Enzymatic selective synthesis of 1,3-DAG based on deep eutectic solvent acting as substrate and solvent

Abstract

In this study, enzymatic selective esterification of oleic acid with glycerol based on deep eutectic solvent acting as substrate and solvent was studied. As choline chloride (ChCl) or betaine can effectively change the chemical reaction characteristics of glycerol when they are mixed with a certain molar ratio of glycerol, several factors crucial to the lipase catalytic esterification of glycerol with oleic acid was investigated. Results showed that, betaine had more moderate effects than ChCl on the lipase, and water content had an important influence of the esterification and the enzyme selectivity. Significant changes of the glyceride compositions and enzyme selectivity were found in ChCl adding system compared with pure glycerol system; optimum accumulation of DAG especially 1,3-DAG because of the eutectic effect of ChCl was found in this system. Furthermore, in a model 1,3-DAG esterification synthesis system catalyzed by Novozym 435, high content (42.9 mol%) of the 1,3-DAG could be obtained in ChCl adding system within 1 h.

Characteristics of Mango Kernel Fats Extracted from 11 China‐Specific Varieties and Their Typically Fractionated Fractions

“…Triacylglycerol, diacylglycerol and monoacylglycerol levels were analyzed by a high-performance liquid chromatographic system (LC-20AT, Shimadzu, Japan) equipped with a refractive index detector (RID-10A, Shimadzu, Japan) and a silica column (5 µm, 4.6 × 250 mm, Sepax, USA) as described by Zeng et al [20]. Peaks of triacylglycerol, 1,2-diacylglycerol, 1,3-diacylglycerol and monoacylglycerol were identified by comparing the retention times with those of relative standards and the contents were reported in terms of their proportions.…”

Production of High‐Melting Symmetrical Monounsaturated Triacylglycerol‐Rich Fats from Mango Kernel Fat by Acetone Fractionation

“…Fat molecular species, including triacylglycerol, diacylglycerol, monoacylglycerol and free fatty acid (FFA), were separated by a silica column (5 µm, 4.6 × 250 mm, Sepax, USA) and detected by a normal-phase high-performance liquid chromatographic system (LC-20AT, Shimadzu, Japan) equipped with a refractive index detector (RID-10A, Shimadzu, Japan) [23]. The mobile phase was a mixture of n-hexane, isopropanol, and methanoic acid (15:1:0.003, v/v/v) and the flow rate was 1.0 mL/min.…”

Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

“…The deep eutectic solvents (DESs) were prepared according to the method22. The corresponding solid components of the desired DESs in the correct proportion were placed in a 250 mL round-bottom flask.…”