Enzyme activities and decolourization of single and mixed azo dyes by the white-rot fungus Phanerochaete chrysosporium

Singh, Sawraj; Pakshirajan, Kannan

doi:10.1016/j.ibiod.2009.11.003

Cited by 48 publications

(29 citation statements)

References 19 publications

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“…Manganese peroxidase and laccase have previously been linked directly to the degradation of aromatic compounds, including decoloration of synthetic dyes by white rot fungi [2,11,18,26]. However, dyes containing highly recalcitrant structures could limit the decoloration ability of the fungus since they require higher redox potential of a catalyst to initiate electron distribution in a degradation mechanism [6,16,17].…”

Section: Screening For Lignin-modifying Enzymesmentioning

confidence: 99%

“…Though similar in gross morphology, they have diverse anatomical, physiological, and molecular biological characteristics [15]. Phanerochaete chrysosporium is the most extensively studied white rot fungus due to its effi ciency in attacking a range of condensed aromatic pollutants and lignin [16][17][18][19]. Broader studies of this resupinate group address their biodegradative capabilities [10], and specifi c focus has centered on Dichomitus squalens, Irpex lacteus, and Bjerkandera adusta [2,[20][21][22].…”

Section: Introductionmentioning

confidence: 99%

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Biological Treatment of Reactive Black 5 by Resupinate White Rot Fungus Phanerochaete sordida PBU 0057

Permpornsakul¹,

Prasongsuk²,

Lotrakul³

et al. 2016

Pol. J. Environ. Stud.

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Resupinate white rot fungi were isolated from across Thailand (seven provinces) and evaluated for their ability to degrade the recalcitrant and toxic dye reactive black 5 (RB5). Seven of the 13 isolates decolorized RB5 (100 mg/L) within three days (greater than 50%). One isolate, Phanerochaete sordida PBU 0057, yielded complete decoloration within three days. It was active under environmental conditions in which waste dyes are discharged. The degradation of RB5 by P. sordida PBU 0057 was confi rmed by spectrophotometric and HPLC analyses. The degradation was associated with the activities of laccase and lignin peroxidase. Manganese peroxidase was additionally active in the comparative control strain of P. chrysosporium. The degradation dye products produced by P. sordida PBU 0057 were not phytotoxic towards Phaseolus vulgaris, Sorghum bipolar, and Zea mays. Overall, P. sordida PBU 0057 showed somewhat greater dye-degradative activities when compared to the control reference strain P. chrysosporium ATCC 24725 under practical environmental conditions. P. sordida PBU 0057 has potential for application in the biological detoxifi cation treatment of waste dye residues.

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Section: Screening For Lignin-modifying Enzymesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biological Treatment of Reactive Black 5 by Resupinate White Rot Fungus Phanerochaete sordida PBU 0057

Permpornsakul¹,

Prasongsuk²,

Lotrakul³

et al. 2016

Pol. J. Environ. Stud.

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“…Most of the white rot fungi are wood rotting basidiomycetes, are capable of decaying lignin based polymers and several variable recalcitrant environmental pollutants due to its inherent ability to secrete cellulolytic and lignolytic enzymes such as lignin peroxidase, manganese peroxidase and laccases which aid the decaying process of dyes and inorganic pollutants (Ogbo et al, 2006). Lignin peroxidase, polyphenol oxidase and laccases produced by white rot fungi, degrade many aromatic compounds (Singh and Pakshirajan, 2010).…”

Section: Introductionmentioning

confidence: 99%

Extracellular secretion of lignocellulolytic enzymes by diversewhite rot asidiomycetes fungi

