Enzymatic Synthesis and Characterization of Fructooligosaccharides and Novel Maltosylfructosides by Inulosucrase from Lactobacillus gasseri DSM 20604

Abstract

The ability of an inulosucrase (IS) from Lactobacillus gasseri DSM 20604 to synthesize fructooligosaccharides (FOS) and maltosylfructosides (MFOS) in the presence of sucrose and sucrose-maltose mixtures was investigated after optimization of synthesis conditions, including enzyme concentration, temperature, pH, and reaction time. The maximum formation of FOS, which consist of ␤-2,1-linked fructose to sucrose, was 45% (in weight with respect to the initial amount of sucrose) and was obtained after 24 h of reaction at 55°C in the presence of sucrose (300 g liter ؊1 ) and 1.6 U ml ؊1 of IS-25 mM sodium acetate buffer-1 mM CaCl 2 (pH 5.2). The production of MFOS was also studied as a function of the initial ratios of sucrose to maltose (

Highly porous core–shell chitosan beads with superb immobilization efficiency forLactobacillus reuteri121 inulosucrase and production of inulin-type fructooligosaccharides

“…2.4.1.9) is a bacterial fructosyltransferase that can synthesize IFOS from sucrose. Inulosucrase has been identied in only a few bacterial species such as Leuconostoc citreum, 13 Lactobacillus reuteri, 14 Lactobacillus johnsonii, 15 Lactobacillus gasseri, [16][17][18] and Streptomyces viridochromogenes.…”

Novel product specificity toward erlose and panose exhibited by multisite engineered mutants of amylosucrase

“…However, in all cases, maltose acceptor was required in addition to sucrose donor. Production levels around 138, 163, or 61 g L −1 were obtained with levansucrase from B. amyloliquefaciens (using 0.8 M sucrose/0.4 M maltose, Bacillus subtilis (0.7 M sucrose/0.7 M maltose or inulosucrase from Lactobacillus gasseri (0.6 M Sucrose/1.17 M Maltose, respectively). Our process of production compares favorably with these alternative enzymatic processes as the mutants of amylosucrase produce erlose in fairly high amounts from sole sucrose.…”

Therapeutic Spectrum of Nondigestible Oligosaccharides: Overview of Current State and Prospect

“…Wu and others () expressed mutated recombinant β‐galactosidase from Sulfolobus solfataricus P2 in Escherichia coli BL21 (DE3) and obtained GOS. Diez‐Municio and others () produced FOS by using inulosucrase from Lactobacillus gasseri DSM 20604. Kothari and others () used sodium‐alginate‐immobilized glucansucrase from L. mesenteroides in the acceptor reaction with maltose to produce GLOS.…”