Enzymatic Synthesis of Citrate from Pyruvate and Oxalacetate

Korkes, Seymour; Stern, Joseph R.; Gunsalus, I. C.; Ochoa, Severo

doi:10.1038/166439b0

Cited by 95 publications

(39 citation statements)

References 4 publications

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“…at 3' in a Servall superspeed refrigerated centrifuge. Precipitation in the presence of MnCI, (Korkes, del Campillo, Gunsalus & Ochoa, 1951) removed some impurities, together with some of the DNase activity. Precipitates were formed first at pH 6 by addition of HCI, second at pH 8.5 with added NaOH, and lastly a t about pH 6.8.…”

Section: Bacterial Deoxyribonucleic Acids 525mentioning

confidence: 99%

Studies of Extracellular and Intracellular Bacterial Deoxyribonucleic Acids

Catlin

Cunningham

1958

Journal of General Microbiology

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SUMMARY : Highly polymerized bacterial deoxyribonucleic acids (DNAs) obtained from extracellular and from intracellular locations were analysed. DNAs from two strains (Staphylococcus aureus and S. epidermidis) were of the type having high adenine and thymine contents ; two others (Alcaligenes faecalis and Pseudomonas fluorescens) had a high content of guanine and cytosine. For each micro-organism the composition of extracellular DNA was essentially the same as that of the intracellular DNA. Conditions associated with the extracellular accumulation of DNA in cultures of 3 strains were investigated. S. aureus elaborated a deoxyribonuclease (DNase) which required calcium ion and high pH for activity. In a culture medium of c. pH 6 and insufficient calcium, the DNase was inactive and DNA slime accumulated. In cultures of P . jluorescens a specific ribonuclease-sensitive DNase inhibitor protected slime DNA from depolymerization by culture DNase. In cultures of A . faecalis, also, both a DNA slime and a low concentration of DNase may occur. This enzyme, detected by studying its action against solutions of calf thymus DNA prepared from dried fibres, was activated by various cations within the pH range of cultures. Unlike other DNases, however, it has little capacity to attack the slimelayer DNA, which presumably is more nearly native.

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Section: Bacterial Deoxyribonucleic Acids 525mentioning

confidence: 99%

Studies of Extracellular and Intracellular Bacterial Deoxyribonucleic Acids

Catlin

Cunningham

1958

Journal of General Microbiology

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“…1.1.1.38 (L-malate:NAD oxidoreductase(oxalacetate decarboxylating)) which includes malic enzyme from Lactobacillus arabinosus (Korkes et al, 1950) and the enzyme from dung beetle catharsius (Imbuga and Pearson, 1982). E.C.…”

mentioning

confidence: 99%

pH dependence of the kinetic parameters for oxalacetate decarboxylation and pyruvate reduction reactions catalyzed by malic enzyme

Park¹,

Harris²,

Cook³

1986

Biochemistry

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“…All of the substrates used were of the highest grade commercially available. Crystalline potassium pyruvate (9), LS-isocitrate (17), and sodium glyoxylate monohydrate (15) were prepared in the laboratory. Cytochrome c from horse heart, yeast coenzyme concentrate, glucose-6-phosphate dehydrogenase, and Type III hexokinase from yeast were purchased from the Sigma Chemical Co. Substrates, cofactors, and the particles suspended in the washing medium were placed in the main compartment of chilled Warburg vessels.…”

Section: Methodsmentioning

confidence: 99%