Enzymatische Bestimmung des Gesamt-Cholesterins im Serum

Röschlau, Peter; Bernt, Erich; Gruber, Wolfgang

doi:10.1515/cclm.1974.12.9.403

Cited by 369 publications

(169 citation statements)

References 10 publications

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“…Serum cholesterol and triglycerides were measured by standard hospital laboratory methods [21,22]. A portion of serum was centrifuged at 250 g for 15 rain to prepare platelet-rich plasma.…”

Section: Methodsmentioning

confidence: 99%

Improvement of diabetic control by continuous subcutaneous insulin infusion therapy changes fatty acid composition of serum lipids and erythrocytes in Type 1 (insulin-dependent) diabetes

1986

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Summary. The influence of improved diabetic control on the fatty composition of serum lipids, erythrocytes and platelets was investigated in 24patients with Type 1 (insulin-dependent) diabetes treated for 6 months with either continuous subcutaneous insulin infusion (n = 14) or conventional insulin therapy (n = 10). The groups were matched for age, sex, body mass index, serum lipids, duration of diabetes, glycosylated haemoglobin and insulin dose. Glycaemic control improved, and the contents of dihomogammalinolenic acid and arachidonic acid but not linoleic acid rose significantly (p < 0.05), in serum lipids of patients treated with continuous infusion. No changes were observed in the group treated with insulin injections. Both in serum and erythrocytes the n-6 polyunsaturated fatty acid ratios rose consistently in the patients, with improvement of control regardless of the mode of treatment. Furthermore, the change of HbA1 was negatively correlated with that of arachidonic acid in erythrocytes. No changes were found in the platelet fatty acid compositions. The findings suggest that improved diabetic control enhances the conversion of linoleic acid to arachidonic acid, probably by activating enzymes needed for chain elongation and desaturation.

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“…Serum cholesterol and triglycerides were measured by standard hospital laboratory methods [21,22]. A portion of serum was centrifuged at 250 g for 15 rain to prepare platelet-rich plasma.…”

Section: Methodsmentioning

confidence: 99%

Improvement of diabetic control by continuous subcutaneous insulin infusion therapy changes fatty acid composition of serum lipids and erythrocytes in Type 1 (insulin-dependent) diabetes

1986

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“…Serum concentrations of total cholesterol and triglycerides were measured using enzymatic assays (based on Röschlau et al, 1974;Siedel et al, 1993). HDL-and LDL-cholesterol concentrations were measured using precipitation methods (based on Armstrong and Seidel, 1985;Sugiuchi et al, 1995).…”

Section: Concentrations Of Lipids and Lipoproteins In Serummentioning

confidence: 99%

Similar serum plant sterol responses of human subjects heterozygous for a mutation causing sitosterolemia and controls to diets enriched in plant sterols or stanols

et al. 2007

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Objective: We investigated the serum phytosterol responses of heterozygous relatives of sitosterolemia patients to diets enriched in phytosterols or stanols. Design: Randomized double-blind crossover design. Setting: Muenster, Germany. Subjects: Eight heterozygous and 13 control subjects were recruited. One heterozygote and three controls dropped out. Interventions: Seven heterozygotes and 10 controls received daily portions of margarine containing 2 g of plant sterols, 2 g of stanols or a control margarine for 6 weeks each in a randomized order. These phases were intercepted by wash-out periods of 6 weeks each. Results: Compared to the control period, serum phytosterol concentrations increased overall by more than 20% when subjects consumed the plant sterol margarine (F (1,15) ¼ 8.719, P ¼ 0.01), with no significant difference between heterozygotes (mean þ 14.5 (s.d. 17.2) mmol/l, þ 23.0%) and controls ( þ 4.9 (9.9) mmol/l, þ 20.5%; F (1,15) ¼ 2.168, P ¼ 0.162), but decreased when subjects consumed the stanol-enriched margarine (F (1,15) ¼ 12.124, P ¼ 0.003), again to a similar extent in heterozygotes (À34.2 (41.2) mmol/l, À54.2%) and controls (À12.2 (9.2) mmol/l, À50.6%; F (1,15) ¼ 2.729, P ¼ 0.119). The lowest total serum concentrations of cholesterol and phytosterols were seen after the diet enriched in stanols. Serum stanol concentrations increased on this diet, but on a very low level and never exceeded 0.05% of serum cholesterol levels in any subject. Conclusions: Serum phytosterol concentrations increased only moderately in heterozygotes consuming a diet enriched in phytosterols, indicating that they retained considerable capacity to excrete phytosterols even at higher intakes. Sponsorship: Supported by a grant of the Stifterverband für die Deutsche Wissenschaft (project number TS022/12372/2002) to MK.

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“…TC and TG were assayed using enzyme of age, sex, smoking habits and BMI, while the avermethods, with kits supplied by Boehringer age systolic and diastolic BP values were higher in (Mannheim, Germany). 43,44 The HDL-C concenthe patients with essential hypertension. tration was measured after precipitation of the lipo- Table 2, on the other hand, shows the plasma conproteins containing Apo B with phosphotungstic acid and subsequent enzymatic assaying of choles- Table 2 Apolipoprotein profile of normotensives and hypertenterol.…”

Section: Determination Of the Apolipoprotein Profilementioning

confidence: 99%

Lp(a) in hypertensive patients

et al. 1998

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However, higher plasma concentrations of Lp(a), albeit within the normal range, could be an independent risk women) were studied and compared with 89 controls (36 men and 53 women) matched in terms of age, sex, body factor for atherosclerosis, and could contribute towards increasing the incidence of cardiovascular disease in mass index (BMI) and smoking habits. It was found that the hypertensive patients had higher plasma concenpeople with essential arterial hypertension.

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Enzymatische Bestimmung des Gesamt-Cholesterins im Serum

Cited by 369 publications

References 10 publications

Improvement of diabetic control by continuous subcutaneous insulin infusion therapy changes fatty acid composition of serum lipids and erythrocytes in Type 1 (insulin-dependent) diabetes

Improvement of diabetic control by continuous subcutaneous insulin infusion therapy changes fatty acid composition of serum lipids and erythrocytes in Type 1 (insulin-dependent) diabetes

Similar serum plant sterol responses of human subjects heterozygous for a mutation causing sitosterolemia and controls to diets enriched in plant sterols or stanols

Lp(a) in hypertensive patients

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