1998
DOI: 10.1006/abio.1998.2781
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Enzymatic Synthesis of ω-Amino-Ceramide: Preparation of a Sensitive Fluorescent Substrate for Ceramidase

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Cited by 35 publications
(22 citation statements)
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“…Precoated Silica Gel 60 TLC plates were obtained from Merck (Whitestation, NJ). , and C12-NBD-Cer was prepared as described elsewhere (17). All other reagents were of the highest purity available.…”
Section: Methodsmentioning
confidence: 99%
“…Precoated Silica Gel 60 TLC plates were obtained from Merck (Whitestation, NJ). , and C12-NBD-Cer was prepared as described elsewhere (17). All other reagents were of the highest purity available.…”
Section: Methodsmentioning
confidence: 99%
“…Anti-myc and anti-GFP antibodies were purchased from Invitrogen Co. Antineutral CDase antibody was raised in a rabbit using the recombinant rat CDase as the antigen (17). C12-NBD-Cer was prepared as described in a previous study (24). HEK293 cells (JCRB9068, established by F. L. Graham) were obtained from the Human Science Research Resource Bank.…”
Section: Methodsmentioning
confidence: 99%
“…The anti-neutral rat CDase antibody was raised in a rabbit using recombinant CDase as the antigen (10). C12-NBD-Cer was prepared as described (21). All other reagents were of the highest purity available.…”
Section: Methodsmentioning
confidence: 99%
“…CDase Assay-CDase activity was measured using C12-NBD-Cer as a substrate as described (18,21). Briefly, 400 pmol (for cell lysates) or 2 nmol (for embryo lysates) of C12-NBD-Cer was incubated at 37°C for 1 h with an appropriate amount of enzyme in 40 l of reaction mix (50 mM Tris-HCl buffer, pH 7.5, containing 0.05% Triton X-100).…”
Section: Methodsmentioning
confidence: 99%