Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides

Jin, Guojie; Yang, Fan; Hu, Cui-Min; Shen, Hongwei; Zhao, Zongbao K.

doi:10.1016/j.biortech.2012.01.152

Cited by 118 publications

(80 citation statements)

References 27 publications

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“…While acetic acid has no potential as fuel, some organisms such as oleaginous yeasts are able to convert acetic acid and other volatile fatty acids (VFAs) into lipids, which could be used as biodiesel [131,132]. A process scheme has been proposed combining such a fermentation process with a CO 2 fermentation process [133].…”

Section: Acetate Producersmentioning

confidence: 99%

Commercial Biomass Syngas Fermentation

2012

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Abstract:The use of gas fermentation for the production of low carbon biofuels such as ethanol or butanol from lignocellulosic biomass is an area currently undergoing intensive research and development, with the first commercial units expected to commence operation in the near future. In this process, biomass is first converted into carbon monoxide (CO) and hydrogen (H 2 )-rich synthesis gas (syngas) via gasification, and subsequently fermented to hydrocarbons by acetogenic bacteria. Several studies have been performed over the last few years to optimise both biomass gasification and syngas fermentation with significant progress being reported in both areas. While challenges associated with the scale-up and operation of this novel process remain, this strategy offers numerous advantages compared with established fermentation and purely thermochemical approaches to biofuel production in terms of feedstock flexibility and production cost. In recent times, metabolic engineering and synthetic biology techniques have been applied to gas fermenting organisms, paving the way for gases to be used as the feedstock for the commercial production of increasingly energy dense fuels and more valuable chemicals.

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Section: Acetate Producersmentioning

confidence: 99%

Commercial Biomass Syngas Fermentation

2012

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“…Extraction with intact and enzymatically treated cells resulted in very low lipid yields (below 6% w/w for both species). Jin et al [18] also reported low lipid extraction yields for R. toruloides with hexane. Sheng et al [39] attributed the low efficiency of hexane to its polarity being lower than that of chloroform and to its inability to damage the cell wall.…”

Section: Lipid Extraction From Intact and Pretreated R Toruloides Anmentioning

confidence: 99%

“…A large variety of solvents have been described for the extraction of SCOs; these include hexane alone [18,21] or mixed with isopropanol [21,22] and combinations of methanol and chloroform, as in the Pedersen [16] and the classical methods of Folch et al [23] and Bligh and Dyer [24]. An efficient extraction requires the solvent to fully penetrate the cell mass and have a polarity similar to that of the target compounds [11].…”

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confidence: 99%

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Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

et al. 2015

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Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters OPEN ACCESSEnergies 2015, 8 5041 (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.

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“…Since cell wall and membrane present in algae are formidable barriers to permeation by extraction solvents, cells have to be disrupted prior to extraction [11], which enhances oil recovery. Methods of cell wall disruption and extracting solvents decide the efficiency of oil extraction from microalgae [11].…”

Section: Introductionmentioning

confidence: 99%

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

Surendhiran

Mani

2014

ISRN Chemical Engineering

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Microalga is one of the most compelling microbial biomasses for biodiesel production. Various pretreatment processes, namely, enzyme treatment, lysis by acid, ultrasonicator, microwaves, autoclave, and 40% NaCl, for nitrogen replete and depleted algal cultures of Nannochloropsis oculata had been carried out to check the most feasible and effective technique to disrupt cells for procuring lipids, for which concentrations were determined. Fatty acid composition, essential functional groups, and cell disruption were analyzed by GC-MS, FT-IR Spectroscopy, and Nile Red fluorescent microscopy, respectively. The present investigation showed that lipid yield was higher in nitrogen depleted cells than that in normally nourished cells. GC-MS revealed the presence of major fatty acids-palmitic, oleic, stearic, arachidic, lauric, and linoleic acids. Highest efficiency was found when cells were pretreated using acid for 3 h. The lipid content was calculated as 33.18% and 54.26% for nitrogen rich cells and nitrogen starved cells, respectively. This work thus aided in identifying the most eligible pretreatment process to avail lipids from cells, to convert them to eco-friendly and nonpolluting biodiesel.

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Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides

Cited by 118 publications

References 27 publications

Commercial Biomass Syngas Fermentation

Commercial Biomass Syngas Fermentation

Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

Effect of Various Pretreatment for Extracting Intracellular Lipid fromNannochloropsis oculataunder Nitrogen Replete and Depleted Conditions

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