Enzyme-catalyzed assembly of gold nanoparticles for visualized screening of DNA base excision repair

A label-free and sensitive fluorescent method for the detection of uracil-DNA glycosylase activity

“…Nevertheless, these label-free fluorescent approaches for UDG activity detection, 25,26 as well as many labelled probes reported before, [13][14][15][16][17][18] are based on simultaneous removal of multiple uracil bases to induce the conformational changes of DNA for signal transduction. Such DNA substrates containing multiple uracil sites are efficient but may make the estimation of UDG activity relatively complicated, because it can give little signal even if some (not all) of the uracil sites in each DNA substrate are removed but insufficient to induce the DNA conformational changes.…”

“…[13][14][15] Nanomaterials including graphene oxide and gold nanoparticles were also utilized as quenchers and colorimetric reporters, respectively, for optical UDG assays. 16,17 Besides, the activation of uracil-containing DNAzymes by UDG was applied for more sensitive detection of UDG activity through DNAzyme-based signal amplifications. 18,19 Particularly, by incorporating an artificial nucleotide such as 2-aminopurine 20,21 or pyrene 22,23 adjacent to uracil in DNA substrates, fluorescent monitoring of UDG activity was achieved without the use of quenchers, and remarkably the pyrene-based fluorescent probe was successfully applied for the imaging of UDG activity inside living cells.…”

Colorimetric Assay for Uracil DNA Glycosylase Activity Based on Toehold‐Mediated Strand Displacement Circuit

“…Of these, optical methods have garnered great attention due to their intrinsic merits of simplicity, reliability, and sensitivity. The representative examples of optical methods typically rely on the fluorescence resonance energy transfer (FRET), G‐quadruplex DNAzyme, and gold nanoparticles . These methods showed quite good performance offering high sensitivity and selectivity but almost all of them possess several limitations including the requirement for the labeling of signaling molecules such as fluorophore and/or quencher.…”

Tungsten disulfide nanosheet and exonuclease III co-assisted amplification strategy for highly sensitive fluorescence polarization detection of DNA glycosylase activity

“…Gel electrophoresis is the traditional detection methods for DNA glycosylase activity [26,27], which is time-consuming, complicated and of insufficient sensitivity. Consequently, several novel detection methods have been developed to overcome these limits, such as fluorescence resonant energy transfer based detection methods [28e31], G-quadruplex based fluorescent approaches [32] and gold nanoparticle based colorimetric assay [33,34]. Due to the low amounts of DNA glycosylase in real samples, researchers focused on the introduction of signal amplification strategies in the DNA glycosylase activity assays to achieve a high sensitivity, such as DNAzyme-based autocatalytic amplification [35,36], nicking enzyme-assisted signal amplification [37], DNA machine-based amplification [38], and alkaline phosphatase-based enzymatic electrochemical signal amplification [39].…”