Enzymatic release of phosphate in sediments of various origins

Feuillade, M.; Dorioz, Jean Marcel

doi:10.1016/0043-1354(92)90180-c

Cited by 49 publications

(28 citation statements)

References 19 publications

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“…In contrast, Feuillade & Dorioz (1992) detected only small concentrations of phytasehydrolysable P in lake sediments, although this may have been an artefact of the high pH (7.8) used in these experiments (phytase activity decreases to almost zero at pH 7). In the marine environment, White & Miller (1976) demonstrated the presence of small amounts of IP 6 in coastal sediments, whilst Suzumura & Kamatani (1993) detected myo-, chiro-and scyllo-IP 6 in shallow marine sediments from Tokyo Bay.…”

mentioning

confidence: 71%

Inositol phosphates in the environment

Turner¹,

Paphazy

Haygarth³

et al. 2002

Phil. Trans. R. Soc. Lond. B

684

554

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The inositol phosphates are a group of organic phosphorus compounds found widely in the natural environment, but that represent the greatest gap in our understanding of the global phosphorus cycle. They exist as inositols in various states of phosphorylation (bound to between one and six phosphate groups) and isomeric forms (e.g. myo, d-chiro, scyllo, neo), although myo-inositol hexakisphosphate is by far the most prevalent form in nature. In terrestrial environments, inositol phosphates are principally derived from plants and accumulate in soils to become the dominant class of organic phosphorus compounds. Inositol phosphates are also present in large amounts in aquatic environments, where they may contribute to eutrophication. Despite the prevalence of inositol phosphates in the environment, their cycling, mobility and bioavailability are poorly understood. This is largely related to analytical difficulties associated with the extraction, separation and detection of inositol phosphates in environmental samples. This review summarizes the current knowledge of inositol phosphates in the environment and the analytical techniques currently available for their detection in environmental samples. Recent advances in technology, such as the development of suitable chromatographic and capillary electrophoresis separation techniques, should help to elucidate some of the more pertinent questions regarding inositol phosphates in the natural environment.

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mentioning

confidence: 71%

Inositol phosphates in the environment

Turner¹,

Paphazy

Haygarth³

et al. 2002

Phil. Trans. R. Soc. Lond. B

684

554

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“…This fraction was mostly enzymatically hydrolyzable (69-95%). This has important implications for the transfer of P into waterways and the risk of eutrophication because most of the enzymes used in this study are present in natural aquatic ecosystems (21,(28)(29)(30)(31)(32)(33). Figure 3 shows the relative contribution of EHP and DRP to TDP, illustrating the importance of EHP as a potential source of available P, which is usually not considered when measuring the bioavailable P fraction.…”

Section: Dop Speciation In Wastewater Liquorsmentioning

confidence: 99%

“…The EHP fraction represented only 21% of the DOP. However, because most of the enzymes used in this study exist in natural aquatic ecosystems (21,(28)(29)(30)(31)(32)(33) and DOP represented 76% of TDP, the EHP fraction may finally represent a source of available P of similar magnitude to DRP (Figure 3). This result emphasizes the importance of the EHP pool as a source of available P which can feed the water column via diffusion through the sediment-water interface and, hence, potentially increase the risk of eutrophication.…”

Section: Dop Speciation In Wastewater Liquorsmentioning

confidence: 99%

A Protocol to Assess the Enzymatic Release of Dissolved Organic Phosphorus Species in Waters under Environmentally Relevant Conditions

Monbet

McKelvie

Saefumillah

et al. 2007

Environ. Sci. Technol.

