Enzyme-Labeled Pt@BSA Nanocomposite as a Facile Electrochemical Biosensing Interface for Sensitive Glucose Determination

Abstract

Electrocatalytic reactions of glucose oxidation based on enzyme-labeled electrochemical biosensors demand a high enzymatic activity and fast electron transfer property to produce the amplified signal response. Through a "green" synthesis method, Pt@BSA nanocomposite was prepared as a biosensing interface for the first time. Herein we presented a convenient and effective glucose sensing matrix based on Pt@BSA nanocomposite along with the covalent adsorption of glucose oxidase (GOD). The electrocatalytic activity toward oxygen reduction was significantly enhanced due to the excellent bioactivity of anchored GOD and superior catalytic performance of interior platinum nanoparticles, which was gradually restrained with the addition of glucose. A sensitive glucose biosensor was then successfully developed upon the restrained oxygen reduction peak current. Differential pulse voltammetry (DPV) was employed to investigate the determination performance of the enzyme biosensor, resulting in a linear response range from 0.05 to 12.05 mM with an optimal detection limit of 0.015 mM. The as-proposed sensing technique revealed high selectivity against endogenous interfering species, satisfactory storage stability, acceptable durability, and favorable fabrication reproducibility with the RSD of 3.8%. During the practical application in human blood serum samples, this glucose biosensor obtained a good detection accuracy of analytical recoveries within 97.5 to 104.0%, providing an alternative scheme for glucose level assay in clinical application.

MnO2 Submicroparticles from Chinese Brush and Their Application in Treatment of Methylene Blue Contaminated Wastewater

“…Biotemplates like organisms (butterfly wing, 2 hair, 3 wood fiber 4,5 and pollen 6 ), microorganisms (bacteria, 7,8 fungus, 9,10 and viruses 11 ) and biological macromolecules (DNA, [12][13][14] RNA, 15 proteins, [16][17][18][19] and polysaccharides 20 ) were reported to prepare inorganic materials. Among these templates, proteins have gained more popularity by researchers, 21 ranging from ferritin, [22][23][24][25] bovine serum albumin (BSA) [26][27][28][29][30][31] to collagen [32][33][34] . However, proteins from natural extracted or artificial synthetic are difficult to obtain and thus cost a lot.…”

Smart Nanocomposites of Cu-Hemin Metal-Organic Frameworks for Electrochemical Glucose Biosensing

“…Particularly, glucose oxidase (GOD)-based glucose biosensors have been one of the hot spots in analytical chemistry as the introduction of nanomaterials1011.…”

Study of Electrochemical Impedance of a Continuous Glucose Monitoring Sensor and its Correlation With Sensor Performance

“…The electrical impedance of the glucose sensors has been studied in the literature where in particular one impedance parameter (i.e. the charge transfer resistance) is shown to be dependant on the glucose concentration [5], [6] and on the sensor fabrication steps [7].…”