Enzyme immunoassays for the detection of sulfamethazine, sulfadiazine, sulfamethoxypyridazine and trimethoprim in milk

Märtlbauer, Erwin; Meier, Rebecca; Usleber, Ewald; Terplan, G.

doi:10.1080/09540109209354771

Cited by 25 publications

(17 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the European Union and China, the MRL of TMP residues is 50 µg kg −1 in pork, chicken, and fish (Märtlbauer, Meier, Usleber, & Terplan, 1992) (Andrade, de Moraes, Rocha-Filho, Fatibello-Filho, & Cass, 2009). In light of its adverse effects on consumer health, it is important to develop an effective and rapid detection method of TMP residues that can be used to screen a large number of samples.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a monoclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of trimethoprim residues in milk, honey, and fish samples

Liu

Song

Kuang

et al. 2016

Food and Agricultural Immunology

View full text Add to dashboard Cite

A specific monoclonal antibody (mAb) against trimethoprim (TMP) was produced and a mAb-based indirect competitive enzymelinked immunosorbent assay (ic-ELISA) was established to detect TMP residues in milk, honey, and fish samples. The working range of ic-ELISA and IC 50 was 1.83-9.36 and 4.14 µg L −1 . The average recoveries of TMP from TMP-spiked milk, honey, and fish sample were 103.5-118.0%, 100.3-107.1%, and 91.5-108.1%, respectively. The results revealed that our developed mAb-based ic-ELISA can be effectively applied to detect TMP residues in these food products. ARTICLE HISTORY

show abstract

Section: Introductionmentioning

confidence: 99%

Establishment of a monoclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of trimethoprim residues in milk, honey, and fish samples

Liu

Song

Kuang

et al. 2016

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

“…13 However, antibodies used in ELISAs for measuring sulphonamides have been highly speci®c and so cannot be easily used in multiresidue methods. Most published research has focused on sulphamethazine (SMZ) as the target to which antibodies have been raised, and in all cases the most signi®cant crossreactivity of the antibodies raised against SMZ has occurred with sulphamerazine (SMR); Martlbauer et al, 14 Fleeker and Lovett, 15 Haasnoot et al, 16 Singh et al, 17 Hoffmeister et al 18 and McCaughey et al 19 found cross-reactions of 56, 30, 15, 12, 10 and 10% respectively. Endoh et al 20 raised antibodies against sulphamethoxazole (SMX) which were highly speci®c, only sulphathiazole (STZ) giving a signi®cant crossreactivity at 1.5%.…”

Section: Introductionmentioning

confidence: 99%

Atypical antibody specificity: advancing the development of a generic assay for sulphonamides using heterologous ELISA

Spinks¹,

Wyatt²,

Everest³

et al. 2002

J Sci Food Agric

View full text Add to dashboard Cite

Development of immunoassays with broad speci®city recognition of the sulphonamide drugs has proved surprisingly dif®cult in previous work. We have taken an antiserum speci®c for sulphachlorpyridazine in an ELISA system homologous for immunogen and solid phase conjugate and used it in a heterologous ELISA format. The assay proved to have broad speci®city, recognising 15 out of 21 drugs tested. Despite low cross-reactivity (<1%) for seven of the drugs tested, a fully optimised assay could prove useful as a multiresidue method for the group of drugs. The results provide evidence regarding the binding characteristics of the antibody and the immunogenicity of the target.

show abstract

“…Only a very low cross-reactivity was found for sulfamerazine, the sulfonamide which differs only in one methyl function at the pyrimidinyl C-4 position from SMZ. In other experiments, 1,7-9,14 cross-reactivities for SMZ between 10 and 15% were found, except for Märtlbauer et al 12 who reported 56% cross-reactivity.…”

Section: Characterization Of the Polyclonal Antibodiesmentioning

confidence: 81%

“…In this view, immunoassays using antibodies that recognize both the parent drug and its structurally related metabolites are very useful for screening purposes. Application of ELISA for the screening of sulfonamides in feed, 9,10 milk, 11,12 plasma, 7,13 tissue 1,14 or urine 7,14,15 have been described earlier.…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of polyclonal antibodies to sulfamethazine and their potential use in immunoaffinity chromatography for urine sample pre-treatment

Crabbe¹,

Haasnoot²,

Kohen³

et al. 1999

Analyst

View full text Add to dashboard Cite

An immunoaffinity chromatographic (IAC) method for isolating sulfamethazine (SMZ) from incurred urine samples was developed. This was achieved by (i) generating polyclonal antibodies that recognize equally well SMZ and its major urinary metabolites, (ii) evaluating in an ELISA procedure the influence of methanol, salt and pH on the antigen-antibody interaction in order to determine the optimum conditions for IAC and (iii) covalent coupling of the IgG fractions of anti-SMZ to CNBr activated Sepharose for the preparation of re-usable immunoaffinity columns, having a high capacity for SMZ (1900 ng SMZ mL-1 gel). For desorbing SMZ from the immunoaffinity column, different elution modes were evaluated, with 40% MeOH-0.1 mol L-1 HOAc-0.5 mol L-1 NaCl being the most efficient combination. Using the IAC column for processing SMZ spiked urine samples resulted in high recoveries, ranging from 92 to 100%. Because of the high cross-reactivity with the major metabolites of SMZ present in urine of treated animals, the antibodies show excellent properties for use in both IAC and ELISA. For the isolation and concentration of the parent drug and its major metabolites, the urine could be applied directly to the IAC column, without the time-consuming step of deconjugation. Moreover, the use of IAC prior to ELISA for the analysis of incurred urine samples showed good efficiency for the elimination of matrix interferences. Owing to the urine-tissue relationship, the urine concentrations can be used to predict the presence of the parent drug in tissues and so possible violations of the maximum residue limit (MRL) can be controlled.

show abstract

Enzyme immunoassays for the detection of sulfamethazine, sulfadiazine, sulfamethoxypyridazine and trimethoprim in milk

Cited by 25 publications

References 13 publications

Establishment of a monoclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of trimethoprim residues in milk, honey, and fish samples

Establishment of a monoclonal antibody-based indirect enzyme-linked immunosorbent assay for the detection of trimethoprim residues in milk, honey, and fish samples

Atypical antibody specificity: advancing the development of a generic assay for sulphonamides using heterologous ELISA

Production and characterization of polyclonal antibodies to sulfamethazine and their potential use in immunoaffinity chromatography for urine sample pre-treatment

Contact Info

Product

Resources

About