Thiribhuvanamala¹,

Kalaiselvi²,

Parthasarathy³

et al. 2017

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Rich source of biota includes several white rot fungi, were reported in Western Ghats regions of Tamil Nadu, India. The effective identification of white rot fungi and their lignocellulolytic enzymes play a crucial role for degradation of agricultural and industrial wastes to maintain su sta ina ble ecosystem. T hus, the present study was conducted to assess the secretion of lignocellulolytic enzymes of diverse white rot fungi, collected from Western Ghats regions of Tamil Nadu, comprising of Kotagiri and Ooty of the Nilgiri district, Siruvani and Anaikati of Coimbatore district. A total of 16 white rot fungi belonging to 6 genera, viz., Pleurotus spp., Lentinus spp., Ganoderma spp., Trametes spp., Pycnoporus spp. and Schizophyllum spp. were collected and identified through morphological and molecular methods. The activity of cellulase enzyme was prominently visible in Pleurotus pulmonarius, P. sajar-caju, Pycnoporus sanguineus, P. ostreatus, Ganoderma gibbosum and Schizophyllum commune and hemicellulolytic activity was maximum in Trametes ijubarskii, Lentinus crinitus, P. sanguineus and G. gibbosum . The quantitative and qualitative screening for major lignolytic enzymes, viz., laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP) revealed significant levels of Lac secretion when compared to LiP and MnP by P. pulmonarius, P. sajar-caju and Schizophyllum commune. The syringaldazine well test further confirmed the secretion of laccase as major enzyme followed by LiP and MnP. Also the Lac and LiP activity was maximum at pH of 4.5 and MnP activity at pH of 5.0 by P. pulmonarius, P. sajar caju and S. commune. Temperature of 30°C induced maximum secretion of Lac, LiP and MnP by these three fungi. A significant level of Lac was observed on 4 th day after inoculation while LiP and MnP was maximum on 7 th and 8 th day for P. pulmonarius and P. sajar-caju. Whereas, S. commune secreted significant levels of Lac on 10 th to 11 th da y, while LiP and MnP on 6 th and 7 th da y. This study paves the way for u se of P. pulmonarius, P. sajar-caju and S. commune for biodegradation and bioconversion of agrowastes.

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“…The absorbance was measured at the wavelength corresponding to the maximum absorption for each dye. The discoloration rate was calculated according to the equation: % discoloration = (Ai-Af) / Ai * 100, where Ai is the initial absorbance at λmax and Af is the absorbance after incubation period [28]. All the calculations were verified using a calibration curve.…”

Section: Spectrophotometric Studiesmentioning

confidence: 99%

The use of heavy metals in mycoremediation of synthetic dyes

2014

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Synthetic dyes represent a major class of toxic pollutants that are resistant to biological degradation, and heavy metals play a special role in this process. In this study, two isolates of lignicolous macromycetes, Lenzites betulina as well as another species less frequently studied, Trametes gibbosa, were tested in order to assess their remediation potential against three different synthetic dyes under specific conditions. The effect of heavy metal ions in the discoloration of synthetic dyes process, and the optimal concentration of manganese ions necessary were evaluated. The dyes’ discoloration efficiency of the fresh isolates were compared to the isolates maintained by refrigeration, isolates that were repeatedly sub-cultivated and isolates that were previously grown on dye-supplemented media, and then were assessed. The discoloration process was evaluated in liquid nutrient media, 10 replicates were used for each working version. Evaluation of discoloration rate was obtained by using a UV-VIS spectrometer. The results were interpreted statistically by the Kruskal-Wallis test and by use of a new complex method, Multiple Factor Analysis. The fresh isolates showed the highest discoloration capacity while the isolates previously grown on the dye-supplemented media presented a low discoloration rate. Manganese ions gave a positive effect on enzyme induction while copper and cobalt ions inhibited the process.

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Enzyme activities and decolourization of single and mixed azo dyes by the white-rot fungus Phanerochaete chrysosporium

Cited by 48 publications

References 19 publications

Biological Treatment of Reactive Black 5 by Resupinate White Rot Fungus Phanerochaete sordida PBU 0057

Biological Treatment of Reactive Black 5 by Resupinate White Rot Fungus Phanerochaete sordida PBU 0057

Extracellular secretion of lignocellulolytic enzymes by diversewhite rot asidiomycetes fungi

The use of heavy metals in mycoremediation of synthetic dyes

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