Enzymatic Saccharification and L-lactic Acid Fermentation of Corn Stover Pretreated with Liquid Hot Water by Rhizopus oryzae

Kou, Xiaomeng; Yang, Ruijin; Zhao, Jian; Lü, Jie; Liu, Yanjun

doi:10.15376/biores.8.4.4899-4911

Cited by 9 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…LHW is a form of pretreatment that does not use any catalysts or chemicals (Kou et al 2013). During LHW pretreatment, water and acetyl groups within hemicelluloses, which act as acids at elevated temperatures (generally at 160 to 240 o C), can catalyze hydrolysis of hemicelluloses to hemicellulose oligomers and hemicellulose monomers (Cara et al 2007;Wan and Li 2011;Xiao et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Effect of Extractives on Digestibility of Cellulose in Corn Stover with Liquid Hot Water Pretreatment

Li¹,

Yu²,

Sun³

et al. 2015

BioResources

View full text Add to dashboard Cite

Many herbaceous lignocelluloses suitable for ethanol production have high extractives contents, such as some straws and corn stover. The high extractives content might affect pretreatment or enzymatic hydrolysis. In this study, extractives were removed from corn stover, and then extractives-free corn stover and ordinary corn stover were respectively pretreated using a liquid hot water (LHW) method and hydrolyzed to evaluate the effect of extractives on cellulose digestibility. Extractives-free corn stover presented higher cellulose digestibility than ordinary corn stover after the same pretreatment conditions. A total of 87.3% of cellulose was digested in extractives-free corn stover, compared to 71.0% in ordinary corn stover, after pretreatment at 200 °C for 20 min. It is speculated that some water-soluble extractives could buffer H + ions from water and acetic acid during LHW pretreatment process, reducing xylan removal. Another reason for these results might be that some extractives could condense on corn stover after LHW pretreatment, which hinders cellulose hydrolysis.

show abstract

Section: Introductionmentioning

confidence: 99%

Effect of Extractives on Digestibility of Cellulose in Corn Stover with Liquid Hot Water Pretreatment

Li¹,

Yu²,

Sun³

et al. 2015

BioResources

View full text Add to dashboard Cite

show abstract

“…However, plant straw fiber [21,22], kitchen waste [24], and paper sludge [17,19,20] can also be used as substrates. In addition, previous studies have reported on the use of agriculture wastes, such as cotton cellulose [4], corn stover [2,14], and wheat straw [5,16], for lactic acid production.…”

Section: Introductionmentioning

confidence: 99%

Effect of Fermentation Conditions on L-Lactic Acid Production from Soybean Straw Hydrolysate

Wang¹,

Xu²,

Zhang³

et al. 2015

Journal of Microbiology and Biotechnology

View full text Add to dashboard Cite

Four types of straw, namely, soybean, wheat, corn, and rice, were investigated for use in lactic acid production. These straws were mainly composed of cellulose, hemicellulose, and lignin. After pretreatment with ammonia, the cellulose content increased, whereas the hemicellulose and lignin contents decreased. Analytical results also showed that the liquid enzymatic hydrolysates were primarily composed of glucose, xylose, and cellobiose. Preliminary experiments showed that a higher lactic acid concentration could be obtained from the wheat and soybean straw. However, soybean straw was chosen as the substrate for lactic acid production owing to its high protein content. The maximum lactic acid yield (0.8 g/g) and lactic acid productivity (0.61 g/(l/h)) were obtained with an initial reducing sugar concentration of 35 g/l at 30°C when using Lactobacillus casei (10% inoculum) for a 42 h fermentation period. Thus, the experimental results demonstrated the feasibility of using a soybean straw enzymatic hydrolysate as a substrate for lactic acid production.

show abstract

“…Pretreatment is essential to achieving high-efficiency conversion of bioethanol with lignocellulosic biomass. Among the different types of pretreatment, liquid hot water (LHW) pretreatment has been demonstrated to be an effective method for several lignocellulosic biomass types (Kou et al 2013;. After LHW pretreatment, the lignocellulosic biomass is divided into prehydrolysates that contain a large number of fivecarbon sugars and water-insoluble solids (WIS), which mainly consist of glucan and lignin.…”

Section: Introductionmentioning

confidence: 99%

Structural Characterization and Effect on Enzymatic Hydrolysis of Milled Wood Lignin Isolated from Reed Straw and Corn Stover Pretreated with Liquid Hot Water

Li¹,

Liu²,

Lü³

et al. 2016

BioResources

Self Cite

View full text Add to dashboard Cite

aTo assess the possible effects of lignin on enzymatic hydrolysis, the structural characteristics of milled wood lignin (MWL) isolated from reed straw and corn stover pretreated with liquid hot water (LHW) at different severities were investigated. The changes in the chemical structure of the MWL were characterized by gel permeation chromatography, elemental analysis, differential scanning calorimetry, thermogravimetric analysis, Fourier transform infrared spectroscopy, and 1 H and 13 C nuclear magnetic resonance. The results showed that the MWL isolated from reed straw and corn stover pretreated with LHW was more condensed. The nonconjugated and conjugated ketone groups, as well as the ether bonds of the MWL, disappeared after LHW pretreatment, while the phenolic hydroxyl and methoxy groups increased. The carboxyl groups of MWL from reed straw increased after LHW pretreatment, whereas the carboxyl groups of MWL from corn stover decreased. When MWL was added into the enzymatic hydrolysis system, the filter paper activity of cellulase, the protein content, and the conversion of cellulose to glucose all decreased.

show abstract

Enzymatic Saccharification and L-lactic Acid Fermentation of Corn Stover Pretreated with Liquid Hot Water by Rhizopus oryzae

Cited by 9 publications

References 23 publications

Effect of Extractives on Digestibility of Cellulose in Corn Stover with Liquid Hot Water Pretreatment

Effect of Extractives on Digestibility of Cellulose in Corn Stover with Liquid Hot Water Pretreatment

Effect of Fermentation Conditions on L-Lactic Acid Production from Soybean Straw Hydrolysate

Structural Characterization and Effect on Enzymatic Hydrolysis of Milled Wood Lignin Isolated from Reed Straw and Corn Stover Pretreated with Liquid Hot Water

Contact Info

Product

Resources

About