Enzyme-Assisted Ultrasonic Extraction of Total Flavonoids from Acanthopanax senticosus and Their Enrichment and Antioxidant Properties

Abstract

Flavonoids are one of the important active ingredients from Acanthopanax senticosus, with a variety of biological functions, such as antioxidant and antibacterial properties. The aim of this work was to investigate enzyme-assisted ultrasonic extraction of A. senticosus flavonoids and their enrichment and antioxidant properties. We found that the optimal extraction process after Box–Behnken response surface optimization had the following parameters: a 3:2 ratio of cellulase to pectinase, enzyme mixture amount of 6960 U g−1, enzyme treatment time of 59.80 min; temperature of 53.70 °C, and pH value of 6.05. The yield of total flavonoids reached 36.95 ± 0.05 mg g−1. The results for different polar solvent enrichments showed that the highest flavonoid (61.0 ± 0.344 mg g−1), polyphenol (24.93 ± 0.234 mg g−1), and saponin (17.80 ± 0.586 mg g−1) contents were observed in the 1-butanol fraction, and the highest polysaccharide content (20.04 ± 0.783 mg g−1) was in the water fraction. Pearson correlation analysis revealed that the antioxidant potential of the extract was related to the higher amount of flavonoids and phenolics in the extract. We thus found an effective A. senticosus flavonoid extraction and enrichment procedure, which can serve as a reference method.

Phenolic Profile, Antioxidant, Anti-Enzymatic and Cytotoxic Activity of the Fruits and Roots of Eleutherococcus senticosus (Rupr. et Maxim.) Maxim

“…Examined extracts at a concentration of 0.80 mg/mL, expressed DPPH reduction in the range of 14.7 to 26.2%, with E. gracilistylus as the most active species. In turn, Liu et al [ 32 ] have investigated the potential of chemical compounds possessed by E. senticosus , such as flavonoids, and phenolic acids, and correlated it with its potential to reduce DPPH and ABTS, proving that high concentrations of those components are positively correlated with high antioxidant activity. Those findings stand in line with our current and previous reports [ 19 ].…”

Major Phytochemicals: Recent Advances in Health Benefits and Extraction Method

“…The highest total saponin (11.45%) content was obtained when 5 g of sample was extracted with 75 mL of 73% ethanol at 400 W and 61 °C for 34 min. Liu et al [ 220 ] studied the EAE of saponins from powdered Acanthopanax senticosus . The extraction was carried out at different enzyme concentrations (1000, 5000, and 9000 U/g), time durations (45, 55, and 65 min), temperatures (40, 50, and 60°C), and solvent pH values (5.4, 6, and 6.6).…”

Antioxidant and Antiapoptotic Properties of n‐Butanol Fraction the Acanthopanax senticosus Extracts in H2O2‐RAW264.7 Cells and CCl4‐Induced Liver Injury in Mice

“…Modern pharmacological experiments have demonstrated that A. senticosus possessed antistress, antioxidant, antiapoptotic, and hepatoprotective activities [11]. Previous works showed that the n-butanol fraction of A. senticosus extracts (NASE) has higher antioxidant activity in vitro [12]. As reported in the literature, nbutanol fractions of AS signifcantly prolong the swimming time of mice to exhaustion [13].…”

“…Previous works showed that the n-butanol fraction of A . senticosus extracts (NASE) has higher antioxidant activity in vitro [ 12 ]. As reported in the literature, n-butanol fractions of AS significantly prolong the swimming time of mice to exhaustion [ 13 ].…”