration

Orlich

Berger

Lade

et al. 2000

Biotech & Bioengineering

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Microemulsions provide an interesting alternative to classical methods for the conversion of less water‐soluble substrates by alcohol dehydrogenase, but until now stability and activity were too low for economically useful processes. The activity and stability of the enzymes are dependent on the microemulsion composition, mostly the water and the surfactant concentration. Therefore, it is necessary to know the exact phase behavior of a given microemulsion reaction system and the corresponding enzyme behavior therein. Because of their economic and ecologic suitability polyethoxylated fatty alcohols were investigated concerning their phase behavior and their compatibility with enzymes in ternary mixtures. The phase behavior of Marlipal O13‐60 (C13EO6 in industrial quality)/cyclohexane/water and its effect on the activity and stability of alcohol dehydrogenase from Yeast (YADH) and horse liver (HLADH) and the carbonyl reductase from Candida Parapsilosis (CPCR) is presented in this study. Beside the macroscopic phase behavior of the reaction system, the viscosity of the system indicates structural changes of aggregates in the microemulsion. The changes of the enzyme activities with the composition are discussed on the basis of transitions from reverse micelles to swollen reverse micelles and finally, the transition to the phase separation. The formate dehydrogenase from Candida boidinii was used for the NADH‐regeneration during reduction reactions. While the formate dehydrogenase did not show any kinetic effect on the microemulsion composition, the other enzymes show significant changes of activity and stability varying the water or surfactant concentration of the microemulsion. Under certain conditions, stability could be maintained with HLADH for several weeks. Successful experiments with semi‐batch processes including cofactor regeneration and product separation were performed. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 70: 638–646, 2000.

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Section: Stability and Activity Of Different Adh In The Microemulsionmentioning

confidence: 80%

Section: The Formate Dehydrogenase In the Microemulsionmentioning

confidence: 99%

Section: Introduction Generalmentioning

confidence: 99%

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Stability and activity of alcohol dehydrogenases in W/O‐microemulsions: Enantioselective reduction including cofactor regeneration

Orlich

Berger

Lade

et al. 2000

Biotech & Bioengineering

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“…Organic/aqueous biphasic systems are a bioengineering response to the different solubilities and sensitivities of substrate, product and catalyst. Microemulsions present an intriguing extension of this approach because of the much larger area of mass exchange (Orlich and Schomäcker, 1999). It has been clearly demonstrated that some enzymes work well in supercritical fluids (Section 4.5.2) and in ionic liquids.…”

Section: Process Developments In Microbial and Enzyme Systemsmentioning

confidence: 99%

Biotechnological Flavour Generation

Berger

Krings

Zorn

2010

Food Flavour Technology

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“…Baker's yeast, Saccharomyces cerevisiae, is generally used to prepare enantiomerically pure chiral compounds (Bertau and Burli, 2000;Chin-Joe et al , 2001;Novak et al , 2001;Orlich and Schomaecker, 1999). These reactions mainly correspond to reduction processes.…”

Section: Introductionmentioning

confidence: 99%

Oxidation of a mixture of 2-(R) and 2-(S)-heptanol to 2-heptanone bySaccharomyces cerevisiaein a biphasic system

Cappaert

Larroche

2004

Biocatalysis and Biotransformation

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Enzymatic reduction of a less water-soluble ketone in reverse micelles with nadh regeneration

Cited by 29 publications

References 22 publications

Stability and activity of alcohol dehydrogenases in W/O‐microemulsions: Enantioselective reduction including cofactor regeneration

Stability and activity of alcohol dehydrogenases in W/O‐microemulsions: Enantioselective reduction including cofactor regeneration

Biotechnological Flavour Generation

Oxidation of a mixture of 2-(R) and 2-(S)-heptanol to 2-heptanone bySaccharomyces cerevisiaein a biphasic system

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