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A protocol to assess the potential release of dissolved reactive phosphorus (DRP) by enzymatic hydrolysis of dissolved organic phosphorus (DOP) in waters (sediment porewater and sewage liquors in this study) under environmental conditions is presented. This protocol enables the quantification of different classes of DOP compounds using a variety of phosphatase enzymes, i.e., alkaline phosphatase, phosphodiesterase, and phytase. All experiments were carried out within the pH range of most natural waters, i.e., at neutral (pH 7) or slightly alkaline pH (pH 9). Tri-sodium citrate and sodium dodecyl sulfate (SDS) were used in the assays to prevent interferences due to adsorption processes in the presence of multivalent metallic cations and to minimize protein binding. Applying this protocol revealed that labile phosphate monoesters always represented the largest fraction of enzymatically hydrolyzed P in sewage liquors and sediment porewater. Total enzymatically hydrolyzable P (EHP) represented only 16% of the TDP in the sediment porewater but up to 43% in sewage liquors. Because most of the enzymes used in this study are likely to exist in aquatic ecosystems, the EHP fraction might represent a source of potentially bioavailable P of similar magnitude to DRP.

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“…These enzymes, most notably alkaline phosphatase (APase), 5Ј-nucleotidases and phosphodiesterases, catalyze hydrolysis of phosphoester bonds in phosphomonoesters (ATP, sugar-PO 4 , polyphosphates), and phosphodiesters (RNA, DNA), and belong to the class of enzymes called phosphohydrolases (Walsh, 1979;Ammerman, 1991). Phosphohydrolases are common to natural waters and sediments where they are involved in the degradation of organic matter and regeneration of P i to the water column (Ammerman and Azam, 1985;Feuillade and Dorioz, 1992;Siuda and Güde, 1994;Hoppe and Ulrich, 1999). Hydrolysis of most P org compounds occurs extracellulary, that is, outside of the cell cytoplasm, either in the periplasmic space located between the outer cell wall and cytoplasmic membrane of gram negative bacteria, or completely external to the cell in the surrounding medium.…”

Section: Microbial P Metabolism and Phosphoenzymesmentioning

confidence: 99%

Biogeochemical cycling of phosphorus: Insights from oxygen isotope effects of phosphoenzymes

Blake

O’Neil²,

Surkov³

2005

American Journal of Science

204

252

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ABSTRACT. Geochemical cycling of phosphorus (P) in aquatic environments is carried out almost exclusively by biota and involves reactions that are catalyzed by enzymes. Oxygen isotope effects accompanying phosphoenzymatic reactions have been determined in controlled laboratory experiments in order to elucidate processes underlying biogeochemical cycling of P, and to identify possible reaction pathways for P-compounds in nature. Phosphate oxygen isotope effects are distinct for specific enzymatic reaction mechanisms measured in microbial culture experiments and in cell-free systems. P 16 O 4 is taken up preferentially from inorganic phosphate (P i ) in the growth medium by intact E. coli cells, producing a kinetic fractionation in the extracellular dissolved P i pool. Inorganic pyrophosphatase is the intracellular enzyme that catalyzes the temperature-dependent equilibrium oxygen isotope fractionations between phosphates and water in biological systems, and imprints an equilibrium isotope signature on P i that is turned over or cycled by intact cells. Alkaline phosphatase, a key enzyme involved in extracellular P i regeneration in aquatic systems, catalyzes hydrolysis of phosphomonoesters, reactions that are accompanied by kinetic fractionations and disequilibrium (inheritance) isotope effects in released P i . Comparison of laboratory determined enzyme-specific isotopic fractionations with those observed in microbial culture experiments and in natural aquatic systems, provide new insights into processes controlling P cycling and the relations between P availability and the cycling of N and C. Isotopic signatures associated with specific cellular processes and phosphoenzyme reaction pathways may be useful in assessing P status and for tracing P turnover. Definition of Symbols and Abbreviations

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Enzymatic release of phosphate in sediments of various origins

Cited by 49 publications

References 19 publications

Inositol phosphates in the environment

Inositol phosphates in the environment

A Protocol to Assess the Enzymatic Release of Dissolved Organic Phosphorus Species in Waters under Environmentally Relevant Conditions

Biogeochemical cycling of phosphorus: Insights from oxygen isotope effects of phosphoenzymes

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