Enzymatic synthesis, ligation, and restriction of DNA containing deoxy-4-thiothymidine

Höfer, Bernd; Köster, Hubert

doi:10.1093/nar/9.4.753

Cited by 8 publications

(4 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, under standard conditions, cleavage of SPOl and PBS1 by three and five of these enzymes, respectively, was at least partially inhibited even though these DNAs too have modified T rather than C residues (Tables 1 & 2). These are, therefore, additional examples of the previously detected influence of residues outside of the recognition sequence on the activity of various restriction endonucleases (3,23,32).…”

Section: Discussionmentioning

confidence: 67%

See 1 more Smart Citation

Digestion of highly modified bacteriophage DNA by restriction endonucleases

et al. 1982

View full text Add to dashboard Cite

The ability of thirty Type II restriction endonucleases to cleave five different types of highly modified DNA has been examined. The DNA substrates were derived from relatively large bacteriophage genomes which contain all or most of the cytosine or thymine residues substituted at the 5-position.These substituents were a proton (PBS1 DNA), a hydroxymethyl group (SPO1 DNA), a methyl group (XP12 DNA), a glucosylated hydroxymethyl group (T4 DNA), or a phosphoglucuronated, glucosylated 4,5-dihydroxypentyl group (SP15 DNA). Although PBS1 DNA and SPO1 DNA were digested by most of the enzymes, they were cleaved much more slowly than was normal DNA by many of them. 5-Methylcytosine-rich XP12 DNA and the multiply modified T4 and SP15 DNAs were resistant to most of these endonucleases. The only enzyme that cleaved all five of these DNAs was TaqI, which fragmented them extensively.

show abstract

Section: Discussionmentioning

confidence: 67%

“…TaqI's ability to digest unusually modified DNA had also been seen in its repeated cleavage of phage fd RFI DNA which was ,,90% substituted in one strand with 4-thiothymine residues (32).…”

Section: Resultsmentioning

confidence: 94%

Digestion of highly modified bacteriophage DNA by restriction endonucleases

et al. 1982

View full text Add to dashboard Cite

show abstract

“…A number of physical and biological investigations of mismatch base pairs (1-11) and investigations of base pair interactions where one base is an analog of the standard bases (12)(13)(14)(15)(16)(17)(18) have been reported. The question of whether base pairs with both bases being analogs can have the stability properties of the standard base pairs when incorporated into DNA has not been addressed in the literature.…”

Section: Introductionmentioning

confidence: 99%

The 6-thioguanine/5-methyl-2-pyrimidinone base pair

Rappaport

1988

Nucl Acids Res

View full text Add to dashboard Cite

As part of a program to determine the physical possibility of expanding the number of types of base pairs in DNA, the pairing stabilities of the analog bases 6-thioguanine (GS) and 5-methyl-2-pyrimidinone (TH) in oligodeoxynucleotides were measured. Procedures were developed to synthesize oligodeoxynucleotides with the analog bases. The sequences of the synthesized oligomers were T-C-G-A-C-G-G-X-Y-C-C-G. An enzymatic procedure was developed to measure relative association constants of oligomer pairs with the self complementary reference oligomer, X = A and Y = T, K(T/A) = K. The results were K(C/G) = (5 +/- .5)K, K(TH/GS) = K/(1 +/- .5), K(T/G) = K/(9 +/- 3), K(TH/G) = K/(25 +/- 5), K(C/GS) less than K/30, K(TH/A) less than K/40, K(T/GS) less than K/40, K(C/A) less than K/40. The results with the standard bases are consistent with other methods of measurement. The stability of the base pair GS/TH is approximately the same as the standard base pair A/T.

show abstract

“…Substrates. Isolation of ss and synthesis of ds fd DNA have been described previously (7,8). 1-32 P-ATP was purchased from New England Nuclear.…”

mentioning

confidence: 99%

Primary and secondary structure specificity of the cleavage of ‘single-stranded’ DNA by endo-nuclease HinfI

et al. 1982

Self Cite

View full text Add to dashboard Cite

The interaction of endonuclease Hinf I with single-stranded fd DNA was examined. The sizes of the cleavage products indicate that the enzyme cuts this substrate at the same sequences as double-stranded DNA (GANTC). To determine whether or not the recognition sites in single-stranded DNA have to be present in double-stranded form in order to be cleaved, DNA fragments containing complementary or non-complementary Hinf I sequences were prepared and treated as substrates. The results suggest that completely base-paired recognition sites are necessary for cleavage. Sequences surrounding the Hinf I pentanucleotides significantly modulate the reaction rates.

show abstract

Enzymatic synthesis, ligation, and restriction of DNA containing deoxy-4-thiothymidine

Cited by 8 publications

References 35 publications

Digestion of highly modified bacteriophage DNA by restriction endonucleases

Digestion of highly modified bacteriophage DNA by restriction endonucleases

The 6-thioguanine/5-methyl-2-pyrimidinone base pair

Primary and secondary structure specificity of the cleavage of ‘single-stranded’ DNA by endo-nuclease HinfI

Contact Info

Product

Resources

About