Enzyme Composition of Buffalo Seminal Plasma

Chauhan, Rahul; Srivastava, R. K.

doi:10.1530/jrf.0.0340165

Cited by 15 publications

(7 citation statements)

References 5 publications

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“…(1985) estimated the percentage of DS in cockerels in relation to AST in seminal plasma and found that DS were significantly correlated with AST in seminal plasma. Aspartate aminotransferase has been reported to be 166.72, 364.02 and 37.6 U/l in the seminal plasma of buffalo bull, ram and rabbit, respectively (Chauhan and Srivastava 1973; Yousef et al. 2003; Gndoúan 2006).…”

Section: Discussionmentioning

confidence: 99%

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Semen Traits and Seminal Plasma Biochemical Parameters in White Leghorn Layer Breeders

Khan

Laudadio

Tufarelli

2011

Reprod Domestic Animals

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This study was designed to determine the semen quality and seminal plasma biochemical parameters in White Leghorn layer breeders in the early phase of maturity. Individual ejaculates from 25 males were analysed for the determination of volume, sperm concentration, dead sperm percentage (DS) and sperm motility. Seminal plasma was separated and analysed for total oxidant status (TOS), total antioxidant capacity (TAC), paraoxonase (PON1), arylesterase, ceruloplasmin, triiodothyronine, thyroxine, aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Our findings showed a significant (p < 0.05) positive correlation between TOS and DS in layer breeders. The values of TAC were significantly negatively correlated with TOS and DS, while positively correlated with PON1. Conversely, AST showed significant negative correlation with motility and DS. Significantly, negative correlation was also observed between ALT and sperm concentration. In conclusion, these parameters provide some valuable basal data that may help better understanding the semen quality characteristics of White Leghorn layer breeders.

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Section: Discussionmentioning

confidence: 99%

“…Alanine aminotransferase measured in this study was 6.58 U/l. Like AST, ALT has also been reported to be very high in the seminal plasma of buffalo bull, ram and rabbit (Chauhan and Srivastava 1973; Yousef et al. 2003; Gndoúan 2006).…”

Section: Discussionmentioning

confidence: 99%

Semen Traits and Seminal Plasma Biochemical Parameters in White Leghorn Layer Breeders

Khan

Laudadio

Tufarelli

2011

Reprod Domestic Animals

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“…It would be of interest to know the status of these enzymes in buffalo semen when extended in this diluent. Limited reports are available on the level and release of G O T and G P T in buffalo semen (CHAUHAN and SRIVASTAVA, 1973). This paper presents data on the extracellular release of these two enzymes in buffalo semen on freezing in three extenders, namely Citric Acid Whey (GANGULI et al, 1973), Egg-Yolk Citrate (SENGUPTA, 1975) and Tris-Egg Yolk (CHAUBE and SENGUPTA, 1972).…”

Section: Introductionmentioning

confidence: 99%

Extracellular Release of Transaminases from Buffalo Spermatozoa on Freezing of Semen in Extenders

