Enzyme-assisted three phase partitioning: A novel approach for extraction of turmeric oleoresin

Global optimization for simultaneous extraction of oil and polysaccharides from Schizochytrium limacinum by enzyme‐assisted three‐phase partitioning

“…Three‐phase Partitioning (TPP) is a relatively new method of biological separation, and the system is divided into three distinct phases by adding t ‐butanol and salt to the crude extract: upper organic phase, in which lipids and hydrophobic substances; proteins in the middle precipitate phase; and bottom phase, in which polar components, such as saccharides (Kurmudle, Bankar, Bajaj, Bule, & Singhal, 2011). TPP technology is currently applied for the extraction of oils, such as from flaxseed (Kulkarni, Kar, & Singhal, 2017); polysaccharides, such as from Corbicula fluminea (Wang, Qiu, Wang, Ma, & Yan, 2017); and protease, such as the milk‐clotting enzyme from zingiber (Gagaoua, Hoggas, & Hafid, 2015) and invertase from potato tubers (Yonca & Erdem, 2014).…”

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“…Preparation of bioactive compounds as a ligand. Bioactive compounds of Zingiberaceae (Lakshmipathy and Kannabiran, 2009;Erukainure et al, 2011;Kiranmayee et al, 2010;Duru and Onyedineke, 2010;Jinsong et al, 2010;Nilesh et al, 2011;Saeed et al, 2010;Cheng et al, 2010;Bhuiyan et al, 2009;Aja et al, 2010) were modeled into three-dimensional structure using ACD Labs software. Three-dimensional shape was obtained by saving it in the 3D viewer in ACDLabs.…”

Enzyme-assisted three-phase partitioning: An efficient alternative for oil extraction from Sesame (<em>Sesamum indicum</em> L.)

“…Following centrifugation, the aqueous phase with carbohydrates, soluble fibers, salts etc. (watersoluble components) is obtained in the lower dense layer, the proteins float in the middle (medium-dense phase) and oil dissolved in t-butanol can be recovered from the upper-most layer also called the oilrich phase (Ruchi et al, 2007;Kurmudle et al, 2011).…”