Enzyme-functionalized mesoporous silica for bioanalytical applications

Ispas, Cristina; Sokolov, Igor; Andreescu, Silvana

doi:10.1007/s00216-008-2250-2

Cited by 209 publications

(131 citation statements)

References 93 publications

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“…Several examples of enzyme immobilization onto porous silica have been reported [6][7][8][9][10][11][12]. Co-immobilization of enzymes on various platforms is an emerging field, and involves considerations of various factors: spanning from facile access of substrate to the next enzyme in the cascade, optimization of catalytic activity to allow performance of all components, and the stability of enzyme in the solid support [13].…”

Section: Introductionmentioning

confidence: 99%

Stellate MSN-based Dual-enzyme Nano-Biocatalyst for the Cascade Conversion of Non-Food Feedstocks to Food Products

Hsin¹,

Radu²,

Dezayas³

et al. 2017

J Thermodyn Catal

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The successful demonstration of a tandem enzymatic catalyst which utilizes stellate macroporous silica nanospheres (Stellate MSN) platform as dual-enzyme host is reported herein. Upon simultaneous loading of beta-glucosidase and glucose isomerase inside their porous structure, Stellate MSNs-featuring a hierarchical pore arrangement and large surface area, show capability to perform a cascade reaction that converts cellobiose, a cellulosic hydrolysis product, into glucose and further to fructose. The silica platform provides a modality for substrate channelling which involves the transfer of the cascade intermediate, glucose, to the next enzyme without first diffusing to the bulk. A key aspect to this proof-of-concept is the two-enzyme system working in an optimized pH domain to fit the modus operandi for both enzymes. The concept could be extrapolated to other enzyme tandems, with potential to impact dramatically enzymatic processes which require multi-catalyst, one-pot transformations.

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Section: Introductionmentioning

confidence: 99%

Stellate MSN-based Dual-enzyme Nano-Biocatalyst for the Cascade Conversion of Non-Food Feedstocks to Food Products

Hsin¹,

Radu²,

Dezayas³

et al. 2017

J Thermodyn Catal

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“…20)23) Cytochrome c (cyt. c) has catalytic ability in the enantioselective sulfoxidation of aryl alkyl sulfides to optically active sulfoxides, 24) which are of great pharmaceutical interest as chiral intermediates.…”

Section: )12)mentioning

confidence: 99%

“…Mesoporous silica material SBA (SBA 1) and its derivatives (SBA 2 and SBA 3) were prepared using the triblock copolymer EO 20 PO 70 EO 20 (Pluronic P-123; Mn ca. 5800 g/mol, Aldrich), TEOS, and n-decane as a reagent for controlling the morphology (SBA 2) 10) or trimethylbenzene (TMB, Wako) as a swelling reagent for expanding the pore diameter (SBA 3).…”

Section: Mesoporous Silica Materials (Mpss) Synthesismentioning

confidence: 99%

Preparation and catalytic evaluation of cytochrome c immobilized on mesoporous silica materials

Kato

Suzuki

Tanemura

et al. 2010

J. Ceram. Soc. Japan

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Cytochrome c (cyt. c, molecular size of 2.5 nm © 2.5 nm © 3.7 nm) was immobilized on six types of mesoporous silicates (MPS), having pore sizes from 2.8 to 15.0 nm, and their catalytic activities were evaluated by oxidation of thioanisol with H 2 O 2 . Among the silica materials, cyt. c immobilized on MPS pore size of 3.4 nm) showed the best catalytic activity, and its relative activity was improved 1.7 times over that of cyt. c solution. Enhancement of catalytic activity was obtained by immobilization on MPS having a similar size (mesopore) to the molecular size of cyt. c. Activity stability of cyt. c was also evaluated under the various conditions that caused protein inactivation, like HCl, urea, methanol, and guanidine. As results, stability was improved by the immobilization on silica material having a larger pore size (15.0 nm), offered an environment for encapsulating entire cyt. c molecules, inhibiting cyt. c from unfolding and irreversible decomposition.

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“…[14,15] Incorporation of these two components into a suitable carrier allows the formation of a useful hybrid with improved sorption activity and stability [16] and it has been proven that the suitable carriers of hybrid structures are silica materials. [17] High mechanical, chemical and thermal stabilities of silica networks ensure the long-term stability of the final hybrid. [18] There have been a lot of studies focused on environmental remediation using silicaÀchitosan, silicaÀTiO 2 and TiO 2 Àchitosan hybrid materials as adsorbents.…”

Section: Introductionmentioning

confidence: 99%

Structure and properties of innovative silica hybrid materials synthesized for environmental applications

Todorova

Chernev

Okolie

et al. 2015

Biotechnology & Biotechnological Equipment

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Today, environmental protection is one of the main goals in the strive to preserve the human existence. Development in this area requires invention of new materials, which can reduce the levels of pollution. Hybrid materials are suitable for this purpose, because they combine different desirable properties existing in separate sources into one unique and accessible structure. Most of the commonly used materials for the degradation of different kind of pollutants are based on titanium dioxide, because of its photocatalytic activity under UV irradiation. Innovative silica hybrid materials, containing an organic component (chitosan) and titanium nanoparticles, were successfully synthesized via the solÀgel method and tetraethyl orthosilicate was used as a silica source and network former. Interaction between the chitosan and titanium units, and their influence on the structure of final material, were observed and discussed. A homogeneous structure with an even distribution of titanium and chitosan particles was visible from scanning electron microscopy (SEM) micrographs and the particle size varied between 50 and 150 nm. The formed silica network, characteristic peaks of chitosan and titanium groups and possible interactions between them are observed from Fourier transform infrared (FTIR) spectroscopy spectra and nuclear magnetic resonance (NMR) spectroscopy results. The behaviour of the synthesized silica hybrids after thermal treatment was investigated via differential thermal/thermo-gravimetric analysis (DTA/TG) analysis and the sorption and degradation activities of the obtained hybrid materials were investigated using a solution of methyl orange as model pollutant. The structure and properties of the synthesized silica hybrid materials assert their potential application in environmental remediation due to their photocatalytic degradation and sorption activities against pollutants.

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Enzyme-functionalized mesoporous silica for bioanalytical applications

Cited by 209 publications

References 93 publications

Stellate MSN-based Dual-enzyme Nano-Biocatalyst for the Cascade Conversion of Non-Food Feedstocks to Food Products

Stellate MSN-based Dual-enzyme Nano-Biocatalyst for the Cascade Conversion of Non-Food Feedstocks to Food Products

Preparation and catalytic evaluation of cytochrome c immobilized on mesoporous silica materials

Structure and properties of innovative silica hybrid materials synthesized for environmental applications

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