Enzyme histochemistry of the human stomach, with special reference to intestinal metaplasia

Planteydt, H. T.; Willighagen, R. G. J.

doi:10.1002/path.1700800215

Cited by 95 publications

(14 citation statements)

References 20 publications

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“…The enzymatic pattern in our cases of normal stomach agrees with other authors' experiences like P lan teyot and W illig h a g en [15]. C orreia et al [2], R agins et al [16], F loch et al [7].…”

Section: Histochemica! Characteristics O F Atrophic and Hypertrophic supporting

confidence: 82%

“…This is somewhat lower than in other reports where the whole stomach had been thoroughly examined. The enzymatic characteristics of intestinal meta plasia were qualitatively similar to those described previously [13,15]. How ever, marked differences were observed among individual cases in the location and the intensity of the enzyme activity (table III).…”

Section: Histochemica! Characteristics O F Atrophic and Hypertrophic supporting

confidence: 68%

“…C orreia et al [2], R agins et al [16], F loch et al [7]. We observed AcP to be most promi nent in the chief cells like P lan teyot et al [15], C orreia et at. [2], R agins et al [16], and F lo ch et at.…”

Section: Histochemica! Characteristics O F Atrophic and Hypertrophic mentioning

confidence: 54%

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Gastroscopic and Histochemical Study of Normal, Atrophic and Hypertrophic Mucosa

Stoffels¹,

Desneux²,

Gepts³

1972

Digestion

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The correlation between gastroscopic and histological findings was studied in atrophic and hypertrophic gastritis. The histochemical characteristics of these forms of gastritis were determined. 65 stomachs were studied: 15 cases of normal stomach, 40 cases of atrophic gastritis, and 10 cases of hypertrophic gastritis. The biopsy material was obtained in 33 cases by a Crosby capsule, in 5 cases by a Storz biopsy gastroscope, and in 27 cases by laparotomy. The following enzymes were researched: SDH; BDH; LDH; ICDH; G-6-PD; α-GPD; ATPase; Estα; AlkP; AcP. The correlation between gastroscopical observations and histology was excellent, in the cases of atrophic gastritis and of normal stomach, but not in hypertrophic gastritis where in no cases the gastroscopical diagnosis was confirmed by histology. This may be due, as suggested by Schindler, to the type of prelevement (Crosby capsule and biopsy gastroscope). The histoenzymatic study of atrophic gastritis shows: (1) A decrease, in the parietal cells, of the enzymatic activity of several enzymes in 82% of the cases. This decrease was roughly parallel to the intensity of the atrophic changes, and was not equal in the same stomach, and among the different cases. This might be due to the variability of the noxa playing a role in the genesis of gastritis. The reduction, in the parietal cells, of the activity of such enzymes as SDH, ATPase, IDH, and G-6-PD suggests that the lowering of acid output observed in atrophic gastritis may also be due to a lowering of the activity of enzymes playing a part in the energy producing metabolism. (2) The superficial epithelial cells show a decreased enzymatic activity in 65% of the cases. This reduction was also proportional to the importance of the atrophy. The metabolic activity of these cells is probably lessened and, therefore, the mucosa defends itself less firmly. (3) Intestinal metaplasia was found in 50% of the cases. The enzymatic characteristics of intestinal metaplasia were not homogenous, marked differences were observed among individual cases, in the location and the intensity of enzymatic activity. Thus intestinal metaplasia does not appear as an homogenous change. The intestinal metaplasia predisposition to carcinoma is discussed. (4) There was no difference between the enzymatic activity of the chief cells in atrophic gastritis, compared with normal stomach. (5) The enzymatic activity in hypertrophic gastritis was identical to the normal stomach.

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Section: Histochemica! Characteristics O F Atrophic and Hypertrophic supporting

confidence: 82%

Section: Histochemica! Characteristics O F Atrophic and Hypertrophic supporting

confidence: 68%

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Gastroscopic and Histochemical Study of Normal, Atrophic and Hypertrophic Mucosa

Stoffels¹,

Desneux²,

Gepts³

1972

Digestion

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“…In histochemical studies on intestinal marker enzymes in intestinal metaplasia (16,19,24), Abe et al (1) classified intestinal metaplasia into the small intestine type and the colon type and the former was further classified into complete and incomplete types on the basis of the appearance of ALP and LAP activities. By macroscopic demonstration of dissaccharidase activities in the mucosal surface with TES-TAPE, we previously found that there are also two types of intestinal metaplasia, complete and incomplete (7,13,21).…”

mentioning

confidence: 99%

Variety of Phenotypical Expression of Intestinal Marker Enzymes and Mucin in Human Stomach Intestinal Metaplasia

