Enzyme-assisted Aqueous Extraction of Oil from Rice Germ and its Physicochemical Properties and Antioxidant Activity

Wang, Hong; Geng, Haoyuan; Tang, Honglin; Wang, Liqi; Yu, Dianyu; Wang, Junguo; Song, Yuqing

doi:10.5650/jos.ess19060

Cited by 7 publications

(6 citation statements)

References 36 publications

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“…Liu et al (2019) evaluated the action of the enzymes protease, cellulase, pectinase and hemilcellulase in the extraction of oil from Sapindus mukorossi and obtained the highest YO with protease (∼65%) followed by cellulase (∼61%), while the other enzymes had yield below 50%. In the extraction of pecan oil, Polmann et al (2019) obtained YO with Alcalase® 2.4L FG enzyme (47.3%) greater than with Celluclast® 1.5L enzyme (23.8%), which was also reported by Wang et al (2019) in the extraction of oil from rice germ. Despite the different oleaginous matrices used in the reported works, the protease enzyme proved to be more efficient compared to cellulase.…”

Section: Oil Yieldsupporting

confidence: 60%

Sunflower seed oil: enzymatic aqueous extraction, oil recovery by green solvent and chemical composition

Aquino,

Stevanato,

Raspe

et al. 2023

RMIQ

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Section: Oil Yieldsupporting

confidence: 60%

Sunflower seed oil: enzymatic aqueous extraction, oil recovery by green solvent and chemical composition

Aquino,

Stevanato,

Raspe

et al. 2023

RMIQ

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“…Nonetheless, the raise of incubation time continuously would diminish the cordycepin content. It is proposed that too long incubation time could remarkably create a depletion of cordycepin or product inhibition of enzymes [33] . As the fine powder of material was sediment in the bottom of extraction equipment, this created a fixed extraction bed during a longer period of incubation without agitation.…”

Section: Resultsmentioning

confidence: 99%

“…It is proposed that too long incubation time could remarkably create a depletion of cordycepin or product inhibition of enzymes. [33] As the fine powder of material was sediment in the bottom of extraction equipment, this created a fixed extraction bed during a longer period of incubation without agitation. Therefore, it is difficult for cordycepin to get into contact with solvents.…”

Section: Effect Of Incubation Timementioning

confidence: 99%

Integrating of Aqueous Enzymolysis with Ethanolic Ultrasonication to Ameliorate Cordycepin Content from Vietnamese Cordyceps militaris and Biological Analysis of Extracts

Thanh

Dat

et al. 2021

ChemistrySelect

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In this study, the employment of aqueous enzymolysis using alcalase with ethanolic ultrasonication extraction method was investigated. The cordycepin content was analyzed by using reversed‐phase high‐performance liquid chromatography with a diode array detector. The result showed that the highest cordycepin content of 0.4365±0.0309 mg/g could be obtained under extraction conditions including total enzyme volume of 240 μL, incubation time of 50 min, incubation temperature of 55 °C, and ultrasonic power of 840 W. The comparative studies of antioxidant activities of the extract were determined in terms of the DPPH free radical scavenging assay, ferric ion reducing power assay, and H2O2 scavenging assay. Additionally, the MTT assay was conducted to evaluate the anticancer activities of the extract, illustrating the cytotoxic property against Hep‐G2 cancer cells with the half‐maximal inhibitory concentration (IC50) value of 12.20±0.03 μg/mL compared to that of 0.36±0.03 μg/mL for ellipticine, MCF‐7 cancer cells with the IC50 value of 11.60±0.09 μg/mL compared to that of 0.51±0.04 μg/mL for ellipticine, and Lu cancer cells with the IC50 value of 34.50±0.25 μg/mL compared to that of 0.32±0.03 μg/mL for ellipticine.

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“…Palmitic acid, a long chain saturated fatty acid, is widely distributed in plants, including palm fruit [1,2], maize grain [3], and other cereal seeds [4,5]. In animals and humans, overloading of palmitic acid in non-fat tissues leads to lipotoxicity and diseases [6,7].…”

Section: Introductionmentioning

confidence: 99%

Loss of p53 Sensitizes Cells to Palmitic Acid-Induced Apoptosis by Reactive Oxygen Species Accumulation

Luo

Zhang

et al. 2019

IJMS

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Palmitic acid, the most common saturated free fatty acid, can lead to lipotoxicity and apoptosis when overloaded in non-fat cells. Palmitic acid accumulation can induce pancreatic β-cell dysfunction and cardiac myocyte apoptosis. Under various cellular stresses, the activation of p53 signaling can lead to cell cycle arrest, DNA repair, senescence, or apoptosis, depending on the severity/type of stress. Nonetheless, the precise role of p53 in lipotoxicity induced by palmitic acid is not clear. Here, our results show that palmitic acid induces p53 activation in a dose-and time-dependent manner. Furthermore, loss of p53 makes cells sensitive to palmitic acid-induced apoptosis. These results were demonstrated in human colon carcinoma cells (HCT116) and primary mouse embryo fibroblasts (MEF) through analysis of DNA fragmentation, flow cytometry, colony formation, and Western blots. In the HCT116 p53 −/− cell line, palmitic acid induced greater reactive oxygen species formation compared to the p53 +/+ cell line. The reactive oxygen species (ROS) scavengers N-acetyl cysteine (NAC) and reduced glutathione (GSH) partially attenuated apoptosis in the HCT116 p53 −/− cell line but had no obvious effect on the p53 +/+ cell line. Furthermore, p53 induced the expression of its downstream target genes, p21 and Sesn2, in response to ROS induced by palmitic acid. Loss of p21 also leads to more palmitic acid-induced cell apoptosis in the HCT116 cell line compared with HCT116 p53 +/+ and HCT116 p53 −/− . In a mouse model of obesity, glucose tolerance test assays showed higher glucose levels in p53 −/− mice that received a high fat diet compared to wild type mice that received the same diet. There were no obvious differences between p53 −/− and p53 +/+ mice that received a regular diet. We conclude that p53 may provide some protection against palmitic acid-induced apoptosis in cells by targeting its downstream genes in response to this stress.

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Enzyme-assisted Aqueous Extraction of Oil from Rice Germ and its Physicochemical Properties and Antioxidant Activity

Cited by 7 publications

References 36 publications

Sunflower seed oil: enzymatic aqueous extraction, oil recovery by green solvent and chemical composition

Sunflower seed oil: enzymatic aqueous extraction, oil recovery by green solvent and chemical composition

Integrating of Aqueous Enzymolysis with Ethanolic Ultrasonication to Ameliorate Cordycepin Content from Vietnamese Cordyceps militaris and Biological Analysis of Extracts

Loss of p53 Sensitizes Cells to Palmitic Acid-Induced Apoptosis by Reactive Oxygen Species Accumulation

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