Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Abstract

The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

Oxide nanowire microfluidics addressing previously-unattainable analytical methods for biomolecules towards liquid biopsy

“…6). 69 One major drawback of previous studies was the need for fluorescence labeling, 75 which can affect the biomolecular characteristics. In our recent work, we aimed at label-free monitoring of biomolecular separation using the intensity variation of a laser diffracted using a nanopillar array, 70 and we demonstrated that our proposed nanopillars yielded label-free monitoring and separating of DNA molecules in a nonequilibrium manner.…”