Enzyme‐Based Multi‐Component Optical Nanoprobes for Sequence‐ Specific Detection of DNA Hybridization

Li, Jiang; Song, Shiping; Liu, Xinfeng; Wang, Lihua; Pan, Dun; Huang, Qing; Zhao, Yun; Fan, Chunhai

doi:10.1002/adma.200701918

Cited by 121 publications

(72 citation statements)

References 35 publications

(24 reference statements)

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“…Third, the detection of DNA using this approach is sensitive. We achieve, for example, low picomolar sensitivity for DNA detection, comparing very favorably with previously reported methods (Table 1) (28,35,(50)(51)(52). Fourth, each step in the process (pretreatment with Exonuclease, the binding of the target to the DNA probe, the addition of conjugated polyelectrolytes) is performed separately, allowing each step to be optimized independently.…”

Section: Discussionsupporting

confidence: 62%

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Xia

Zuo

Yang

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

523

348

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We have demonstrated a novel sensing strategy employing single-stranded probe DNA, unmodified gold nanoparticles, and a positively charged, water-soluble conjugated polyelectrolyte to detect a broad range of targets including nucleic acid (DNA) sequences, proteins, small molecules, and inorganic ions. This nearly "universal" biosensor approach is based on the observation that, while the conjugated polyelectrolyte specifically inhibits the ability of single-stranded DNA to prevent the aggregation of gold-nanoparticles, no such inhibition is observed with double-stranded or otherwise "folded" DNA structures. Colorimetric assays employing this mechanism for the detection of hybridization are sensitive and convenient-picomolar concentrations of target DNA are readily detected with the naked eye, and the sensor works even when challenged with complex sample matrices such as blood serum. Likewise, by employing the binding-induced folding or association of aptamers we have generalized the approach to the specific and convenient detection of proteins, small molecules, and inorganic ions. Finally, this new biosensor approach is quite straightforward and can be completed in minutes without significant equipment or training overhead.biosensor | aptamer | visual detection | thrombin detection | cocaine detection G old nanoparticle colorimetric biosensors have seen significant applications in diagnostics, environmental monitoring, and antibioterrorism supporting unaided, visual readout (1-12). Commonly, the relevant nanoparticles are covalently modified with either a probe DNA or an aptamer such that hybridization (13-16) or aptamer-target interactions (17-27), for example the scanometric method developed by Mirkin (25), which is a very sensitive and specific tool, crosslink them, inducing aggregation. The second broad approach utilizes unmodified nanoparticles. (28-30) These two approaches, however, suffer from timeconsuming (20-40 h of assembly) and relatively poor (low nanomolar) detection limits, respectively. Here, a unique, colorimetric sensing strategy employing a simple but selective combination of a single-stranded DNA probe, a positively charged, water-soluble conjugated polyelectrolyte, and unmodified gold nanoparticles is demonstrated. The universality of this method allows detection of a broad range of targets, including nucleic acid (DNA) sequences, proteins, small molecules, and ino rganic ions. Our approach is rapid (turnaround time is 5-10 min) and sensitive (picomolar concentrations of target DNA are readily detected with the naked eye, even in complex sample matrices like blood serum). Hence, an operator with minimum scientific overhead can easily employ this technique.Generally, the gold nanoparticle applications typically rely on a quantitative coupling between target recognition and the aggregation of the nanoparticles, which, in turn, leads to a dramatic change in the photonic properties-and thus the color-of the nanoparticle solution. This colorimetric "readout" avoids the relative complexity inherent...

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Section: Discussionsupporting

confidence: 62%

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Xia

Zuo

Yang

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

523

348

View full text Add to dashboard Cite

show abstract

“…There are many reports on surfactant mixtures regarding their synergism of micellization [25]. In nanoscience, particularly bio-related research, various functional ligands (or surfactant) have been used to impart multi-functionality to the nanoparticle [26,27]. However, the greatly improved dispersion stability by simply mixing two different surfactants with different chain lengths has not been reported yet.…”

Section: Resultsmentioning

confidence: 99%

The simple and facile methods to improve dispersion stability of nanoparticles: Different chain length alkylcarboxylate mixtures

Piao

Lee

Kwon³

et al. 2009

Journal of Colloid and Interface Science

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“…The enhanced activity of an enzyme in presence of a DNA template as well as the prearrangement of reactants on DNA templates could be potentially applied towards increasing the yield of other enzymatic and chemical reactions. DNA-enzyme constructs have been explored previously mainly in the context of sensors [22,23]. The results reported here demonstrate the potential of using DNA templates for accomplishing enzymatic modulation of single step chemical transformations even when the reactants are not covalently attached to the DNA template.…”

Section: Introductionmentioning

confidence: 75%

DNA template-assisted modulation of horseradish peroxidase activity

Datta¹,

Dou²,

Shibley³

et al. 2012

International Journal of Biological Macromolecules

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Enzyme‐Based Multi‐Component Optical Nanoprobes for Sequence‐ Specific Detection of DNA Hybridization

Cited by 121 publications

References 35 publications

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

Colorimetric detection of DNA, small molecules, proteins, and ions using unmodified gold nanoparticles and conjugated polyelectrolytes

The simple and facile methods to improve dispersion stability of nanoparticles: Different chain length alkylcarboxylate mixtures

DNA template-assisted modulation of horseradish peroxidase activity

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