Chinnaiya

Kakar

Ganguli

1979

Zentralblatt für Veterinärmedizin Reihe A

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Summary The levels and release of GOT and GPT in the extracellular fluid of buffalo semen were determined in pre‐freeze, post‐freeze and preserved samples extended with CAW, EYC and TRIS at two dilution rates. Of the three extenders used, the initial value for GOT was higher in semen samples diluted with CAW. The level of GOT was also higher than that of GPT in all samples, irrespective of the extender used. The release of these enzymes was significantly less in CAW‐extended semen in post‐freeze samples, and at the lower dilution rate (1 : 10) GOT release was less. Extended semen on storage in liquid nitrogen also exhibited less release of GOT, unlike GPT release, which was significantly greater in both post‐freeze and stored samples. Zusammenfassung Austritt von Transaminasen aus Büffelspermatozoen in tiefgekühlten und verdünnten Samenproben In Spermaproben von Büffeln wurde der Austritt der GOT und GPT aus den Spermien bzw. die Aktivität dieser Transaminasen in der Extrazellulär‐flüssigkeit bestimmt, und zwar vor und nach der Tiefkühlung sowie nach der Konservierung in CAW (Citric Acid Whey, Citronensäure‐Molken‐Verdünner) —, EYC (Egg‐Yolk‐Citrate, Eigelb‐Citrat‐Verdünner) — und Tris (Tris‐Eigelb‐Verdünner) — Verdünnungsmedien in zwei Verdünnungsgraden. Der Ausgangswert für die GOT war in Spermaproben, welche mit CAW verdünnt wurden, höher als in Proben, welche mit EYC und Tris verdünnt worden waren. In allen Proben war die GOT‐Aktivität höher als diejenige der GPT, wobei die Art des Verdünners keine Rolle spielte. Aufgetaute Proben, die mit CAW verdünnt worden waren, zeigten einen signifikant geringeren Austritt aller drei Enzyme aus den Spermatozoen. Bei weniger stark verdünnten Proben (1 : 10) war der Austritt von GOT geringer. Bei verdünnten Proben, welche in flüssigem Stickstoff aufbewahrt worden waren, war der GPT‐Austritt signifikant größer als derjenige von GOT. Résumé Retrait de transaminases de spermatozoïdes du buffle dans des doses de sperme surgelées et diluées On a déterminé dans des doses de sperme de buffles le retrait de TGO et TGP des spermatozoides et l'activité de ces transaminases dans le liquide extracellulaire. Ces déterminations ont été faites sur deux dilutions avant et après la surgélation ainsi qu'après la conservation dans CAW (Citric Acid Whey), EYC (Egg‐Yolk‐Citrate) et Tris. La valeur pour GTO fut plus élevée dans les doses diluées avec CAW que dans celles avec EYC et Tris. L'activité de TGO fut plus élevée dans toutes les doses que celle de TGP, le diluant n'ayant joué aucun rôle. Les doses dégelées diluées avec CAW ont montré une sortie significativement moins grande des trois enzymes à partir des spermatozoïdes. Le retrait de TGO fut plus faible dans les doses moins fortement diluées (1 : 10). Le retrait de TGP fut significativement plus èlevè que celui de TGO dans les doses diluées conservées dans l'azote liquide. Resumen Salida de transaminasas de los espermatozoides de búfalos en muestras congeladas y diluidas de semen Se valoró en muestras de esperma de búfalos la cesión de...

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“…b Pineda, 2003Gü ndog un, 2006;Andrabi, 2009. c Singh et al, 1969Chauhan and Srivastava, 1973;Javed et al, 2000;Andrabi, 2009. d El-Manna et al, 1986Mosaferi et al, 2005. e Garnica et al, 1993;Juyena, 2011. interactions with proteins present in the epididymal fluid.…”

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confidence: 99%

Seminal Plasma: An Essential Attribute to Spermatozoa

Juyena¹,

Stelletta²

2012

Journal of Andrology

224

169

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The biochemical composition of seminal plasma (SP) is very complex and variable among species. Advances in reproductive technologies reveal SP as a nutritive-protective medium for sperm cells suspended in it, and some SP components are very important for sperm metabolism, as well as sperm function, survival, and transport in the female reproductive tract. Biochemistry of SP is a relatively modern but rapidly expanding field of research, particularly concerning the biological significance of the various biochemical constituents of SP. However, identification of active factors in SP and mechanisms by which they act are not sufficiently understood in different species. Especially in camelids, the origin, composition, and function of the viscous component of SP remain a mystery, and only some biochemical and morphological characteristics of SP have been described. In this review, we focus on the current understanding of physiological and biochemical properties of SP in ruminants and camelids.

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Enzyme Composition of Buffalo Seminal Plasma

Abstract: The preservation quality of buffalo semen is poor. The possibility that the cause is inherent in the enzyme system of the spermatozoa and seminal plasma has not so far been systematically studied. There are a few reports on acid and alkaline phosphatase (Eapen,

Cited by 15 publications

References 5 publications

Semen Traits and Seminal Plasma Biochemical Parameters in White Leghorn Layer Breeders

Semen Traits and Seminal Plasma Biochemical Parameters in White Leghorn Layer Breeders

Extracellular Release of Transaminases from Buffalo Spermatozoa on Freezing of Semen in Extenders

Seminal Plasma: An Essential Attribute to Spermatozoa

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