Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia

Msarah, Marwan; Ibrahim, Izyanti; Aqma, Wan Syaidatul

doi:10.1063/1.5027985

AIP Conference Proceedings

2018

DOI: 10.1063/1.5027985

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Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia

Marwan Msarah¹,

Izyanti Ibrahim²,

Wan Syaidatul Aqma³

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Cited by 2 publications

References 12 publications

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Production of Enzymes in Predominant Thermophilic Fungi Available From Organic Substrates

Birajdar

Bhale

2021

int.jour.sci.res.mana.

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Present investigation describes that the study site comes under Aurangabad Division Maharashtra and it falls in Deccan Plateau Zone of India. It was collected different types of organic substrates viz. vermiompost, poultary manure, baggase, farm yard manure (FYM), soil, Ash etc. Isolated thermophilic predominant fungi thermophilic fungi viz.Aspergillus niger, Mucor mucedo,Humicola insolens,Trichoderma harzianum,T. viride,Penicillium duponti,Fusarium oxysporun and Chaetomium thermophilum were carried out for the production of enzymes. Isolated predominant thermophilic fungi were evaluated on different types of enzymes. Among tested thermophilic fungi, the highest ativity was observed in C. thermophilium (20mm) followed by T. harzianum (19.50mm) In lipase, M. mucedo (15.40mm) was found maximum followed by F. oxysporun. Cellulase activity was found highest in A. nige (25mm) followed by others. In case of xylanase, catalase, peroxidase and esterase activities were found maximum, minimum and medium even negative in some fungi. Maximum pectinase activity was detected from H. insolens (52.26 @ 0 min) and (74.25 @ 10 min) and in case of M. mucedo, F. oxysporun and C. thermophilium was found most extreme while least in A. niger (30.12) and P. duponti (33.47) @ 0 minute. Key words: Organic Substrates, Thermophilic Fungi, Enzymes

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Production of Enzymes in Predominant Thermophilic Fungi Available From Organic Substrates

Birajdar

Bhale

2021

int.jour.sci.res.mana.

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Isolation and Characterisation of Thermophilic Bacillus licheniformis SUNGC2 as Producer of α-Amylase from Malaysian Hot Spring

Msarah¹,

Firdose²,

Ibrahim³

et al. 2020

JST

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Screening of new source of novel and industrially useful enzymes is a key research pursuit in enzyme biotechnology. The study aims to report the characteristics of novel thermophilic microorganisms isolated from Sungai Klah (SK) Hot Spring, Perak, Malaysia, that can produce α-amylase. The morphological and biochemical properties were examined for SUNGC2 sample. The isolate was further screened for amylase, followed by 16S rRNA and analytical profile index (API) test. This isolate was further subjected to pH optimisation for α-amylase production. It was found that SUNGC2 was an α-amylase producer and was identified as Bacillus licheniformis SUNGC2 with NCBI accession numbers MH062901. The enzyme was found to exhibit an optimum temperature of 50°C and a pH of 7.0. The relative activity of the enzyme was obtained based on the improvement of the culture conditions. The highest amount of amylase production was 24.65 U/mL at pH 7.0, consecutively the growth was also highest at pH 7.0 with a 9.45-fold increase in specific activity by ammonium phosphate precipitation of 80% (w/v). The results showed that the bacteria isolated from the hot spring are a significant source of thermophilic enzymes that are highly promising in biotechnology.

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Enzyme activity screening of thermophilic bacteria isolated from Dusun Tua Hot Spring, Malaysia

Cited by 2 publications

References 12 publications

Production of Enzymes in Predominant Thermophilic Fungi Available From Organic Substrates

Production of Enzymes in Predominant Thermophilic Fungi Available From Organic Substrates

Isolation and Characterisation of Thermophilic Bacillus licheniformis SUNGC2 as Producer of α-Amylase from Malaysian Hot Spring

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