Enzyme immunoassay techniques an overview

Porstmann, T; Kießig, S.T.

doi:10.1016/0022-1759(92)90061-w

Cited by 400 publications

(238 citation statements)

References 41 publications

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“…The investigator would be well advised to check this point for the particular system under scrutiny. Vast effort has been expended on developing the technique into an immunoassay for the diagnosis of infectious diseases (Wreghitt & MorganCapner, 1990;Porstmann & Kiessig, 1992). At the present level of this application, it is sufficient for the adsorption of the relevant protein (often an antibody) to be qualitatively determined.…”

Section: Enzyme-linked Immunoassay (Elisa)mentioning

confidence: 99%

Experimental methods for investigating protein adsorption kinetics at surfaces

Ramsden¹

1994

Quart. Rev. Biophys.

177

131

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The adsorption of proteins at the solid-liquid interface is a process of fundamental importance in nature. Extensive reviews (MacRitchie, 1978; Andrade & Hlady, 1986; Norde, 1986) testify to the strong interest which has been shown in the problem during the past few decades. Norde & Lyklema (1978) have rightly pointed out that protein adsorption is scientifically intriguing; the phenomenology is complicated and includes many presently apparently irreconcilable observations.

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Section: Enzyme-linked Immunoassay (Elisa)mentioning

confidence: 99%

Experimental methods for investigating protein adsorption kinetics at surfaces

Ramsden¹

1994

Quart. Rev. Biophys.

177

131

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“…Its quality as reagent is evidenced because it kept the biological activity at high dilution (1/12000). The quality parameters analyzed (sensibility, specificity and efficacy) are considered acceptable for immunoenzymatic tests (Porstman & Keessing 1992).…”

Section: Discussionmentioning

confidence: 99%

“…ELISA dependence degree with latex, like reference technique, using Fisher test of the exact probability was estimated. System specificity, efficacy and repeatability were considered as quality indexes (Porstman & Keessing 1992). Absolute sensibility in term of minimum detectable quantity of purified polysaccharide glucuronoxylomannan (GXM) was also calculated using value of blank average plus 2.8 times blank standard deviation (Porstman & Keessing 1992).…”

Section: Methodsmentioning

confidence: 99%

Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

Illnait

Vilaseca

Fernández

et al. 2001

Mem. Inst. Oswaldo Cruz

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“…FACTT was five orders of magnitude more sensitive than ELISA in the detection of recombinant HER2 in the serum. In addition, FACTT detected HER2 in the blood of mice with very early HER2 overexpressing tumor masses, whereas conventional detection methods were unable to detect HER2 in mice bearing tumors as large as 100 mm 3 . Lastly, when human serum samples were subjected to FACTT, individuals with HER2 positive breast cancer (determined by IHC and FISH) were shown to have elevated serum HER2 levels compared to HER2-negative individuals and healthy controls.…”

Section: Her2mentioning

confidence: 96%

“…The enzyme-linked immunosorbent assay (ELISA) is high-throughput and suitable for diagnostic antigen detection; however, it only has a detection limit of 0.01-50 ng/ml [3]. That limit is greatly dependent on antibody affinities, in particular, the capture antibody.…”

Section: Introductionmentioning

confidence: 99%

Non-invasive, ultra-sensitive, high-throughput assays to quantify rare biomarkers in the blood

Freudenberg

Bembas

Greene

et al. 2008

Methods

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Many diseases are easier to treat and control when detected at an early stage of disease progression. Often, disease-related antigens or biomarkers are shed from the primary site and present in the blood. Unfortunately, there are very few tests capable of detecting these rare biomarkers in the blood. A blood test would be very useful to diagnose the disease earlier, monitor effectiveness of treatments, predict recurrence, and monitor recurrence. There is certainly a need to develop assays that are ultrasensitive, non-invasive and high-throughput. Here we describe several highly sensitive immunological assays we have developed to detect rare serum antigens. Initially we created an assay named immunodetection amplified by T7 RNA polymerase (IDAT). To enhance the effectiveness and streamline the procedure, this assay was amended to the facile amplification system termed fluorescent amplification catalyzed by T7 polymerase technique (FACTT). These assays have been used to analyze the tumor antigen HER2 and the prion protein PrP Sc . They can also be applied to other tumor markers or antigens from a variety of diseases such as cardiovascular disease, rheumatoid arthritis, Alzheimer's disease, Parkinson's disease, and hepatitis. These tests are not limited to testing only serum, but may also be applicable to detecting biomarkers in tissue, saliva, urine, cerebrospinal fluid, etc. Clearly, the FACTT-based technology represents an important step in the detection of rare molecules in fluids or tissues for a variety of diseases.

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Enzyme immunoassay techniques an overview

Cited by 400 publications

References 41 publications

Experimental methods for investigating protein adsorption kinetics at surfaces

Experimental methods for investigating protein adsorption kinetics at surfaces

Enzyme-linked immunosorbent assay for detection and quantification of Cryptococcus neoformans antigen

Non-invasive, ultra-sensitive, high-throughput assays to quantify rare biomarkers in the blood

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