Enzyme‐coupled fluorescence sensor for sensitive determination of uric acid and uricase inhibitor

Abstract

In this study, an enzyme‐coupled fluorescence sensor was developed successfully for the sensitive detection of uric acid (UA) and uricase inhibitor based on graphene quantum dots (GQDs). UA was first oxidized using uricase and produced hydrogen peroxide (H2O2), which could oxidize phenol to benzoquinone in the presence of horseradish peroxidase (HRP) as the catalysis. Benzoquinone is an efficient quencher and can cause fluorescence quenching of GQDs. The degree of quenching was proportional to UA concentration and was linear for the UA concentration in the ranges 0.2–14 μmol/L. The detection limit was 0.06 μmol/L for UA. In addition, the proposed sensor was further utilized for UA detection in human serum samples with satisfactory reproducibility and accuracy. The proposed strategy provided may be widely utilized to sense H2O2 or H2O2 generating processes in related biological environment.

A novel nanosponge–hydrogel system‐based electrochemiluminescence biosensor for uric acid detection

“…Due to the nanosponge–hydrogel structure, UA molecules were absorbed from serum and decomposed using UAO catalysis to generate hydrogen peroxide. [ 17 ] Consequently, hydrogen peroxide worked as the co‐reactant and reacted with MoS 2 QDs to emit the ECL signal. The detection results demonstrated the advantages of this nanosponge–hydrogel system in the ECL sensor.…”

Tetralactam macrocycle based indicator displacement assay for colorimetric and fluorometric dual-mode detection of urinary uric acid

“…7 c ,8 In the literature, there are reports of optical detection systems which are generally achieved via enzyme-catalysed tandem reactions, which inevitably result in a high price, poor stability and storability, and long reaction time. 9 Therefore, faster and more convenient optical UA detection methods are still needed.…”