Matsukura

Kawachi

Sügimura

et al. 1980

Acta Histochem. Cytochem

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Intestinal metaplasia in human stomach was classified macroscopically into areas with both sucrase and trehalase activities on the mucosal surface (complete type) and areas with sucrase but no trehalase activities on the mucosal surface (incomplete type) by the TES-TAPE method. Five serial frozen sections were taken from tissues of these two types located with TES-TAPE. The sections were examined histochemically for intestinal marker enzymes and mucin, and the metaplastic tubules were also examined histologically.In mucosa from areas of the complete type, the striated border of almost all the metaplastic tubules gave positive reactions for leucine aminopeptidase (LAP) and alkaline phosphatase (ALP) and contained Paneth's cells. No high-iron diamine (HID) positive mucin was detected by staining with high-iron diamine-Alcian blue (HID-AB). In mucosa from areas of the incomplete type, metaplastic tubules giving a positive reaction for LAP, a HID-positive reaction and a negative reaction for ALP were frequently observed. However, other patterns, such as negative reactions for LAP, ALP and HID, and positive reactions for LAP and ALP, but a HID-negative reaction were also observed.Goblet cells were present but no Paneth's cells were found. Thus a variety of phenotypical expressions of intestinal marker enzymes and mucin were observed in metaplastic tubules, especially in areas of the incomplete type of intestinal metaplasia.Intestinal metaplasia is the replacement of normal stomach epithelium by intestinal epithelium, and it has been studied in relation to gastric cancer (4,14). In humans, well-differentiated adenocarcinomas frequently arise in areas of intestinal metaplasia (15). Moreover, intestinal metalpasia can be induced in rats by chemical carcinogens (10, 11) that induce gastric cancer. Thus, intestinal metaplasia is thought to be the possible beginning of neoplastic change (8).In histochemical studies on intestinal marker enzymes in intestinal metaplasia

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“…On the other hand, it has been reported by many authors (6)(7)(8) that the histoenzymatic patterns in intestinal metaplasia is also identical with that of the small intestine. However, it has been reported by us (l, 2) and Matsumine (5) since a few years ago that the metaplastic epithelium shows more complicated histochemical findings than that pointed out before.…”

mentioning

confidence: 91%

Enzyme Histo- And Biochemistry of Intestinalized Gastric Mucosa

Abe

Ohuchi

Sakano

1974

Acta Histochem. Cytochem

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Seen from the enzyme histochemical patterns, intestinal metaplasia appearing in the gastric mucosa was divided into two types; small intestine type and colon type. Among these types, the incidence of the former was highest.In the typical small intestine type, the patterns of the enzyme reactions in the striated border and cytoplasm were almost the same as in the jejunal villus epithelial cells. In addition, enzymaticall.y atypical cells among the small intestine type cells were frequently seen, too. In the colon type, activities of enzymes in the striated border were very weak or almost negative and cytoplasmic enzymes were moderately reactive resembling the pattern of the colon epithelial cells. In parallel with histochemical study, activities of disaccharidases were biochemically measured on the homogenate of the gastric mucosa. Following the appearance of intestinal metaplasia, activities of maltase and sucrase were significantly elevated showing the pattern of the small intestine, while lactase activity was not increased. In some cases, the ratios of maltase to sucrase activity were distributed in wider range in the intestinalized gastric mucosa than in the small intestine.These enzymochemical findings in intestinal metaplasia were discussed from a viewpoint of cell proliferation and differentiation.

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Enzyme histochemistry of the human stomach, with special reference to intestinal metaplasia

Cited by 95 publications

References 20 publications

Gastroscopic and Histochemical Study of Normal, Atrophic and Hypertrophic Mucosa

Gastroscopic and Histochemical Study of Normal, Atrophic and Hypertrophic Mucosa

Variety of Phenotypical Expression of Intestinal Marker Enzymes and Mucin in Human Stomach Intestinal Metaplasia

Enzyme Histo- And Biochemistry of Intestinalized Gastric Mucosa

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