Enzyme-Linked Immunoassay for Detection of Listeria monocytogenes in Dairy Products, Seafoods, and Meats: Collaborative Study

Curiale, Michael S; Lepper, Wendy A.; Robison, Barbara J

doi:10.1093/jaoac/77.6.1472

Cited by 30 publications

(7 citation statements)

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“…Similar detection systems have been reported for Vibrio cholerae (Simonson and Siebeling, 1991), Aeromonas hydrophila (Merino et al, 1993), Staphylococcus aureus (Park et al, 1993), Salmonella species (Patel, 1994), Listeria monocytogenes (Curiale et al, 1996), enteropathogenic Escherichia coli (Chapman et al, 1997) and Bacillus species (Tan et al, 1997) in different kinds of foods.…”

Section: Microorganisms From the Genussupporting

confidence: 70%

Rapid Detection of Pseudomonas in Seafoods Using Protease Indicator

Jabbar

Joishy

1999

Journal of Food Science

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The effects of Pseudomonas and the proteases it synthesizes in seafoods rendering them unfit for consumption are not fully recognized. A sandwich enzymelinked immunosorbent assay (ELISA) was developed wherein protease produced by Pseudomonas isolated from shrimp was used as antigen, and anti-protease IgG conjugated with alkaline phosphatase was the second antibody. Purified protease and seafood samples, naturally contaminated or artificially inoculated with Pseudomonas, were positive by ELISA. The conventional culture method took 3 days to complete, but ELISA detected Pseudomonas within 24h. The rapidity, simplicity and efficacy of this test make it useful for implementation of HACCP systems.

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Section: Microorganisms From the Genussupporting

confidence: 70%

Rapid Detection of Pseudomonas in Seafoods Using Protease Indicator

Jabbar

Joishy

1999

Journal of Food Science

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“…However, results obtained for samples with ‘low’ and ‘high’ contamination levels should be presented for each level separately and not as an overall result. High numbers of target cells in samples, 5–10 colony forming units (cfu) per test portion (usually 25 g), lead to positive test results for rapid methods in most cases [60–62].…”

Section: Detection Of Listeria Monocytogenesmentioning

confidence: 99%

Listeria monocytogenes: diagnostic problems

Beumer

Hazeleger

2003

FEMS Immunology &Amp; Medical Microbiology

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The first isolation methods for the detection of Listeria spp. were generally based on the direct culture of samples on simple agar media, but isolation of the pathogenic Listeria monocytogenes was difficult. In time, new techniques were developed, based on a variety of selective and elective agents in isolation and enrichment media, which gained better and quicker results. Current reference methods allow the recovery of L. monocytogenes from a variety of foods with relative ease. However, more comparative studies are needed to select one horizontal method. It is suggested that the procedure of the International Organization for Standardization is a good base for such comparisons.

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“…Rapid pathogenic bacterial diagnosis has been applied to conduct measurements in biological and food matrix [3]. Up to date, different method has been applied by several research group for enumeration of pathogenic bacteriaespecially L. monocytogenes using polymerase chain reaction immunoassay [4,5], electrochemical sensors [6][7][8], bioluminescence [9,10], DNA-based sensors [11,12], ELISA [13,14], surface plasmon resonance [15,16], fluorescence [17,18], surface-enhanced Raman scattering (SERS) [19][20][21]. It was indicated that the reported methods were optimized to select proper system usage to obtain selectivity and precision, there were some problems such as poor sensitivity and long experimental procedures.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scattering

Akçinar¹,

Aslım²,

Torul³

et al. 2020

Turk J Med Sci

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Background/aim: We aimed to develop a rapid method to enumerate Listeria monocytogenes (L. monocytogenes) utilizing magnetic nanoparticle based preconcentration and surface-enhanced Raman spectroscopy measurements. Materials and methods: Biological activities of magnetic Au-nanoparticles have been observed to have the high biocompatibility, and a sample immunosensor model has been designed to use avidin attached Au-nanoparticles for L. monocytogenes detection. Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. typhimurium) bacteria cultures were chosen for control studies. Antimicrobial activity studies have been done to identify bio-compatibility and bio-characterization of the Au-nanoparticles in our previous study and capturing efficiencies to bacterial surfaces have been also investigated. Results: We constructed the calibration graphs in various population density of L. monocytogenes as 2.2 × 10 1 to 2.2 × 10 6 cfu/mL and the capture efficiency was found to be 75%. After the optimization procedures, population density of L. monocytogenes and Raman signal intensity showed a good linear correlation (R 2 = 0.991) between 10 2 to 10 6 cfu/mL L. monocytogenes. The presented sandwich assay provides low detection limits and limit of quantification as 12 cfu/mL and 37 cfu/mL, respectively. We also compared the experimental results with reference plate-counting methods and the practical utility of the proposed assay is demonstrated using milk samples. Conclusion: It is focused on the enumeration of L. monocytogenes in milk samples and the comparision of results of milk analysis obtained by the proposed SERS method and by plate counting method stay in food agreement. In the present study, all parameters were optimized to select SERS-based immunoassay method for L. monocytogenes bacteria to ensure LOD, selectivity, precision and repeatablity.

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Enzyme-Linked Immunoassay for Detection of Listeria monocytogenes in Dairy Products, Seafoods, and Meats: Collaborative Study

Cited by 30 publications

References 0 publications

Rapid Detection of Pseudomonas in Seafoods Using Protease Indicator

Rapid Detection of Pseudomonas in Seafoods Using Protease Indicator

Listeria monocytogenes: diagnostic problems

Immunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scattering

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