Enzyme‐Assisted Extraction of Triterpenoid Saponins from Pseuderanthemum palatiferum (Nees) Radlk. Dry Leaf Powder and Bioactivities Examination of Extracts

Abstract

Triterpenoid saponins were extracted from of Pseuderanthemum palatiferum (Nees) Radlk. dry leaf powder by using enzyme‐assisted extraction method. Effects of single factors on total saponins content (TSC) were investigated. Then, a full factorial design with two levels was conducted to screen the main factors affecting TSC. Two main factors as extraction temperature and time were determined. Response Surface Methodology with central composite design was used to study simultaneous effects of these factors on TSC. Results showed that the optimum conditions were 54.9 °C and 80.8 min. The extract under these conditions was tested with bioactivities. The extract had the haemolytic index of 256.74 mL, anti‐inflammatory activity with IC20 of 0.56 μg/mL, anti‐oxidation activity with IC50 values of DPPH, ABTS, and hydroxyl of 61.47, 1.42, and 30.33 μg/mL, respectively, and cytotoxicity on cancer cell line HepG2. Accordingly, triterpenoid saponins from Pseuderanthemum palatiferum (Nees) Radlk. could be considered as a valuable constituent.

Chemical composition and cellulase-assisted extraction of total saponins from Gomphrena celosioides Mart.

“…In the present study, an extraction temperature of 60EC was selected for all the subsequent experiments (determination of the extraction time). The optimal extraction temperature determined in the present study was slightly higher than that reported by Huy et al (2019) who extracted triterpenoid saponins from the dried root and leaf of Pseuderanthemum palatiferum (Nees) Radlk at 55EC by viscozyme-assisted extraction (viscozyme is a multienzyme complex containing a wide range of carbohydrases, including arabanase, cellulase, β-glucanase, hemicellulase, and xylanase). In addition, the optimal extraction temperature determined in the present study was also significantly higher than that reported by Pan and Wu (2014), who used the CAE method to extract polysaccharides from garlic at 45EC.…”

Application of ultrasound‐assisted enzymatic extraction to enhance triterpenoid content and the biological properties of Vietnamese Ganoderma lucidum extract

“…To determine the factors affecting triterpenoid content from G. lucidum by UAEE, five factors were selected including enzyme concentration, ethanol: material ratio, ultrasonic temperature, ultrasonic time, and ultrasonic power. The selected factors have a survey range based on previous studies (Dat et al, 2021; Huy et al, 2019). Experimental design according to PBD for 5 factors and 12 experiments to determine the influence of each factor on triterpenoid content.…”

Ultrasonic Cell Grinder Extraction of Cyperenoic Acid from Crotonis Crassifolii Radix and the Activity Assay of the Extract

“…For a long time, it has been reported that since multivariable optimisation only focuses on the method of ′one‐factor‐at‐a‐time′, it is impossible to investigate the communication between independent factors to give ambiguous details of the impacts of all determinants. To overcome these shortcomings, response surface methodology (RSM) is introduced as an effective statistical model to estimate the interactions between multiple variables [25–27] . In this study, extraction power, extraction temperature, extraction times and other factors were optimised by RSM.…”