Enzymatic transesterification of waste vegetable oil to produce biodiesel

Lopresto, Catia Giovanna; Naccarato, S.; Albo, L.; Paola, Maria De; Chakraborty, Sudip; Curcio, Stefano; Calabrò, Vincenza

doi:10.1016/j.ecoenv.2015.03.028

Cited by 77 publications

(33 citation statements)

References 20 publications

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“…Figure 1 shows that the highest oil rendement was obtained using n-hexane, at a ratio of 1 :1 (150 mL each), although the quantity of solvent is maximum. Furthermore, a similar case was observed on the methyl acetate solvent, the oil rendement increased by the elevation of effluent amount, till the enhanced oil rendement was small relatively or tends to be constant [9]. However, this research indicated the highest yield of 81.07 %, was obtained using n-hexane, at the solvent effluent to volume ratio of 1:1, and extraction time of three days.…”

Section: Discussionsupporting

confidence: 76%

“…However, before wastewater can be released into an environment, it must first be processed to set quality standards [8]. Furthermore, to overcome the problem of high acid content in the raw material, biodiesel production involves the use of HCl as a catalyst during esterification process and NaOH in subsequent transesterification processes [9]. However, this procedure can further be catalyzed, using alkaline, with high reaction rates, under the low conditions [10].…”

Section: Introductionmentioning

confidence: 99%

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The Utilization of Palm Oil Mill Effluent For Renewable Energy

Nuryanti¹,

Agustina

Sari

2019

IJFAC

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The demand of energy in the world today has increased exponentially, therefore, more efforts have been focused on looking for alternative renewable sources, such as biodiesel, which involves fuel produced from oil of plant, or animal fat. The objective of the research, therefore, was to utilize the palm oil mill effluent (POME) as a raw material in the production of biodiesel, by applying varying solvent, extraction time and the effluent to solvent ratio. Furthermore, the best output of oil rendement of (81.07%), in comparison with the total sample taken, was obtained using n-hexane, at an extraction time of 3 days, and ratio of 1:1. Furthermore, the output possessed a high acid number. Hence, it is considered in the process of esterification and transesterification, required in the production of biodiesel. The HCl catalyst ratio of 1.25% (%v) was used in the esterification process, and the lowest acid number of 2.08 mg-KOH/gr was obtained, followed by the transesterification process, using 1.5% (%w) of NaOH catalyst of the oil weight. Hence, the characteristics of biodiesel produced were in accordance with SNI 04-7182-2006.

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Section: Discussionsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

The Utilization of Palm Oil Mill Effluent For Renewable Energy

Nuryanti¹,

Agustina

Sari

2019

IJFAC

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“…A similar outcome has been reported by Caetano [19]. On the other hand, Lopresto et al [34] reported that the optimum temperature for PcL was 37 °C. The variation of the optimum temperature was varied from 3 °C to 10 °C depend on the lipase and substrate that has been used in the process.…”

Section: Effect Of Lipase Activity Towards Temperaturesupporting

confidence: 82%

Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil

Gusniah

Veny

Hamzah

2020

Bull. Chem. React. Eng. Catal.

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Biodiesel is fatty acid methyl ester that commonly derived from vegetable oils and animal fats that can be produced through enzymatic transesterification using lipase. In this study, three different types of lipase were used, which are Lipase Immobilized Pseudomonas cepacia, PcL, Thermomyces lanuginosus, TLIM, and Candida Antarctica A (recombinant from Aspergillus oryzae), CALA. These lipases were compared based on their activity at different pH (6-10), temperature (30-50 °C), activation energy, and amount of lipase loading for hydrolysis of p-NPA into n-NP. The result indicates that among the lipase used in the study, CALA is the preferable biocatalyst in the hydrolysis of p-NPA due to the minimum energy required and higher enzymatic activity at 20 mg of enzyme loading. PcL and CALA used in the study gave the optimum activity at pH 9 except for TLIM at pH 8 and the optimum temperature at 40 °C. The kinetic data obtained for CALA in this reaction were Km = 57.412 mM and Vm = 70 µM/min. This finding shows that CALA is beneficial biocatalysts for the transesterification process to obtain a higher product with lower activation energy. Copyright © 2020 BCREC Group. All rights reserved

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“…Catalyst plays a significant role for the production of biodiesel with regard to productivity of the process along with its separation. Different researchers used varieties of catalyst for the preparation of alternative fuel using different raw materials [3][4][5][6] . Present authors also prepared biodiesel from Jatropha Curcas oil along with a comparative study between chemical and biocatalytic method 7 .…”

Section: Introductionmentioning

confidence: 99%

Recycling of enzyme- A novel technology for cost effective synthesis of biodiesel

2020

JCBSC

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Recycling of enzyme for the preparation of biodiesel from rice bran oil fatty acid distillate (RBOFAD) is a novel technology along with a cost effective ecofriendly process. Biodiesel has been recognized as future fuel which can be prepared from cheap raw materials in the presence of catalyst enzyme maintaining certain reaction parameters. In our study, Novozyme 40013 (lipase from Candida antarctica) has been used and recycled for this purpose with special emphasis on the productivity of the process. Initially, the process parameters are optimized for the synthesis of biodiesel from RBOFAD and methanol at a definite temperature with a molar ratio of 4:1 (alcohol:RBOFAD) for 180 minutes at 5% concentration of enzyme. After that, enzyme has been successfully recycled twenty times in laboratory scale for biodiesel production by applying the optimized reaction parameters. The main emphasis for recycling of enzyme is to reduce the production cost along with minimization of process hazards. The physical properties of Recycling … Sumit Nandi et al.

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Enzymatic transesterification of waste vegetable oil to produce biodiesel

Cited by 77 publications

References 20 publications

The Utilization of Palm Oil Mill Effluent For Renewable Energy

The Utilization of Palm Oil Mill Effluent For Renewable Energy

Activity and Stability of Immobilized Lipase for Utilization in Transesterification of Waste Cooking Oil

Recycling of enzyme- A novel technology for cost effective synthesis of biodiesel

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