Enzyme Immunoassay with Cell Suspensions: Studies on Reactions between Serum Antibodies and Cell-Surface Antigens

Abstract

The following reactions of serum antibodies with cell surface antigens were studied by means of an enzyme immunoassay with cell suspensions: (1) Rh antiserum with human red blood cells; (2) H-2 antisera with murine red blood cells; (3) H-2 antisera with murine thymocytes and hepatocytes; (4) Thy-1 antiserum with murine thymocytes; (5) polyvalent and monovalent HLA alloantisera with human leukocytes and human mononuclear cells, and (6) antisera of murine origin with human and marmoset lymphoid cell lines. In all instances, with the exception of monovalent HLA antisera, the assay proved to be a sensitive and highly specific procedure.

Increased Serum Concentration of the Soluble Interleukin-2 Receptor in Cutaneous T-Cell Lymphoma

“…28 The data are shown as a mean of the duplicate samples (SD, < 10%). The mean nor¬ mal value as determined in the study of 50 healthy subjects (see IL-2R test kit instruction manual) and confirmed in this study (data not shown) was 573 U/mL (upper limit of normal, 919 U/mL; mean+2 SDs).…”